Gentamycin sulfate
Gentamycin sulfate is a laboratory reagent used in cell culture and microbiology applications. It is an antibiotic that inhibits bacterial growth and is commonly used to prevent microbial contamination in cell culture systems.
Lab products found in correlation
11 protocols using gentamycin sulfate
Culturing Murine Cancer Cell Lines
Spleen Tissue Lysate Preparation
The tissue slice was placed on a sterile sieve positioned on top of a sterile dish prior to being crushed using a pestle allowing the debris to remain on the sieve and the supernatant to pass onto the dish. The sieve was rinsed using 500 µl sterile PBS (pH7.0) containing 5 µg/ml gentamycin sulfate (Bio Whittaker) allowing the lysate to drain onto the dish. The tissue lysate containing PBS was then pipetted out of the dish and transferred into a sterile 1.5 ml Eppendorf tube. The lysate was centrifuged at 14,000 rpm for 10 min to remove tissue debris and the resultant supernatant was pipetted out and transferred into a sterile 1.5 ml Eppendorf tube and stored at − 20 °C. The lysate was used for both extraction of genomic DNA for diagnostic confirmation, for genotyping and as an inoculum for virus isolation.
Propagation of PEDV strain 13-019349 in Vero-81 cells
Naïve CD8+ T Cell Expansion by IL-15
Bronchoalveolar Lavage Cell Isolation
COS-7 Cell Culture Protocol
Isolation and Expansion of Treg Subsets from ANCA-GPA Patients
Cell Culture Protocol for Various Cell Lines
HEK 293-T, B16F10, B16.SIY, and MC-38 cells were cultured in Dulbecco’s Modified Eagle’s Medium (ATCC) supplemented with 10% fetal bovine serum (FBS, R&D Systems), 100 U/mL penicillin (Thermo), and 100 μg/mL streptomycin (Thermo). TC-1, DC2.4, and 58-/- lines were cultured in RPMI-1640 media (ATCC) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. All mammalian cell lines and assay cultures were maintained at 37°C and 5% CO2. All cells were frequently tested and confirmed negative for mycoplasma contamination. B16F10 cells used for tumor challenge studies also tested negative for rodent pathogens.
Sf9 cells were cultured in Sf-900 III SFM (Gibco) supplemented with L-glutamine, 10% FBS (R&D Systems), and 20 μg/mL gentamicin sulfate (Lonza). Hi5 cells were cultured in Insect-XPRESS (Lonza) supplemented with 10 μg/mL gentamycin sulfate (Lonza). Insect cell lines were maintained at 27°C with shaking at 120 rpm.
Differentiation of Human Subcutaneous Adipocytes
Whey Hydrolysate Modulates NF-κB Cytokine Production
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