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Polyclonal raco 1 hpa030098 and trim7 hpa039213 antibodies

Manufactured by Merck Group
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Polyclonal RACO-1 (HPA030098) and Trim7 (HPA039213) antibodies are laboratory reagents produced by Merck Group. The RACO-1 antibody is a polyclonal antibody, while the Trim7 antibody is also a polyclonal antibody. These antibodies are intended for use in research applications.

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Lab products found in correlation

Jun 610326, by BD (2 mentions) Flag a8592 and ha h 6908, by Merck Group (2 mentions) Gst sc 459, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Polyclonal antibodies (4 citations) HPA030098 (2 citations)

2 protocols using polyclonal raco 1 hpa030098 and trim7 hpa039213 antibodies

1

Characterization of RACO-1 and TRIM7 in Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human embryonic kidney (HEK) 293T cells, human lung adenocarcinoma H727 cells and primary murine lung adenocarcinoma cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS). HEK293T and H727 cells were obtained from Cancer Research UK Cell Services. Polyclonal RACO-1 (HPA030098) and Trim7 (HPA039213) antibodies were from Sigma. Antibodies against p-ERK1/2 (9106), total ERK1/2 (9102), pCREB (9191), total CREB (9197), MSK1 (3489) and MSK2 (3679) were from Cell Signaling Technology; Jun (610326) from BD transduction laboratories; Flag (A8592) and HA (H 6908) from Sigma; β-actin (ab49900), GFP (ab290) from Abcam; and GST (sc-459) from Santa Cruz. Mouse monoclonal antibody against Myc tag was generated in-house. Anti-FLAG HRP antibody was used at 1:5000 dilution and anti- β-actin HRP antibody was used at 1:30,000 dilution. All other antibodies were used at 1:1000 dilution for western blots. Uncropped scans of western blots are shown in Supplementary Fig. 6.
Chakraborty A., Diefenbacher M.E., Mylona A., Kassel O, & Behrens A. (2015). The E3 ubiquitin ligase Trim7 mediates c-Jun/AP-1 activation by Ras signalling. Nature communications, 6, 6782.
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2

Characterization of RACO-1 and TRIM7 in Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human embryonic kidney (HEK) 293T cells, human lung adenocarcinoma H727 cells and primary murine lung adenocarcinoma cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS). HEK293T and H727 cells were obtained from Cancer Research UK Cell Services. Polyclonal RACO-1 (HPA030098) and Trim7 (HPA039213) antibodies were from Sigma. Antibodies against p-ERK1/2 (9106), total ERK1/2 (9102), pCREB (9191), total CREB (9197), MSK1 (3489) and MSK2 (3679) were from Cell Signaling Technology; Jun (610326) from BD transduction laboratories; Flag (A8592) and HA (H 6908) from Sigma; β-actin (ab49900), GFP (ab290) from Abcam; and GST (sc-459) from Santa Cruz. Mouse monoclonal antibody against Myc tag was generated in-house. Anti-FLAG HRP antibody was used at 1:5000 dilution and anti- β-actin HRP antibody was used at 1:30,000 dilution. All other antibodies were used at 1:1000 dilution for western blots. Uncropped scans of western blots are shown in Supplementary Fig. 6.
Chakraborty A., Diefenbacher M.E., Mylona A., Kassel O, & Behrens A. (2015). The E3 ubiquitin ligase Trim7 mediates c-Jun/AP-1 activation by Ras signalling. Nature communications, 6, 6782.
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