Mda test kit

Manufactured by Solarbio

Sourced in China

The MDA test kit is a laboratory equipment used to detect and quantify the presence of malondialdehyde (MDA), a biomarker commonly associated with oxidative stress. The kit provides a reliable and standardized method for measuring MDA levels in various biological samples.

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Lab products found in correlation

Cinnamaldehyde, by Maclin Biochemical Technology (1 mentions) Reactive oxygen species assay kit, by Solarbio (1 mentions)

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MDA kit (18 citations)

4 protocols using mda test kit

Quantitative MDA Analysis of E. faecalis

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With minor changes, the MDA content was measured using an MDA test kit (Solarbio Life Sciences, Beijing, China) as described by Shao et al. [22 (link)]. The bacterial suspension was prepared according to 2.2 with a final concentration of 2 × 10⁸ CFU/mL. E. faecalis ATCC 29212 cultures were cultivated for 10 min at 37 °C with or without OEO (0 (control), 1/4 MIC, 1/2 MIC, MIC). The bacteria cells were centrifuged (8000× g, 10 min, 4 °C) after incubation and then stored on ice. The extract was added according to the assay kit’s instructions and the cultures were then incubated at 100 °C for 60 min. Finally, the amounts of MDA were measured at 450 nm, 532 nm, and 600 nm using a microplate reader (Infinite™ M200 PRO).
MDA content was calculated according to this equation:

M D A c o n c e n t (n m o l / m L) = 5 \times (12.9 \times (Δ A_{532 nm} - Δ A_{600 nm}) - 2.58 \times Δ A_{450 nm})

Zhan X., Tan Y., Lv Y., Fang J., Zhou Y., Gao X., Zhu H, & Shi C. (2022). The Antimicrobial and Antibiofilm Activity of Oregano Essential Oil against Enterococcus faecalis and Its Application in Chicken Breast. Foods, 11(15), 2296.

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Lipid Peroxidation Quantification Protocol

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Malondialdehyde (MDA), which is the biomarker of lipid peroxidation reaction, was detected according to the protocol of the MDA test kit (Solarbio, Beijing, China) using thiobarbituric acid reactive substances assay (TBARS). Samples were collected at 0.5 h, 2 h, and 4 h, and the absorbance was recorded at 532 nm and 600 nm.

Liu Z., Yu X., Zhou Z., Zhou J., Shuai X., Lin Z, & Chen H. (2023). 3D ZnO/Activated Carbon Alginate Beads for the Removal of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes. Polymers, 15(9), 2215.

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Characterization of Edible Oil Compounds

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Corn oil (>98%) was obtained from Kele Fine Chemical Co., Ltd. (Wuhan, China). Camellia oil (>99%) was obtained from Baicao Pharmaceutical Co., Ltd. (Jian, China). Herring oil (>99%) was obtained from Yongkuo Technology Co., Ltd. (Tianmen, China). Lard was obtained from Angel Yeast Co., Ltd. (Yichang, China). Silicone oil purchased from Boshi Chemical Co., Ltd. (Wuhan, China). Cinnamaldehyde (>97%) and β-CD (>99%) purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). An MDA test kit was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). N-hexane was purchased from Damao Chemical Reagent Co., Ltd. (Tianjin, China). All other reagents were analytical grade.

Liu C., Tian Y., Ma Z, & Zhou L. (2023). Pickering Emulsion Stabilized by β-Cyclodextrin and Cinnamaldehyde/β-Cyclodextrin Composite. Foods, 12(12), 2366.

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ROS and MDA Levels Quantification

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Reactive oxygen species assay kit (CA1410; Solarbio) was used to assess the ROS level in cells. HEK293T cells and HeLa cells as well as stable BRD4 knockdown cells were treated with DMSO or BRD4 inhibitors for 24 h respectively. Rosup, a reagent that can stimulate cells to produce ROS, was diluted to 50 ug/mL by medium, and then added into the cells of Rosup group for 40 min. Then all cells of each group were washed twice with medium and the remaining liquid was sucked away. 100 μL ROS probe DCFH-D with a concentration of 5 μmol/L was added to cells of each group and incubated in a 37 ℃ cell incubator for 20 min. The cells were washed 3 times with serum-free culture solution. Fluorescence intensity was measured at excitation light 488 nm and emission light 525 nm. MDA levels were measured following the instructions of the MDA test kit (BC0025, Solarbio).

Fan C., Guo X., Zhang J., Zheng W., Shi C., Qin Y., Shen H., Lu Y., Fan Y., Li Y., Chen L, & Mao R. (2024). BRD4 inhibitors broadly promote erastin-induced ferroptosis in different cell lines by targeting ROS and FSP1. Discover. Oncology, 15, 98.

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