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Rna seq reads

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RNA-seq reads are raw sequencing data obtained from an RNA sequencing experiment. They represent the genetic material present in a biological sample, captured as short nucleotide sequences. These reads can be used for various analyses, such as gene expression profiling, transcript identification, and differential expression studies.

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Lab products found in correlation

Iso seq, by Pacific Biosciences (1 mentions)

2 protocols using rna seq reads

1

Aedes aegypti Genome Annotation

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The AaegL5 assembly was deposited at NCBI in June 2017 and annotated using the NCBI RefSeq Eukaryotic gene annotation pipeline56 . Evidence to support the gene predictions came from over 9 billion Illumina RNA-seq reads, 67k Pacific Biosciences IsoSeq reads, 300k ESTs, and well-supported proteins from D. melanogaster and other insects. Annotation Release 101 was made public in July 2017, and specific gene families were subjected to manual annotation and curation. Detailed descriptions of the manual annotation and curation of multigene families (hox genes, proteases, opsins and biogenic amine receptors, chemosensory receptors, and ligand-gated ion channels) are in Supplementary Methods and Discussion. See also: https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Aedes_aegypti/101/
Matthews B.J., Dudchenko O., Kingan S.B., Koren S., Antoshechkin I., Crawford J.E., Glassford W.J., Herre M., Redmond S.N., Rose N.H., Weedall G.D., Wu Y., Batra S.S., Brito-Sierra C.A., Buckingham S.D., Campbell C.L., Chan S., Cox E., Evans B.R., Fansiri T., Filipović I., Fontaine A., Gloria-Soria A., Hall R., Joardar V.S., Jones A.K., Kay R.G., Kodali V.K., Lee J., Lycett G.J., Mitchell S.N., Muehling J., Murphy M.R., Omer A.D., Partridge F.A., Peluso P., Aiden A.P., Ramasamy V., Rašić G., Roy S., Saavedra-Rodriguez K., Sharan S., Sharma A., Smith M.L., Turner J., Weakley A.M., Zhao Z., Akbari O.S., Black WC I.V., Cao H., Darby A.C., Hill C.A., Johnston J.S., Murphy T.D., Raikhel A.S., Sattelle D.B., Sharakhov I.V., White B.J., Zhao L., Aiden E.L., Mann R.S., Lambrechts L., Powell J.R., Sharakhova M.V., Tu Z., Robertson H.M., McBride C.S., Hastie A.R., Korlach J., Neafsey D.E., Phillippy A.M, & Vosshall L.B. (2018). Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature, 563(7732), 501-507.
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2

Aedes aegypti Genome Annotation Release 101

Check if the same lab product or an alternative is used in the 5 most similar protocols
The AaegL5 assembly was deposited at NCBI in June 2017 and annotated using the NCBI RefSeq Eukaryotic gene annotation pipeline56 . Evidence to support the gene predictions came from over 9 billion Illumina RNA-seq reads, 67,000 Pacific Biosciences IsoSeq transcripts, 300,000 expressed sequence tags and well-supported proteins from D. melanogaster and other insects. Annotation Release 101 was made public in July 2017, and specific gene families were subjected to manual annotation and curation. Detailed descriptions of the manual annotation and curation of multigene families (Hox genes, proteases, opsins and biogenic amine receptors, chemosensory receptors and LGICs) can be found in the Supplementary Methods and Supplementary Discussion. See also https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Aedes_aegypti/101/.
Matthews B.J., Dudchenko O., Kingan S.B., Koren S., Antoshechkin I., Crawford J.E., Glassford W.J., Herre M., Redmond S.N., Rose N.H., Weedall G.D., Wu Y., Batra S.S., Brito-Sierra C.A., Buckingham S.D., Campbell C.L., Chan S., Cox E., Evans B.R., Fansiri T., Filipović I., Fontaine A., Gloria-Soria A., Hall R., Joardar V.S., Jones A.K., Kay R.G., Kodali V.K., Lee J., Lycett G.J., Mitchell S.N., Muehling J., Murphy M.R., Omer A.D., Partridge F.A., Peluso P., Aiden A.P., Ramasamy V., Rašić G., Roy S., Saavedra-Rodriguez K., Sharan S., Sharma A., Smith M.L., Turner J., Weakley A.M., Zhao Z., Akbari O.S., Black WC I.V., Cao H., Darby A.C., Hill C.A., Johnston J.S., Murphy T.D., Raikhel A.S., Sattelle D.B., Sharakhov I.V., White B.J., Zhao L., Aiden E.L., Mann R.S., Lambrechts L., Powell J.R., Sharakhova M.V., Tu Z., Robertson H.M., McBride C.S., Hastie A.R., Korlach J., Neafsey D.E., Phillippy A.M, & Vosshall L.B. (2018). Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature, 563(7732), 501-507.
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