Ten explanted samples of each group were dried at 60 °C, weighed, and hydrolyzed in 14 M HNO3. Calcium quantification was done using a Thermo Solaar M6 atomic absorption spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
Thermo solaar m6
Manufactured by Thermo Fisher Scientific
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The Thermo Solaar M6 is a high-performance atomic absorption spectrometer designed for accurate and reliable elemental analysis. It features a state-of-the-art optical system and advanced signal processing capabilities to provide precise measurements of trace elements in various sample types.
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3 protocols using thermo solaar m6
1
Determination of Calcium Content
2
Calcium Quantification in Explanted Biomaterials
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The explanted 60-day biomaterial samples were dried, weighed, and hydrolyzed in 14 M HNO3. Calcium was quantified using a Thermo Solaar M6 atomic absorption spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
3
Characterization of Heavy Metal Contaminated Soils
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Field-contaminated soils were collected from the surface (0-20 cm) at a lead zinc contaminated mine wasteland (mine soil; 29°24′ N, 102°39′ E) and from farmland near a non-ferrous metal smelter (farmland soil; 30°59′N, 103°57′E) in Sichuan, China. Soil samples were airdried and passed through a 2-mm sieve, and stored in airtight containers prior to analysis. The metal concentrations in the samples were measured using a flame atomic absorption spectrophotometer (FAAS, Thermo Solaar M6, Thermo Fisher Scientific Ltd., USA) after addition of a mixture of concentrated HNO 3 -HCl-HClO 4 at a volume ratio of 1:2:2 in a microwave digestion system (GHZ-16, Beijing Guohuan Institute of High-tech Automation, China). The methods for determining the physicochemical characteristics of the contaminated soils are presented in the Supplementary Information (SI) and the results of the analyses are listed in Table 1. 19.2 ± 1.1 23.9 ± 2.4 Total nitrogen, g kg -1
1.20 ± 0.17 1.51 ± 0.36 Total phosphorus, g kg -1
1.02 ± 0.15 0.790 ± 0.160 Total potassium, g kg -1
13.4 ± 1.1 17.7 ± 0.9 Cation exchange capacity, cmol kg -1 13.0 ± 0. Experimental results are reported as mean ± standard deviation (n = 3).
1.20 ± 0.17 1.51 ± 0.36 Total phosphorus, g kg -1
1.02 ± 0.15 0.790 ± 0.160 Total potassium, g kg -1
13.4 ± 1.1 17.7 ± 0.9 Cation exchange capacity, cmol kg -1 13.0 ± 0. Experimental results are reported as mean ± standard deviation (n = 3).
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