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Oxo63

Manufactured by GE Healthcare
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Sourced in United States, United Kingdom

The OXO63 is a versatile laboratory equipment designed for various scientific applications. It serves as a centrifuge, providing a means to separate different components of a liquid mixture through the application of centrifugal force.

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Lab products found in correlation

Dotarem, by Guerbet (2 mentions) Matlab software, by MathWorks (1 mentions) Hypersense, by Oxford Instruments (1 mentions) Hypersense hyperpolarizer, by Oxford Instruments (1 mentions)

7 protocols using oxo63

1

EPR Imaging of Tumor Oxygenation

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Tumor pO2 maps were obtained under air and carbogen breathing using an EPR imaging scanner equipped with a volume leg coil resonator tuned to 300 MHz. After the animal was positioned prone with the tumor-bearing leg placed inside the resonator, the EPR oxygen probe (OXO63, GE Healthcare) was injected intravenously as a 1.125-mmol/kg bolus through a cannula placed in the tail vein. EPR signals were obtained following the RF excitation pulses (60 nanoseconds, 80 W, 70° flip angle) using an analog-to-digital converter (200 megasamples/s). The repetition time was 8 microseconds. The free induction decay curves were collected under a nested loop of the x, y, and z gradients, and each time point in the free induction decay curve underwent phase modulation enabling three-dimensional (3D) spatial encoding. Anatomical reference was obtained using a 3-T magnetic resonance imaging (MRI) scanner (MR Solutions, Guildford, UK). The imaging protocol is detailed in Figure 2B. Co-registration of EPR and MRI images and data analysis were accomplished using MATLAB software (Mathworks). From the pO2 images of the tumor, pO2 frequency distributions and median tumor pO2 were calculated.
Neveu M.A., De Preter G., Marchand V., Bol A., Brender J.R., Saito K., Kishimoto S., Porporato P.E., Sonveaux P., Grégoire V., Feron O., Jordan B.F., Krishna M.C, & Gallez B. (2016). Multimodality Imaging Identifies Distinct Metabolic Profiles In Vitro and In Vivo. Neoplasia (New York, N.Y.), 18(12), 742-752.
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2

Synthesis and Characterization of LiNc-BuO

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LiNc-BuO was synthesized according to the literature.(12 (link)) Amifostine was purchased from Yamanouchi Pharmaceutical Co., Ltd. (merged with Fujisawa Pharmaceutical Co., Ltd. in April 2005 as Astellas Pharma Inc., Tokyo, Japan). Carbogen (gas composition of 95% O2 and 5% CO2) was purchased from Takachiho Chemical Industrial Co., Ltd. (Tokyo, Japan). Oxo63 was obtained from GE Health Care (Milwaukee, WI).
Ueno M., Matsumoto S., Matsumoto A., Manda S., Nakanishi I., Matsumoto K.I., Mitchell J.B., Krishna M.C, & Anzai K. (2017). Effect of amifostine, a radiation-protecting drug, on oxygen concentration in tissue measured by EPR oximetry and imaging. Journal of Clinical Biochemistry and Nutrition, 60(3), 151-155.
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3

Hyperpolarized [3-13C]Acetoacetate Protocol

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Approximately 50 mg of lithium [3-13C]acetoacetate, doped with 5.1 mg of trityl-radical (OXO63, GE Healthcare, US), 246 μl of gadoterate meglumine (1.2 mmol/L, Dotarem®, Guerbet, France) and 93 μL of DMSO (25% v/v) was prepared. Total volume is approximately 370 μL, resulting in 1.23 mol/L of [3-13C]acetoacetate, 9.0 mmol/L of OXO63, and 0.80 mmol/L of gadolinium. Then, 200 μL of the sample was hyperpolarized in a polarizer (Hypersense, Oxford Instruments, UK), with 120 min of microwave irradiation at 94.097 GHz. The polarized sample was subsequently dissolved in 3 mL of pressurized and heated Tris-EDTA buffer with pH 7.80, to yield a solution of 80 mmol/L hyperpolarized [3-13C]acetoacetate with a polarization of 10% and physiological temperature and pH.
Abdurrachim D., Woo C.C., Teo X.Q., Chan W.X., Radda G.K, & Lee P.T. (2019). A new hyperpolarized 13C ketone body probe reveals an increase in acetoacetate utilization in the diabetic rat heart. Scientific Reports, 9, 5532.
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4

EPR Imaging of Oxygen Distribution

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Details regarding the specifications of the EPRI spectrometer have been previously published (5 (link),28 (link)). For the tube phantom experiment, 3D EPR data was obtained using three-tube phantom comprised of three tubes containing 2 mM Oxo-63 (GE Healthcare, Waukesha, WI) bubbled with 0%, 2% and 5% oxygen, respectively. Data was encoded using three orthogonal phase-encoding gradients incrementally ramping in 61 equal steps from −40 to 40 mT m-1, resulting in 61×61×61 phase-encoding steps. 581 data points were encoded with a sampling period of 5ns after the minimum RF recovery dead time (530 ns). 8000 averages per phase encoding point and an interpulse delay (TR) of 10 μs was used. A total of 28,373 points (approximately 8-fold undersampling) with a 61×61×61 matrix size were phase-encoded using the hierarchical random sampling strategy.
Jang H., Matsumoto S., Devasahayam N., Subramanian S., Zhuo J., Krishna M.C, & McMillan A.B. (2014). Accelerated 4D Quantitative Single Point EPR Imaging Using Model-based Reconstruction. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 73(4), 1692-1701.
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5

300 MHz Spectrometer for Oxo63 Imaging

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The details of the EPRI system are found in previous publications [15 (link),17 (link),24 (link)]. Briefly, the home built spectrometer operates at 300 MHz. A parallel coil which achieved a Q in the range of 20–25 was used for the imaging experiments. The Trityl-based probe Oxo63 (GE Healthcare, Waukesha, WI) was used as the spin probe. The data were acquired by stepping the spatial encoding gradients from one point to the next in k-space. Each FID was sampled at 581 delay points at a rate of 5 ns after RF recovery dead time of about 350 ns, TR = 6 or 8 μs, RF pulse width = 80 ns.
Chou C.C., Chandramouli G.V., Shin T., Devasahayam N., McMillan A., Babadi B., Gullapalli R., Cherukuri M, & Zhuo J. (2016). Accelerated electron paramagnetic resonance imaging using partial Fourier compressed sensing reconstruction. Magnetic resonance imaging, 37, 90-99.
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6

Isotonic Oxo63 Solution Preparation

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Oxo63 was obtained from GE Health Care (Milwaukee, WI). Figure 1 shows the structure of the Oxo63. Deionized water (deionization by the Milli-Q system) was used for preparing injectable isotonic (75 mM) Oxo63 solution.
Matsumoto K.I., Hyodo F., Mitchell J.B, & Krishna M.C. (2017). Effect of Body Temperature on the Pharmacokinetics of a Triarylmethyl-Type Paramagnetic Contrast Agent Used in EPR Oximetry. Magnetic resonance in medicine, 79(2), 1212-1218.
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7

Hyperpolarized [1-13C]Pyruvate Metabolic Flux

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C-Pyruvate Metabolic Flux Analysis 13 C [1-13C] pyruvic acid (40 mg; Cambridge Isotope Laboratories, MA, USA, #CLM-1082-PK) mixed with 15 mM trityl radical (OXO63, GE Healthcare, Amersham UK) and 1 mM Dotarem (Guerbet, Birmingham UK), was dissolved in saline solution and polarized using a hypersense hyperpolarizer (Oxford Instruments, Oxford UK), as described previously (Lee et al., 2013) . The resulting hyperpolarized [1-13C]pyruvate (0.5 mmol/kg body weight) was injected intravenously within 3 seconds and 60 individual liver spectra were acquired within 1 minute in a 9.4 T preclinical MRI scanner (Lee et al., 2013) .
Caldez M.J., Van Hul N., Koh H.W.L., Teo X.Q., Fan J.J., Tan P.Y., Dewhurst M.R., Too P.G., Talib S.Z.A., Chiang B.E., Stünkel W., Yu H., Lee P., Fuhrer T., Choi H., Björklund M, & Kaldis P. (2018). Metabolic Remodeling during Liver Regeneration. Developmental cell, 47(4).
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