BML-265 was purchased from Enzo Life Sciences. Tyrphostin AG1478 and Erlotinib were ordered from Cayman chemical. DMSO, brefeldin A, Golgicide A and D-biotin were purchased Sigma-Aldrich.
Tyrphostin ag1478
Manufactured by Cayman Chemical
Sourced in United States
Tyrphostin AG1478 is a potent and selective inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. It functions by blocking the ATP-binding site of EGFR, thereby inhibiting its autophosphorylation and downstream signaling.
Automatically generated - may contain errors
Lab products found in correlation
D biotin, by Merck Group (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Golgicide a, by Merck Group (1 mentions)
Erlotinib, by Cayman Chemical (1 mentions)
Mk 2206, by Cayman Chemical (1 mentions)
Glosensor, by Promega (1 mentions)
Ci 1040, by Cayman Chemical (1 mentions)
Gf109203x, by Cayman Chemical (1 mentions)
Hrp conjugated and fluorophore conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Fugene 6 transfection reagent, by Promega (1 mentions)
Protein g sepharose bead, by GE Healthcare (1 mentions)
Nvp aew541, by Cayman Chemical (1 mentions)
Linsitinib, by LC Laboratories (1 mentions)
Erlotinib, by Genentech (1 mentions)
Itraconazole, by Cayman Chemical (1 mentions)
Mycophenolic acid, by Cayman Chemical (1 mentions)
Etravirine, by Cayman Chemical (1 mentions)
Pnu 282987, by Cayman Chemical (1 mentions)
4 protocols using tyrphostin ag1478
1
Molecular Signaling Pathway Inhibitors
2
Ubiquitination Pathway Regulation in Cells
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The primers were synthesized and PAGE purified by IDT DNA. Fugene 6 transfection reagent was purchased from Promega while Lipofectamine RNAiMax was from Life Technologies/Invitrogen. All cell culture reagents including media, antibiotics were from GIBCO/Invitrogen. Antibodies to total Ubiquitin (clone P4D1) was from Santacruz Biotechnology, Luciferase (Millipore), pAKT, AKT (Cell Signaling), Ubiquitin, Lys63 specific (clone Apu3, Millipore), His-tag (clone H3 and C-term, Invitrogen) or Millipore (H8 clone). FLAG tag-HRP antibody (Clone M2) was purchased from Sigma Aldrich. HRP-conjugated and fluorophore-conjugated secondary antibodies were from Jackson ImmunoResearch. ON-TARGET plus siRNA to TRAF6 and scrambled control siRNA were obtained from GE Life Sciences/Dharmacon. D-Luciferin was from Xenogen Corp while GloSensor was from Promega. Erlotinib was a kind gift from Genentech. Tyrphostin AG1478 was obtained from Cayman Chemical. TNF-α antagonist (WP9QY) and IL-1R antagonist were from SantaCruz biotechnology. Recombinant IGF-1, IL-1α were from PepreoTech. CI-1040, GF109203X, NVP-AEW541 and MK2206 (Cayman Chemical), and Linsitinib (LC Laboratories). Protein A and Protein G sepharose beads were from GE HealthCare. Purified NEDD4-1 (Sigma Aldrich), Myc-tagged ubiquitin (Cat. No. U-115) UBE1 (Cat. No. E305), and UBCH5 (Cat. No. E2-616) all were from Boston Biochemicals.
3
Screening of 56 Antischistosomal Compounds
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A total of 56 compounds (Figure S4 , Table S4 ) was selected by both the manual and automated methods for screening
of S. mansoni adults and somules. The following
compounds were purchased from Cayman Chemical: U-73122, BIX01294 hydrochloride
hydrate, tyrphostin AG-1478, sivelestat sodium tetrahydrate, AGK2,
trilostane, itraconazole, dabigatran etexilate, SB202190 hydrochloride,
niflumic acid, amsacrine hydrochloride, R(+)-IAA-94,
piperlongumine, tiamulin fumarate, PNU-282987, tetrabenazine, nemadipine-A,
mycophenolic acid, etravirine, (S)-duloxetine hydrochloride,
moxidectin, and rutecarpine. The following compounds were purchased
from Sigma-Aldrich: clindamycin 2-phosphate, tyrphostin I-OMe-AG-538,
and escabet sodium. The following compounds were purchased from Selleck
Chemicals: revaprazan hydrochloride and Org 27569. The following compounds
were purchased from Enamine: Z385159220, Z18885599, Z276431168, Z2241105867,
Z827016000, Z48867676, Z53005631, Z90192490, Z56174662, Z105384660,
Z44528364, Z827015296, Z304863612, and Z56958732. Finally, the following
compounds were purchased from Toronto Research Chemical: eletriptan
and ondansetron hydrochloride dihydrate. Powders were stored according
to vendor specifications. Compounds were then dissolved at 10 mM in
fresh DMSO and shipped to UCSD on dry ice for storage at −80
°C until use.
of S. mansoni adults and somules. The following
compounds were purchased from Cayman Chemical: U-73122, BIX01294 hydrochloride
hydrate, tyrphostin AG-1478, sivelestat sodium tetrahydrate, AGK2,
trilostane, itraconazole, dabigatran etexilate, SB202190 hydrochloride,
niflumic acid, amsacrine hydrochloride, R(+)-IAA-94,
piperlongumine, tiamulin fumarate, PNU-282987, tetrabenazine, nemadipine-A,
mycophenolic acid, etravirine, (S)-duloxetine hydrochloride,
moxidectin, and rutecarpine. The following compounds were purchased
from Sigma-Aldrich: clindamycin 2-phosphate, tyrphostin I-OMe-AG-538,
and escabet sodium. The following compounds were purchased from Selleck
Chemicals: revaprazan hydrochloride and Org 27569. The following compounds
were purchased from Enamine: Z385159220, Z18885599, Z276431168, Z2241105867,
Z827016000, Z48867676, Z53005631, Z90192490, Z56174662, Z105384660,
Z44528364, Z827015296, Z304863612, and Z56958732. Finally, the following
compounds were purchased from Toronto Research Chemical: eletriptan
and ondansetron hydrochloride dihydrate. Powders were stored according
to vendor specifications. Compounds were then dissolved at 10 mM in
fresh DMSO and shipped to UCSD on dry ice for storage at −80
°C until use.
4
Exploring EGFR and Prostanoid Signaling
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Recombinant human EGF and soluble EGFR were purchased from PeproTech (Rocky Hill, NJ, USA). PGE2, L-798106, PP1 and SU6656 were purchased from Sigma Aldrich. Butaprost (EP2 agonist), Sulprostone (EP3 agonist), L-902,688 (EP4 agonist), Tyrphostin AG-1478 and GM6001 were obtained from Cayman Chemicals (Ann Arbor, MI, USA). H89 and LY294002 were purchased from Calbiochem (Darmstadt, Germany). Go6983 was obtained from Tocris (Bristol, United Kingdom).
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