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Cp376395

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The CP376395 is a piece of laboratory equipment manufactured by Bio-Techne. It is designed to perform a specific function in the research and analysis process. The core function of this product is to provide a reliable and accurate tool for researchers and scientists to use in their work. No further details or interpretation of the intended use of this product are provided.

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Lab products found in correlation

Astressin 2b, by Bio-Techne (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Mifepristone, by Merck Group (1 mentions) Metyrapone, by Merck Group (1 mentions) Finasteride, by Merck Group (1 mentions) β cyclodextrin, by Merck Group (1 mentions) 2 hydroxypropyl β cyclodextrin, by Merck Group (1 mentions) Urethane, by Merck Group (1 mentions) Flunixin meglumine, by Merck & Co (1 mentions) Isoflurane, by Merck & Co (1 mentions) Mecamylamine, by Bio-Techne (1 mentions) Stressin 1, by Bio-Techne (1 mentions) Cma 7, by Harvard Apparatus (1 mentions) H 2435, by Bachem (1 mentions)

9 protocols using cp376395

1

Cocaine Addiction CRF Receptor Antagonism

Cited 3 times
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Cocaine HCl was provided by the National Institute on Drug Abuse (Research Triangle Park, NC), and prepared in sterile 0.9% saline solution for intravenous administration. CRF (rat/human; Sigma Aldrich), CP376395 (Tocris Bioscience) and Astressin-2B (Tocris Bioscience) were prepared in aCSF, and stored in 20µL aliquots at −20°C until use. CRF doses were selected based on previous in vivo studies in rats (Kalivas et al. 1987 (link); Blacktop et al., 2011 (link); Wanat et al. 2013 (link)). A lower dose of CRF (50ng) was initially used in order to characterize the dose-response relationship, however, because the 500ng dose of CRF was ineffective in modulating cocaine-taking behavior during the binge-access procedure, only the 50ng dose was further examined (i.e. Experiments 2 and 3). Doses of antagonists were determined based on previous studies conducted in our laboratory (Boyson et al. 2014 (link); Holly et al. 2015 (link); 2016a (link))
Leonard M.Z., DeBold J.F, & Miczek K.A. (2017). Escalated cocaine “binges” in rats: enduring effects of social defeat stress or intra-VTA CRF. Psychopharmacology, 234(18), 2823-2836.
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2

Peptide Reagents for Stress Hormone Studies

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Human CRF (CRF) and human urocortin 1 (UCN1) were purchased from the American Peptide Company or Bachem. Human urocortin 2 (UCN2), human urocortin 3 (UCN3), α‐helical CRF(9‐41), and astressin2B were purchased from the American Peptide Company. CP‐376,395 was purchased from Tocris Bioscience.
Tasma Z., Wills P., Hay D.L, & Walker C.S. (2020). Agonist bias and agonist‐dependent antagonism at corticotrophin releasing factor receptors. Pharmacology Research & Perspectives, 8(3), e00595.
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3

Pharmacological Modulation of Stress Pathways

Cited 3 times
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Ninety-five percent ethyl alcohol (EtOH; Pharmco-AAPER Products) was diluted with tap water to achieve a concentration of 20% EtOH (w/v). EtOH solutions were made weekly and any remaining solution was disposed of at the end of each week.
The CRF-R1 antagonist, CP376395 (Tocris, Minneapolis, MN, USA) was dissolved in 10% dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA) in dH2O, and administered in doses of 10, 17 and 30 mg/kg. Metyrapone (Sigma-Aldrich, St. Louis, MO, USA), an 11β-hydroxylase inhibitor, was dissolved in 0.9% saline and injected in doses of 10, 30 and 50 mg/kg. Twenty percent (2-hydroxypropyl)-β-cyclodextrin (Sigma-Aldrich, St. Louis, MO, USA) in dH2O served as the vehicle for suspensions of the glucocorticoid receptor antagonist, mifepristone (i.e. RU38486; Sigma-Aldrich, St. Louis, MO, USA; 17, 30, 56, and 100 mg/kg). Twenty percent β-cyclodextrin vehicle was also used to prepare suspensions of the 5α-reductase inhibitor, finasteride (Sigma-Aldrich, St. Louis, MO, USA; 10, 30, and 100 mg/kg). Drug doses, selected based on previous research and on pilot studies (Hwa et al. 2016b (link); Lowery et al. 2010 (link); Ramaker et al. 2011 (link)), were prepared immediately prior to their administration and given systemically in an injection volume of 10 mL per kg of body weight.
Newman E.L., Albrechet-Souza L., Andrew P.M., Auld J.G., Burk K.C., Hwa L.S., Zhang E.Y., DeBold J.F, & Miczek K.A. (2018). Persistent escalation of alcohol consumption by mice exposed to brief episodes of social defeat stress: Suppression by CRF-R1 antagonism. Psychopharmacology, 235(6), 1807-1820.
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4

Antagonist Receptor Binding and Anesthesia

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The selective CRF1 receptor antagonist CP376395 (Tocris, Westwoods Business Park, Ellisville, Missouri, USA), corticotropin-releasing factor (CRF) (Sigma-Aldrich, St. Louis, Missouri, USA), and urethane (Sigma-Aldrich) were dissolved in saline (0.9% NaCl). The veterinary pentabiotic (Fort Dodge, Campinas, SP, Brazil), isoflurane (Cristrália, Itapira, São Paulo, Brazil), and the nonsteroidal anti-inflammatory flunixin meglumine (Banamine, Schering-Plough, Cotia, SP, Brazil) were used as supplied by the manufacturers.
Lopes L.M., Reis-Silva L.L., Rodrigues B, & Crestani C.C. (2024). Pharmacological Manipulation of Corticotropin-Releasing Factor Receptors in the Anterior and Posterior Subregions of the Insular Cortex Differently Affects Anxiety-Like Behaviors in the Elevated Plus Maze in Rats. BioMed Research International, 2024, 8322844.
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5

CRFR1 Antagonist Effects on Alcohol Intake

Cited 1 time
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Following testing of baseline alcohol intake, we investigated the effects of a CRFR1 antagonist on alcohol consumption in the HM2 system. One day prior to drug administration, a habituation injection of vehicle (0.9% saline) was administered 30 min prior to the dark cycle. The following day, vehicle or 20 mg/kg CP-376,395 (diluted in 0.9% sterile saline, Batch 1B/226641, Tocris Bioscience, Minneapolis, MN USA) was administered 30 min prior to the dark cycle. All injections were administered at a volume of 10 ml/kg body weight. The dose of 20 mg/kg was selected based on previous work in our lab demonstrating its ability to decrease ethanol intake in singly-housed mice (Giardino and Ryabinin, 2013 (link)). In the socially-housed group, half of the animals (n = 2/cage) received drug while the other half (n = 2/cage) received vehicle, thus allowing administration in a “mixed” setting to more appropriately model treatment-assisted therapy for those undergoing AUD treatment. Intake, drinks, drink size, and channel entries for ethanol and water channels were monitored, where a drink represents an instance in which an animal entered the channel and consumed fluid, drink size represents the average volume (ml) consumed per drink, and a channel entry represents an instance in which an animal entered the channel, but did not consume fluid.
Fulenwider H.D., Robins M.T., Caruso M.A, & Ryabinin A.E. (2021). Social Housing Leads to Increased Ethanol Intake in Male Mice Housed in Environmentally Enriched Cages. Frontiers in Behavioral Neuroscience, 15, 695409.
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6

Intracerebral cocaine administration in rats

Cited 1 time
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CP376395 (Tocris Bioscience) was dissolved in aCSF. The chosen dose had proven to be most effective in similar studies in adult rats (Boyson et al. 2014 (link); Boyson et al. 2011 (link)). Cocaine hydrochloride was obtained from the Research Technology Branch of the National Institute on Drug Abuse (Rockville, MD) and dissolved in sterile 0.9% saline.
Burke A.R., DeBold J.F, & Miczek K.A. (2016). CRF type 1 receptor antagonism in ventral tegmental area of adolescent rats during social defeat: Prevention of escalated cocaine self-administration in adulthood and behavioral adaptations during adolescence. Psychopharmacology, 233(14), 2727-2736.
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7

Reagent Sources for Animal Experiments

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Clozapine N-oxyde was purchased from Enzo Life (France), Stressin I, mecamylamine and CP376395 from Tocris Cookson (UK), Urocortin III from Sigma-Aldrich (France), and Xylazine/Ketamine from Centravet (France). All drugs for in vivo administration were diluted in saline (0.9% NaCl). For bath application of drugs on brain slices, dilution was done in aCSF (artificial cerebrospinal fluid).
Fernandez S.P., Broussot L., Marti F., Contesse T., Mouska X., Soiza-Reilly M., Marie H., Faure P, & Barik J. (2018). Mesopontine cholinergic inputs to midbrain dopamine neurons drive stress-induced depressive-like behaviors. Nature Communications, 9, 4449.
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8

Bilateral VTA CRF Receptor Modulation

Cited 1 time
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Mice were implanted with a dual cannulae system (Plastics One, Roanoke, VA) to bilaterally target the mouse posterior VTA (AP-3.2, ML±0.75, DV-4.5mm from bregma and dura). Dummy cannulae and dust caps fitted the length of the cannula while VTA dual injectors protruded 0.1mm past the cannula. Other groups of mice were implanted with a unilateral VTA cannula and an ipsilateral microdialysis guide cannula (CMA7, Harvard Apparatus, Holliston, MA) targeting the NAc shell (AP+1.7, ML-0.7, DV-4.0mm from bregma and dura). On test days, doses of the CRF-R1 antagonist CP376395 (0.3–0.6µg, Tocris, Ellisville, MO) or CRF-R2 antagonist astressin2B (0.25–0.5µg, Tocris, Ellisville, MO) were freshly dissolved in an artificial cerebral spinal fluid (aCSF) vehicle (0.2µl infused at 0.1µl/min). Doses were chosen based on previous microinjection procedures (Hwa et al. 2013 (link), Boyson et al. 2014 (link)). EtOH and H2O were given to animals 10 min post-infusion. After testing, mice were intracardiacally perfused with 0.9% saline and 4% paraformaldehyde, followed by brain removal. Coronal sections were Nissl stained to check placement of guide cannulae. Animals with incorrect placements into target sites were excluded from analysis.
Hwa L.S., Holly E.N., DeBold J.F, & Miczek K.A. (2015). Social stress-escalated intermittent alcohol drinking: modulation by CRF-R1 in the ventral tegmental area and accumbal dopamine in mice. Psychopharmacology, 233(4), 681-690.
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9

Investigating CRHR1-mediated EPAC activation

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HT22-CRHR1-Epac-S H187 cells were preincubated as described for HT22-CRHR1 cells in ABP-09 uptake experiments. To study the effect of CP-376395 (3212, TOCRIS), preincubation was carried in either 10 or 100 nM concentration of this compound. For control cells DMSO (vehicle) was used in the 20 h preincubation event. FRET experiments were carried out as previously described. 56 The acquisition conditions, image processing protocols and the characteristics of the fluorescence microscope are reported in detail in the SI. Briefly, HT22-CRHR1-EPAC-S H187 cells were seeded in glass-bottom dishes, grown, and incubated with ABP-09, CP-376395 or DMSO, as previously described. After preincubation, cells were stimulated with 10 nM of human/rat CRH (H-2435, Bachem) at time 0. Movies were recorded acquiring images every 15 s, from t = -2 min until t = 15 min, at the spectral channels corresponding to the FRET pair (mTurquoise2 and Venus) and to ABP-09.
Szalai AM ., Armando NG ., Barabas FM ., Stefani FD ., Giordano L ., Bari SE ., Cavasotto CN ., Silberstein S , & Aramendía PF . (2018). A fluorescence nanoscopy marker for corticotropin-releasing hormone type 1 receptor: computer design, synthesis, signaling effects, super-resolved fluorescence imaging, and in situ affinity constant in cells. Physical chemistry chemical physics : PCCP, 20(46).
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