Hydrogen tetrachloroaurate hydrate

Hydrogen tetrachloroaurate hydrate (HAuCl₄.xH₂O, 99.9%), sodium borohydride (NaBH₄ 99%), silver nitrate (AgNO₃, 99%), hexadecyltrimethylammonium bromide (CTAB, ≥99%), ascorbic acid (99%), sodium oleate (NaOL, ≥99%), Methoxypolyethylene glycol thiol (mPEG-SH) (MW 5000 Da), Dulbecco’s Modified Eagle’s Medium (DMEM), phosphate saline buffer (PBS, Ca²⁺ and Mg²⁺ free, pH = 7.4), and 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich, South Africa and Japan. Hydrochloric acid (HCl, ACS reagent 37 wt.%) was obtained from Wako. Gelatin from porcine skin was obtained from Fluka, Japan. All chemicals were of analytical grade and were used without further purification. All glassware used in the experiment was cleaned and washed thoroughly with MilliQ water (18.2 MΩ cm @ 25 °C) and dried before use.

Hydrogen tetrachloroaurate hydrate; 99.9%, Au 49%- (HAuCl_4∙3H₂ O) was purchased from Sigma-Aldrich. Silver nitrate 99.999% (AgNO3); Glycine, and sodium hydroxide (NaOH) were all purchased from Sigma-Aldrich.

Hydrogen tetrachloroaurate hydrate (99.9%), NaBH₄ (99%), silver nitrate (99%), CTAB (≥99%), ascorbic acid (99%), and NaOL (≥99%) were purchased from Sigma-Aldrich (Kempton Park, South Africa). Hydrochloric acid (ACS reagent, 37 wt%) was obtained from Wako (Osaka, Japan). All chemicals were of analytical grade and were used without further purification. All solutions, other than gold and CTAB, were freshly prepared. The glassware used in the experiments was cleaned and washed thoroughly with MilliQ water and dried before use. A Millipore UV-synergy water purification system of 18.2 MΩ cm @ 25 °C resistivity was used for water production.