5 mcg of carrier-free human VEGF165 (R&D Systems, Inc.) was iodinated with 1mCi of Na125I as described [21 (link)]. Labeled VEGF was then separated from free 125I by passage over a Sephadex G25M PD-10 and Hi-Trap Heparin columns (Pharmacia, Alameda, CA). This yielded a solution that contained 99% of counts in the bound (125I-VEGF) form based on precipitation with cold 10% TCA. Specific activity was 1909 Ci/mMole. Purity was confirmed by migration of a single 22 kDa band (monomer) on reducing SDS-PAGE autoradiography. 125I-VEGF enhanced 3H-thymidine incorporation in umbilical vein endothelial cells and thus remained bioactive.
Carrier free human vegf165
Manufactured by R&D Systems
Carrier-free human VEGF165 is a recombinant human vascular endothelial growth factor isoform 165. It is provided in a carrier-free formulation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using carrier free human vegf165
1
Radiolabeling and Bioactivity of VEGF
2
Radiolabeling and Bioactivity of VEGF
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