Cell-free culture supernatants were collected and stored in deep-well plates at −30°C, until required. IFNγ and GzymB concentrations in culture supernatant were measured using ELISA. IFNγ assays used commercially available paired Abs and recombinant cytokines (BD Pharmingen; limit of detection = 3.91 pg/ml). GzymB assays used a commercially available ELISA kit (R&D Systems; limit of detection = 39.1 pg/ml), according to the manufacturer’s instructions.
Abs and recombinant cytokines
Manufactured by BD
Abs and recombinant cytokines are laboratory reagents used in various research and diagnostic applications. These products include antibodies and cytokines, which are proteins involved in cellular signaling and immune response. The core function of these products is to enable researchers to study and analyze biological processes at the cellular and molecular level. However, a detailed description of their specific applications and intended uses is not provided, as that would require more context and interpretation beyond the scope of this request.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using abs and recombinant cytokines
1
Quantifying Cytokine Secretion in Cell-free Supernatants
2
Autophagy Regulation in Immune Cells
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All standard chemicals and reagents used in the study were purchased from Sigma (St. Louis, MO) and Abs and recombinant cytokines from BD Biosciences (San Diego, CA), unless and otherwise mentioned. TLR-4 ligand (LPS) and NOD-2 ligand (N-glycolyl MDP) were procured from Invivogen (San Diego, CA). Anti-mouse LC-3 Ab was obtained from Sigma (St. Louis, MO).
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