Rs 2000 x ray biological irradiator

Manufactured by Rad Source

Sourced in United States

The RS 2000 X-ray Biological Irradiator is a laboratory equipment designed for x-ray irradiation of biological samples. It provides a consistent and controlled x-ray exposure for research and testing purposes.

Automatically generated - may contain errors

Lab products found in correlation

Comet mxr 165 x ray source, by Rad Source (4 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions) Gammacell 220, by Nordion (1 mentions) H1299, by American Type Culture Collection (1 mentions) Cell strainer, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Polymorphpreptm, by Progen Biotechnik (1 mentions) 5 azac, by Merck Group (1 mentions) Be17 516f, by Lonza (1 mentions) Lsrfortessa, by BD (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute 1640 medium, by Thermo Fisher Scientific (1 mentions) Beas 2b, by Thermo Fisher Scientific (1 mentions) Beas 2b, by National Collection of Authenticated Cell Cultures (1 mentions) Hcc827, by National Collection of Authenticated Cell Cultures (1 mentions) H1299, by National Collection of Authenticated Cell Cultures (1 mentions)

26 protocols using rs 2000 x ray biological irradiator

Radiation Exposure in Mice and Xenografts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were exposed to 5.5 Gy total body irradiation using the RadSource Technologies X-ray RS-2000 Biological Irradiator operating at 160 kVp and 25 mA at a dose rate of 2.20 Gy/min. Mice were fed Uniprim for the entire duration of the experiment. Mice were carefully monitored every 2-3 days, and blood was collected from the periorbital sinus of anesthetized mice at the indicated time points. The severity of gastrointestinal syndrome was evaluated by observing body mass loss and performing histological analysis of small intestinal tissue sections. Mice that appeared lethargic and moribund were immediately sacrificed. Apoptotic small intestinal cell death was evaluated by TUNEL staining. Human tumor xenografts were established in the right flank of athymic nude mice. Tumors that reached a volume of ~150mm³ were locally irradiated at fractionated doses of 5 Gy for a total of 6 consecutive days using the RadSource Technologies X-ray RS-2000 Biological Irradiator. Cell lines maintained in tissue culture were irradiated using a Gammacell 220 (MDS Nordion, Ottawa, Canada) ¹³⁷Cs γ-irradiator.

Ranoa D.R., Parekh A.D., Pitroda S.P., Huang X., Darga T., Wong A.C., Huang L., Andrade J., Staley J.P., Satoh T., Akira S., Weichselbaum R.R, & Khodarev N.N. (2016). Cancer therapies activate RIG-I-like receptor pathway through endogenous non-coding RNAs. Oncotarget, 7(18), 26496-26515.

+ Open protocol

+ Expand

Cell Line Cultivation and Irradiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

NSCLC cell lines A549 and H1299 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). HeLa-DR13–9 cells, stably transfected with the pDR-GFP plasmid, were kindly provided by Dr. Jeff Parvin (The Ohio State University). A549 and H1299 cell lines were cultured in RPM I 1640 medium, while HeLa-DR-13–9 cells were cultured in DMEM, supplemented with 10 % of fetal bovine serum (FBS) and 50 U/ml each of penicillin and streptomycin, and maintained in a humidified atmosphere with 5 % CO₂ at 37 °C. For IR treatment, X-ray was delivered to cultured cells in a RS-2000 X-ray Biological Irradiator (Rad Source Technologies, Inc., Suwanee, GA, USA).

Zou N., Xie G., Cui T., Srivastava A.K., Qu M., Yang L., Wei S., Zheng Y, & Wang Q.E. (2016). DDB2 increases radioresistance of NSCLC cells by enhancing DNA damage responses. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37(10), 14183-14191.

+ Open protocol

+ Expand

X-Ray Irradiation of Biological Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were irradiated using an RS2000 X-Ray Biological Irradiator, containing a Comet MXR-165 X-Ray Source (Rad-Source Technologies Inc., Suwanee, GA, USA). A dose of 2.5, 5, 10, or 60 Gy was administered with a dose rate of 0.02 or 0.08 Gy/ s.

Nappo G., Handle F., Santer F.R., McNeill R.V., Seed R.I., Collins A.T., Morrone G., Culig Z., Maitland N.J, & Erb H.H. (2017). The immunosuppressive cytokine interleukin-4 increases the clonogenic potential of prostate stem-like cells by activation of STAT6 signalling. Oncogenesis, 6(5), e342-.

+ Open protocol

+ Expand

Rapid Lyme Disease Chimera Generation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chimeras were generated using a rapid reconstitution protocol that allowed infection within the 8-week window of maximal Lyme arthritis as described previously [25 (link),73 (link)]. C57BL/6 Arf^-/- mice at 5 weeks of age were lethally irradiated with RS-2000 X-ray Biological Irradiator (Rad Source Technologies). Donor splenocytes (2×10⁷) harvested from B6.C3-Bbaa1, B6, and B6 Arf^-/- mice in 200 μl separation buffer (1X PBS pH 7.4, 2% FCS, 2 mM EDTA) were injected intravenously into irradiated B6 Arf^-/- recipient mice 24 h after irradiation. At 3 weeks post-irradiation and transplantation, recipient mice were infected with B. burgdorferi, and Lyme arthritis was assessed at 28 days post-infection. Chimerism was confirmed by PCR analysis of RNA isolated from whole blood at 4 weeks post-infection.

Li J., Ma Y., Paquette J.K., Richards A.C., Mulvey M.A., Zachary J.F., Teuscher C, & Weis J.J. (2022). The Cdkn2a gene product p19 alternative reading frame (p19ARF) is a critical regulator of IFNβ-mediated Lyme arthritis. PLoS Pathogens, 18(3), e1010365.

+ Open protocol

+ Expand

Isolation and Irradiation of Murine and Human Neutrophils

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine neutrophils were isolated from bone marrow, as previously described (10 (link)). Briefly, bone marrow cells were flushed from the femur and tibia with RPMI-1640 (life technologies) +2% Fetal Bovine Serum (FBS, Life technologies). After filtration through a cell strainer (100 µm; BD Falcon, San Jose, CA, USA) and erythrocyte lysis with sterile water, the cells were incubated for 1 h at 37 °C with 5% CO₂ to separate the suspended granulocytes from adherent monocytes.
PolymorphPrep^TM (Progen Biotechnik) was used according to the manufacturer’s instructions, as previously described (9 (link)), to isolate human neutrophils from fresh blood.
For the irradiation assay, neutrophils were irradiated with a dose of 10 Gy using an RS-2000 X-Ray Biological Irradiator (Rad Source Technologies) after being seeded onto 24-well plates.

Lu Y., Jiang H., Li B., Cao L., Shen Q., Yi W., Ju Z., Chen L., Han F., Appelgren D., Segelmark M., de Buhr N., von Köckritz-Blickwede M, & Chen J. (2020). Telomere dysfunction promotes small vessel vasculitis via the LL37-NETs-dependent mechanism. Annals of Translational Medicine, 8(6), 357.

+ Open protocol

+ Expand

Carbon-ion and X-ray Irradiation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbon-ion irradiation was performed at room temperature at the Heavy Ion Accelerator Center (HIRFL) of the Institute of Modern Physics, Lanzhou, Chinese Academy of Sciences (Lanzhou, China), with 300Mev/u monoenergetic carbon ion beams. The penetration depth was adjusted by changing the thickness of the absorber. The cells were irradiated with carbon-ion beams with the LET value 12.6KeV/μm at the entrance plateau. The penetration depths of the ions in water were 15.3 cm (the plateau region of the Bragg curve), and LET value was 31.5 KeV/μm. The cells were irradiated by 0.2 Gy or 2 Gy of carbon-ion beams with the LET value of 12.6 keV/μm or irradiated by 2 Gy of carbon-ion beams with the LET value of 31.5 keV/μm. Three irradiated cells samples were performed for each group.
For X-ray irradiation was performed using the RS2000 X ray biological irradiator (RadSource, Suwanee, GA, USA) at the dose rate of 118 cGy/min. The machine settings: 160 kV 25 mA, 0.3 mm of copper filter, and 45 keV of mean energy.

Wang Y., Guan H., Xie D.F., Xie Y., Liu X.D., Wang Q., Sui L., Song M., Zhang H., Zhou J, & Zhou P.K. (2016). Proteomic Analysis Implicates Dominant Alterations of RNA Metabolism and the Proteasome Pathway in the Cellular Response to Carbon-Ion Irradiation. PLoS ONE, 11(10), e0163896.

+ Open protocol

+ Expand

Ionizing Radiation Exposure Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were irradiated using an RS2000 X-Ray Biological Irradiator, containing a Comet MXR-165x-Ray Source (Rad-Source Technologies Inc., Suwanee, GA, USA). A dose of 60 Gy (STO cells) or 75 Gy (prostate cell lines) was administered with a dose rate of 0.08 Gy/s.

Seed R.I., Taurozzi A.J., Wilcock D.J., Nappo G., Erb H.H., Read M.L., Gurney M., Archer L.K., Ito S., Rumsby M.G., Petrie J.L., Clayton A., Maitland N.J, & Collins A.T. (2019). The putative tumour suppressor protein Latexin is secreted by prostate luminal cells and is downregulated in malignancy. Scientific Reports, 9, 5120.

+ Open protocol

+ Expand

X-ray Irradiation of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

At room temperature, X-ray exposure at a dose rate of 1.18 Gy/min (160 kV, 25 mA, 0.3 mm Cu filter) was applied to cells in the logarithmic growth phase using an RS 2000 X-ray biological irradiator (Rad Source Technologies). Cells were irradiated with doses as indicated (see Results section).

Wang X., Cao Z., Yue X., Liu T., Wen G., Jiang D., Wu W., Le L., Wang Y., Wang C., Wang Z., Jin M., Zhu M., He S., Zhang X., Bu X., Liu R.Y., Peng Z, & Chen Y. (2022). Stabilization of DEPTOR sensitizes hypopharyngeal cancer to radiotherapy via targeting degradation. Molecular Therapy Oncolytics, 26, 330-346.

+ Open protocol

+ Expand

Generation of Bone Marrow Chimeric Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

To generate BM-chimeric mice, 8- to 10-wk-old recipient mice were lethally irradiated (8 Gy) using the RS 2000 X-ray Biological Irradiator (Rad Source Technologies) and reconstituted with three million bone marrow cells from donors by intravenous injection. Prophylactic antibiotics were administered during 1 wk before and the initial 2 wk after transplantation. Chimeras were used for further experiments 6–8 wk after the initial reconstitution.

Chen Q., Yang Y., Hou J., Shu Q., Yin Y., Fu W., Han F., Hou T., Zeng C., Nemeth E., Linzmeier R., Ganz T, & Fang X. (2019). Increased gene copy number of DEFA1/DEFA3 worsens sepsis by inducing endothelial pyroptosis. Proceedings of the National Academy of Sciences of the United States of America, 116(8), 3161-3170.

+ Open protocol

+ Expand

Optimizing Cell Transplantation Outcomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine which preconditioning was optimal for stimulating proliferation of transplanted cells, LEC rat livers underwent ischemia-reperfusion (IRP) and/or radiation (RT) prior to cell transplantation. For the RT procedure, LEC rats were anesthetized with 10% chloral hydrate (3 mL/kg body weight) by intraperitoneal injection. Four days before transplantation, the whole liver received a single dose of 50 Gy at a dose rate of 320 cGy/min using a RS-2000 x-ray Biological Irradiator (Rad Source Technologies, Inc., Suwanee, GA, USA) as described previously [29] (link). For IRP, the left portal vein branch was isolated and occluded with a hemostatic clip for 45 min as described previously [30] (link). Immediately after release of the clip, MSCs^ATP7B were injected into the liver via the portal vein.

Chen S., Shao C., Dong T., Chai H., Xiong X., Sun D., Zhang L., Yu Y., Wang P, & Cheng F. (2014). Transplantation of ATP7B–Transduced Bone Marrow Mesenchymal Stem Cells Decreases Copper Overload in Rats. PLoS ONE, 9(11), e111425.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!