Phalloidin tritc conjugate

A mouse monoclonal antibody against M protein and mouse polyclonal antibodies against NP protein and F protein were prepared in our laboratory. Anti-Flag, anti-β-actin, anti-α/β-tubulin, Alexa Fluor 488-conjugated anti-mouse IgG (H+L) and Alexa Fluor 555- conjugated anti-rabbit IgG (H+L) were purchased from Cell Signaling Technology (Beverly, MA, USA). The secondary antibodies against mouse or rabbit used for western blotting were purchased from Bioss Biotechnology (Beijing, China). 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Sigma-Aldrich (St. Louis, MO, USA). CCK-8 cell proliferation and cytotoxicity assay kit were purchased from Solarbio Life Sciences (Beijing, China). CytoD and nocodazole were purchased from Absin Biotechnology (Shanghai, China). Phalloidin-TRITC Conjugate was purchased from AAT Bioquest (Sunnyvale, CA, USA).

The detailed procedures were provided in the supplements. For F‐actin ring staining, osteoclasts on creep plates were incubated with 1 ml PBS containing 1 μl Phalloidin‐TRITC Conjugate (AAT bioquest, AAT‐23102).