Foxp3 pe cy7 fjk 16s

Manufactured by Thermo Fisher Scientific

The FoxP3 PE-Cy7 (FJK-16s) is a flow cytometry reagent used for the detection and analysis of FoxP3-expressing cells. FoxP3 is a transcription factor that is a marker for regulatory T cells. The FoxP3 PE-Cy7 (FJK-16s) reagent allows for the identification and quantification of FoxP3-positive cells in samples through flow cytometry.

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Lab products found in correlation

Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) F4 80 pe cy7 bm8, by BioLegend (1 mentions) Cd64 pe x54 5 7.1, by BioLegend (1 mentions) Mhc 2 pe m5 114.15.2, by Thermo Fisher Scientific (1 mentions) Ly 6g pe cy7 1a8, by BioLegend (1 mentions) Foxp3 staining buffer, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by Thermo Fisher Scientific (1 mentions) Plato 1, by Enzo Life Sciences (1 mentions) Cd45 bv510, by BioLegend (1 mentions) Ox40 pe ox 86, by BioLegend (1 mentions) Ghost red 780 viability dye, by Cytek Biosciences (1 mentions) Cd11b v450 m1 70, by BD (1 mentions) Cpg 1826, by Integrated DNA Technologies (1 mentions)

Close spelling variants of this product

FJK-16s (166 citations) Foxp3-PE (109 citations) Foxp3 (FJK-16s, (98 citations) Foxp3 PE-Cy7 (19 citations) Foxp3 PE (FJK-16s) (6 citations) Anti-mouse FOXP3 PE-Cy7 (FJK-16s, (2 citations)

4 protocols using foxp3 pe cy7 fjk 16s

Multicolor Flow Cytometry Analysis of Immune Cell Populations

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Anti-GD2 (clone 14.g2a) mAb was purified and filter sterilized in PBS. Total mouse IgG control Ab was obtained from Jackson Immunoresearch. Directly labeled mAbs used for staining were CD11b-A700 (M1/70), H-2K^b/H-2D^b-PE (28-8-2006), F4/80-PE-Cy7 (BM8), CD11c-PerCP (N418), CD4-PerCP (L3T4), Ly-6G-PE-Cy7 (1A8), CD64-PE (X54-5/7.1), CD16/CD32-A647 (2.4G2), CD206-APC (MR5D3) from Biolegend, CD45.2-FITC (104), CD11c-APC (HL3), NK1.1-PE (PK136), Ly-6C-PE (AL-21) and CD3-APC (145-2C11) from BD, MHC II-PE (M5/114.15.2) and FoxP3-PE-Cy7 (FJK-16s) from eBioscience, CD8-A700 (53–6.7) from Exbio. Cells for staining were washed in PBS, incubated with Viability Dye eFluor 780 (eBioscience), resuspended in PBA, transferred to a V-bottom 96-wells plate and stained using specific mAb or the appropriate isotypes. Cells analyzed on a Cyan apparatus (Beckman Coulter) using Summit software.

Kroesen M., Büll C., Gielen P.R., Brok I.C., Armandari I., Wassink M., Looman M.W., Boon L., den Brok M.H., Hoogerbrugge P.M, & Adema G.J. (2016). Anti-GD2 mAb and Vorinostat synergize in the treatment of neuroblastoma. Oncoimmunology, 5(6), e1164919.

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Quantifying Tumor-Associated T-Helper Cells

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B78 tumor-bearing mice were randomized into no treatment or RT groups when tumors were ~150 mm³ (n=5 per group). On day 5 after RT, mice were euthanized, and spleens were harvested and disaggregated between two microscope slides. Cells were prepped, Fc blocked, and stained for live/dead as described previously. Each sample was stained with the following antibodies in addition to those listed previously: CD3PE-Cy5 (145–2 C11) and CD122 PE (TM-β1) all from BioLegend and CD25 BB515 (PC61) from BD Biosciences. Samples were fixed and permeated overnight at 4°C with the FoxP3/Transcription Factor Staining Buffer Set from eBioscience following kit instructions. Samples were then stained with FoxP3 PE-Cy7 (FJK-16s) from eBioscience for 30 min at 4°C. Data were collected and analyzed as described previously for blood analysis.
CD4+ T cells with Tregs (CD45+ CD4+ CD25+ FoxP3+) gated out are referred to as ‘CD4+ T-helper cells’ (acknowledging that this population can contain other non-Treg CD4+ cells).

Pieper A.A., Rakhmilevich A.L., Spiegelman D.V., Patel R.B., Birstler J., Jin W.J., Carlson P.M., Charych D.H., Hank J.A., Erbe A.K., Overwijk W.W., Morris Z.S, & Sondel P.M. (2021). Combination of radiation therapy, bempegaldesleukin, and checkpoint blockade eradicates advanced solid tumors and metastases in mice. Journal for Immunotherapy of Cancer, 9(6), e002715.

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Treg Differentiation Assay in Cats

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CD4+ cells were purified from the clinically healthy, unrelated cat by MoFlo Cell Sorter, stained with CellTrace Violet, and added to a 96-well polystyrene plate at 2×10⁵ cells/well with about 4×10⁴ purified DC from FIV infected and control study cats. After 6 days in culture at 37°C, 5% CO₂, cells were stained for CD25-FITC [21 (link)] and GARP (Plato-1; Enzo, NY) for 20 min, washed with PBS, stained with IgG2b-PE (Jackson ImmunoResearch) for 20 min, then washed and permeablized by eBioscience FOXP3 staining buffer for 25 min. After washing twice, cells were stained with FOXP3-PE-Cy7 (FJK-16s; eBioscience, San Diego, CA) and Helios-APC (22F6; eBioscience) for 1 hr and were analyzed on an LSR II flow cytometer.

Krejci E., Duval N., Chatonnet A., Vincens P, & Massoulié J. (1991). Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid.. Proceedings of the National Academy of Sciences of the United States of America, 88(15), 6647-6651.

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In Vivo Immunotherapy Combination Protocol

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CpG 1826 was purchased from Integrated DNA Technologies. OX40 antibody [Anti-OX40 (CD134) antibody, rat immunoglobulin G1, OX86 clone, European Collection of Cell Cultures] was harvested and isolated from the ascites of immunodeficient mice, as previously described (33 (link)). CpG (50 µg) and/or OX40 (4 µg, 20 µg, or 100 µg) were injected IT with a 29 ½ gauge insulin syringe in 60 µL PBS every other day for three total doses (days 0, 2, 4 or days 5, 7, 9 depending on the experiment).
The following antibodies were used for flow cytometry analysis: anti-CD16/32 (93), CD45 BV510 (30-F11), CD45 FITC (30-F11), CD3 PE-Cy5 (145-2C11), CD4 BV785 (GK1.5), CD19 PE-Cy5 (6D5), CD19 APC (6D5), CD19 BV421 (6D5), Ly6G Alexa647 (1A8), IFNγ PE-Cy7 (XMG1.2), and OX40 PE (OX-86) all from BioLegend; CD8 APC-R700 (53-6.7), CD25 BB515 (PC61), NK1.1 PE-CF594 (PK136), Ly6C BV605 (AL-21), and CD11b V450 (M1/70) all from BD Biosciences; FoxP3/Transcription Factor Staining Buffer Set and FoxP3 PE-Cy7 (FJK-16s) from eBioscience. GhostRed780 Viability Dye (Tonbo Biosciences) was used for live/dead staining.

Pieper A.A., Zangl L.M., Speigelman D.V., Feils A.S., Hoefges A., Jagodinsky J.C., Felder M.A., Tsarovsky N.W., Arthur I.S., Brown R.J., Birstler J., Le T., Carlson P.M., Bates A.M., Hank J.A., Rakhmilevich A.L., Erbe A.K., Sondel P.M., Patel R.B, & Morris Z.S. (2021). Radiation Augments the Local Anti-Tumor Effect of In Situ Vaccine With CpG-Oligodeoxynucleotides and Anti-OX40 in Immunologically Cold Tumor Models. Frontiers in Immunology, 12, 763888.

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