Neopterin

Manufactured by Merck Group

Sourced in United States

Neopterin is a laboratory test kit developed by Merck Group. It is used to measure levels of neopterin, a biochemical marker that can provide information about the activation of the immune system. The test kit is designed to assist healthcare professionals in the clinical assessment and monitoring of various medical conditions. Neopterin levels can be determined using this product.

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Lab products found in correlation

4 protocols using neopterin

Nucleotide Synthesis and Purification

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Reagents, salts and buffers were of the highest quality grade, and were purchased from Sigma unless otherwise noted. Millipore ultrapure water (18.2 MΩ) was used for all solutions. [8-¹⁴C]-GTP was purchased from Moravek. Ultra pure grade 2′-dGTP and GTP were from Ameresco or Sigma. 8-oxo-GTP was purchased from Jena Bioscience. 7-deaza-GTP was purchased from Trilink Biotechnologies. Neopterin and glutathione were purchased from Sigma.

Paranagama N., Bonnett S.A., Alvarez J., Luthra A., Stec B., Gustafson A., Iwata-Reuyl D, & Swairjo M.A. (2017). Mechanism and catalytic strategy of the prokaryotic specific GTP cyclohydrolase IB. The Biochemical journal, 474(6), 1017-1039.

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Tryptophan Catabolism Metabolites Profiling

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Standard solutions for acylcarnitine and amino acid profiling, methanol, formic acid, bovine serum albumin (BSA), sodium chloride, 6-hydroxynicotinic acid, 3-indole acrylic acid, neopterin, biopterin, l-tryptophan, and ascorbic acid were received from Sigma–Aldrich (USA). Acetonitrile was obtained from Chromasolv^® (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland). Ultra-pure water was obtained from a Millipore Milli-Q water purification system (Millipore Corporation, Billerica, MA, USA). Isotope-labeled standard solutions of metabolites related to tryptophan catabolism were received from Toronto Research Chemicals (Toronto, ON, Canada). Isotope-labeled standard solutions for acylcarnitine and amino acid profiling were purchased from MassChrom Amino Acids and Acylcarnitines Non-Derivatised 57,000 Kit (Chromsystems, Germany).

Moskaleva N.E., Shestakova K.M., Kukharenko A.V., Markin P.A., Kozhevnikova M.V., Korobkova E.O., Brito A., Baskhanova S.N., Mesonzhnik N.V., Belenkov Y.N., Pyatigorskaya N.V., Tobolkina E., Rudaz S, & Appolonova S.A. (2022). Target Metabolome Profiling-Based Machine Learning as a Diagnostic Approach for Cardiovascular Diseases in Adults. Metabolites, 12(12), 1185.

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Metabolite Profiling for Amino Acids

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Standard solutions for amino acid, tryptophan metabolism intermediates and acylcarnitine profiling, as well as methanol, formic acid, bovine serum albumin (BSA), sodium chloride, 6-hydroxy nicotinic acid, 3-indole acrylic acid, neopterin, biopterin, 1-tryptophan, and ascorbic acid were received from Sigma‒Aldrich (USA). Acetonitrile was received from Chromasolv® (Sigma‒Aldrich Chemie GmbH, Buchs, Switzerland). Ultrapure water was received through the Millipore Milli-Q purification system (Millipore Corporation, Billerica, MA). Isotope-labeled standard solutions for tryptophan metabolism intermediate profiling were received from Toronto Research Chemicals (USA). Isotope-labeled standard solutions for metabolomic profiling were received from the MassChrom Amino Acids and Acylcarnitines Non Derivatized 57,000 Kit (Chromsystems, Germany).

Shestakova K.M., Moskaleva N.E., Boldin A.A., Rezvanov P.M., Shestopalov A.V., Rumyantsev S.A., Zlatnik E.Y., Novikova I.A., Sagakyants A.B., Timofeeva S.V., Simonov Y., Baskhanova S.N., Tobolkina E., Rudaz S, & Appolonova S.A. (2023). Targeted metabolomic profiling as a tool for diagnostics of patients with non-small-cell lung cancer. Scientific Reports, 13, 11072.

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Neopterin and Creatinine Quantification

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Neopterin (purity ≥97.5%), creatinine (purity ≥98.0%), dihydrogen potassium orthophosphate and methanol were purchased from Sigma (St. Louis, MO, USA). All chemicals used in the study were of quality analytical grade. Distilled water was prepared by an ultra-water purification system.

Yilmaz B., Yasar H., Asci A, & Kadioglu Y. (2022). Determination of neopterin in urine of industrial workers by HPLC. Anais da Academia Brasileira de Ciencias, 94(suppl 4).

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