Immunofluorescences on PFA-fixed paraffin embedded tissue slices was performed as in 45 (link). Primary antibodies were anti-cytokeratin (wide spectrum screening, ZO622; Dako), anti-HNF4α (sc-6556; Santa Cruz Biotechnology), anti-SOX9 (AB5535; Millipore) and anti-GFP (ab13970; abcam). Samples were counterstained with ProLong-DAPI (Molecular Probes, Life Technologies) to label cell nuclei. Confocal images were obtained with a Leica TCS SP5 equipped with a CCD camera. Bright field images were obtained with a Nanozoomer Scanner 2.0RS (Hamamatsu).
Zo622
The ZO622 is a compact and versatile laboratory equipment product offered by Agilent Technologies. Its core function is to perform high-precision measurement and analysis tasks within a laboratory environment.
2 protocols using zo622
Immunohistochemistry and Immunofluorescence Analysis of Tissue Samples
Immunofluorescences on PFA-fixed paraffin embedded tissue slices was performed as in 45 (link). Primary antibodies were anti-cytokeratin (wide spectrum screening, ZO622; Dako), anti-HNF4α (sc-6556; Santa Cruz Biotechnology), anti-SOX9 (AB5535; Millipore) and anti-GFP (ab13970; abcam). Samples were counterstained with ProLong-DAPI (Molecular Probes, Life Technologies) to label cell nuclei. Confocal images were obtained with a Leica TCS SP5 equipped with a CCD camera. Bright field images were obtained with a Nanozoomer Scanner 2.0RS (Hamamatsu).
Immunohistochemistry and Immunofluorescence Analysis of Tissue Samples
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