Mouse trustain fcx antibodies

To test the percentage of neutrophil recruitment in skin and colon tissue from mice, flow cytometry acquisition was performed with the FACSVerse (BD Biosciences) and the stained cells were analyzed with flow cytometry software. First, single‐cell suspensions were obtained from skin and colon of balb/c nude mice, and incubated with mouse TruStain fcX Antibodies (Cat#101 319, Biolegend) for 10 min at 4 °C prior to immunostaining. Then cells were stained with fluorescently labeled antibodies with 1:100 dilutions including PerCP/Cyanine5.5 anti‐mouse Ly6G Antibody (Cat#127 615, BioLegend), APC anti‐mouse/human CD11b Antibody (Cat#101 211, BioLegend) and PE anti‐mouse CD45 Antibody (Cat#103 105, BioLegend).

Peripheral blood and human lymphocytes from the spleen were measured by a FACS Aria II flow cytometer (BD Biosciences, San Jose, CA). Cells were blocked using Human TruStain FcX and Mouse TruStain fcX Antibodies (Cat# 422302 and 101320, respectively, BioLegend) prior to staining. Then, cells were stained using antibodies against hCD45-APC/Cy7, hCD3-FITC, hCD4-PE/Cy7, hHLA-DR-APC, and hCD38-PE (Cat#304014, 300406, 344612, 307610, and 356604, respectively, BioLegend) as well as hCD8a-PE/Cy5.5 (Cat# 9536-16, Southern Biotech). Data were analyzed with FlowJo (version 10.0, FlowJo LLC, Ashland, OR).