Taqman snp genotyping assay and reverse transcription kit

Anti-GFAP and anti-SOX2 antibodies were obtained from Dako, Agilent (Santa Clara, CA, United States), anti- VDR and anti-aSMase were from Elabscience (Houston, TX, United States) and anti-β tubulin was from Sigma Aldrich (St. Louis, MO, USA). Horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TaqMan SNP Genotyping Assay and Reverse Transcription kit were purchased from Applied Biosystems (Foster City, CA, USA). RNAqueous^®-4PCR kit was from Ambion Inc. (Austin, Texas). SDS-PAGE molecular weight standards were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Chemiluminescence kit was purchased from Amersham (Rainham, Essex, UK).

The rMnSOD radioprotective protein, discovered and obtained in the recombinant form by A.Mancini, was provided by the Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale Tumori Fondazione G. Pascale—IRCCS, Naples, Italy [15 (link)]. Sterile 2 μg aliquots of rMnSOD in 0.5 mL of sterile saline phosphate buffer (PBS) were prepared and stored at −80 °C. Anti-nSMase, and anti-βtubulin were from Abcam (Cambridge, UK). Horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies were from Santa Cruz. Anti-rMnSOD was from InBios International (Washington, WA, USA). TaqMan SNP Genotyping Assay and Reverse Transcription kit were purchased from Applied Biosystems (Foster City, CA, USA). RNAqueous^®-4PCR kit was from Ambion Inc. (Austin, TX, USA). SDS-PAGE molecular weight standards were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Chemiluminescence kits were purchased fromAmersham (Rainham, Essex, UK).

MDA-MCF7 and MDA-MB231 cell lines were from Elabscience Biotechnology (Houston, Texas, USA), and MDA-MD453 was from ATCC (ATCC^® HTB-131, Manassas, VA, USA). Cell culture medium (Dulbecco’s modified minimum essential medium, DMEM), fetal bovine serum (FBS), penicillin G, streptomycin, glutamine, and sodium pyruvate were from GIBCO Invitrogen (Carlsbad, CA, USA). FBS lipid-depleted was from Biowest (Nuaillé, France). Human LDL was from Millipore (Burlington, Massachusetts, USA). Anti-nSMase3, anti-β-tubulin, anti-HMGCR and horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies were from Abcam (Cambridge, UK). ER antibody, clone 6F11, was from Leica Biosystems, Newcastle Upon Tyne, United Kingdom. PgR antibody, clone 16, was from Leica Biosystems, Newcastle Upon Tyne, United Kingdom. Ki-67 antibody clone MIB1 was from Agilent Dako, Glostrup, Denmark. 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-tetrazoliumbromide (MTT), Chol standard, nSMase and were from Sigma-Aldrich (St. Louis, MO, USA). TaqMan SNP Genotyping Assay and Reverse Transcription kit were purchased from Applied Biosystems (Foster City, CA, USA). RNAqueous^®-4PCR kit was from Ambion Inc. (Austin, TX, USA). SDS-PAGE molecular weight standards were purchased from Nzythech (Lisboa, Portugal). Chemiluminescence kits were purchased from Amersham (Rainham, Essex, UK).