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Chemiluminescent immunoassay

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Chemiluminescent immunoassay is a type of laboratory equipment used for the detection and quantification of specific analytes, such as proteins, hormones, or other biological molecules, in a sample. The core function of this technology is to use a chemiluminescent reaction to generate a light signal that is proportional to the concentration of the target analyte, allowing for accurate and sensitive measurement.

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Lab products found in correlation

Chemiluminescent microparticle immunoassay, by Abbott (2 mentions) Glucose hexokinase method, by Bayer (2 mentions)

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Labelled chemiluminescent immunoassay (2 citations) Chemiluminescent immunoassay kit (2 citations) Two-site chemiluminescent immunoassay (2 citations) Solid-phase competitive chemiluminescent enzyme immunoassay (2 citations)

8 protocols using chemiluminescent immunoassay

1

Serum Vitamin D and PTH Measurement

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Serum 25OHD concentrations were measured by chemiluminescent microparticle immunoassay (Abbott, Architect, India; Coefficient of variance 3.7% ±0.7%) and serum parathyroid hormone (PTH) concentrations were measured by chemiluminescent immunoassay (Siemens, India; coefficient of variance 3%).
Patel P.P., Patel P.A., Zulf M.M., Yagnik B., Kajale N., Mandlik R., Khadilkar V., Chiplonkar S.A., Phanse S., Patwardhan V., Joshi P., Patel A, & Khadilkar A.V. (2017). Association of dental and skeletal fluorosis with calcium intake and serum vitamin D concentration in adolescents from a region endemic for fluorosis. Indian Journal of Endocrinology and Metabolism, 21(1), 190-195.
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2

Oral Glucose Tolerance Test Protocol

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After an overnight fast, a 20-gauge polyethylene catheter was placed in an antecubital vein for blood sampling. Blood samples were drawn at different time points before (10 and 0 min) and after (30, 60, 90, and 120 min) a 75-g glucose ingestion for the determination of plasma glucose and insulin. Plasma glucose was measured with the glucose hexokinase method (Bayer Diagnostics, Tarrytown, NY). Plasma insulin was determined by a chemiluminescent immunoassay (Siemens, Malvern, PA).). The estimate of insulin sensitivity by homeostasis model assessment (HOMA) score was calculated with the following formula: fasting plasma insulin (U/ml) × glucose (mmol/l) / 22.5, which is equal to fasting insulin (pmol/l) × glucose (mg/dl) 2,815 after unit conversions (15 (link)). Glucose and insulin areas during OGTT were determined with Tai’s model: 1/2 × 30 × (y0min + 2y30min + 2y60min + 2y90min + y120min), where y represents insulin or glucose concentrations at the different time points (16 (link)).
You T., Wang X., Murphy K.M., Lyles M.F., Demons J.L., Yang R., Gong D.W, & Nicklas B.J. (2014). Regional Differences in Adipose Tissue Hormone/Cytokine Production Before and After Weight Loss in Abdominally Obese Women. Obesity (Silver Spring, Md.), 22(7), 1679-1684.
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3

Oral Glucose Tolerance Test Protocol

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After an overnight fast, a 20-gauge polyethylene catheter was placed in an antecubital vein for blood sampling. Blood samples were drawn at different time points before (10 and 0 min) and after (30, 60, 90, and 120 min) a 75-g glucose ingestion for the determination of plasma glucose and insulin. Plasma glucose was measured with the glucose hexokinase method (Bayer Diagnostics, Tarrytown, NY). Plasma insulin was determined by a chemiluminescent immunoassay (Siemens, Malvern, PA).). The estimate of insulin sensitivity by homeostasis model assessment (HOMA) score was calculated with the following formula: fasting plasma insulin (U/ml) × glucose (mmol/l) / 22.5, which is equal to fasting insulin (pmol/l) × glucose (mg/dl) 2,815 after unit conversions (15 (link)). Glucose and insulin areas during OGTT were determined with Tai’s model: 1/2 × 30 × (y0min + 2y30min + 2y60min + 2y90min + y120min), where y represents insulin or glucose concentrations at the different time points (16 (link)).
You T., Wang X., Murphy K.M., Lyles M.F., Demons J.L., Yang R., Gong D.W, & Nicklas B.J. (2014). Regional Differences in Adipose Tissue Hormone/Cytokine Production Before and After Weight Loss in Abdominally Obese Women. Obesity (Silver Spring, Md.), 22(7), 1679-1684.
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4

Seasonal Variation in Vitamin D and PTH

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To minimize the seasonal variation and change in sunlight exposure, all samples were obtained during the same season, i.e., during spring (March–April 2014). After an overnight fast, blood samples were collected between 7:30 and 8:00 am in the morning. Serum 25(OH)D concentrations were measured by chemiluminescent microparticle immunoassay (Abbott, Architect, India; Coefficient of variance 3.7% ± 0.7%). Intact serum parathyroid hormone (PTH) concentrations were measured by chemiluminescent immunoassay (Siemens, India; coefficient of variance 3%). Serum lipid concentrations were measured using Siemens auto analyzer (Date dimension RXL Max) with enzymatic procedures for the measurement of TC (<220 mg/dl), triglycerides (TG) (<150 mg/dl).
Patel P.A., Patel P.P., Mughal Z., Padidela R., Patel A.D., Patwardhan V., Chiplonkar S.A., Khadilkar V, & Khadilkar A. (2017). Interrelationship between serum 25-hydroxyvitamin D3 concentration and lipid profiles in premenopausal Indian women. Indian Journal of Endocrinology and Metabolism, 21(1), 96-101.
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5

Comprehensive Hormonal Profiling Protocol

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Sera were assayed at the Human Nutritional Chemistry Service Laboratory at Cornell University for luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, insulin, and sex hormone binding globulin (SHBG) using a chemiluminescent immunoassay (Siemens Medical Solutions Diagnostics, Deerfield, IL). The intra-assay coefficients of variation (CVs) ranged from 3 to 6% and the inter-assay CVs ranged from 5 to 10%. Serum total testosterone was measured using an LC-MS/MS assay (Brigham and Women’s Hospital Research Assay Core Boston, MA), which has been certified by the Centers for Disease Control and Prevention HoST Program. The inter-assay CV was <8% and intra-assay CV was <5%. Free and bioavailable testosterone were calculated with methods described previously [30 (link)] and can also be accessed via the internet at: http://www.issam.ch/freetesto.htm (last accessed on 17 January 2023).
Vanden Brink H., Jarrett B.Y., Pereira N., Spandorfer S.D., Hoeger K.M, & Lujan M.E. (2023). Diagnostic Performance of Ovarian Morphology on Ultrasonography across Anovulatory Conditions—Impact of Body Mass Index. Diagnostics, 13(3), 374.
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6

Measurement of Serum IGF-1, 25(OH)D, and PTH

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Serum total IGF-1 was measured by a validated [21 (link)] chemiluminescent immunoassay (Siemens Medical Solutions Diagnostics, New York, NY, USA) in both the BLSA and the MAGIC samples. The inter-assay coefficient of variation (CV) was 8.4% and the lower detection limit was 20 ng/mL Concentrations of 25(OH)D, the marker of vitamin D status, were assessed by liquid chromatography-mass spectrometry at Mayo Clinic laboratories (Rochester, MN, USA) in the BLSA and by chemiluminescent immunoassay on the DiaSorin Liaison® System (DiaSorin, Saluggia, Italy) in the MAGIC population. The inter-assay CV was 10% and 12.6%, respectively, while the lower detection limit was 4 ng/mL for both assays. Previous head-to-head comparisons demonstrated a good overall agreement between 25(OH)D immunoassays and the liquid chromatography-mass spectrometry method [22 (link)].
Serum levels of intact PTH were measured by chemiluminescent immunoassay in the BLSA (Siemens Medical Solutions Diagnostics, New York, NY, USA) and by a third generation sandwich-type immunoassay specific for human 1–84 PTH in the MAGIC cohort (DiaSorin, Saluggia, Italy). The inter-assay CV was 8% for both assays.
Ameri P., Canepa M., Fabbi P., Leoncini G., Milaneschi Y., Mussap M., AlGhatrif M., Balbi M., Viazzi F., Murialdo G., Pontremoli R., Brunelli C, & Ferrucci L. (2014). Vitamin D modulates the association of circulating insulin-like growth factor-1 with carotid artery intima-media thickness. Atherosclerosis, 236(2), 418-425.
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7

Serum hs-CRP Determination Protocol

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Blood serum samples were obtained within seven days after the index event. Chemiluminescent immunoassay (Siemens) was used to determine the concentration of hs-CRP. Detection limits ranged from 0.3–101 mg/L with an analytical sensitivity of 0.1 mg/L and a functional sensitivity of 0.3 mg/L.
Schaeff V.L., Sperber P.S., Piper S.K., Giesers N.K., Gertz K., Heuschmann P.U., Endres M, & Liman T.G. (2023). Associations of C-reactive protein with depressive symptoms over time after mild to moderate ischemic stroke in the PROSCIS-B cohort. Journal of Neurology, 271(2), 909-917.
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8

Quantification of Serum IGF-1 Levels

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IGF-1 levels were measured using a chemiluminescent immunoassay (Siemens, Surrey, UK), reporting IGF-1 levels between 25–1600 ng/ml. Age-adjusted reference and sex-adjusted reference ranges were used. IGF-1 standard deviation scores (SDS) were calculated using a standardised reference table from the assay manufacturer.
Thomas J.D., Dattani A., Zemrak F., Burchell T., Akker S.A., Gurnell M., Grossman A.B., Davies L.C, & Korbonits M. (2016). Characterisation of myocardial structure and function in adult-onset growth hormone deficiency using cardiac magnetic resonance. Endocrine, 54(3), 778-787.
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