Ni nta

Manufactured by Sangon

Sourced in China

Ni-NTA (Nickel-Nitrilotriacetic Acid) is a type of affinity chromatography resin used for the purification of recombinant proteins containing a polyhistidine (His-tag) sequence. It utilizes the strong interaction between the nickel (Ni2+) ions and the histidine residues on the target protein to selectively bind and capture the protein of interest.

Automatically generated - may contain errors

Lab products found in correlation

High fidelity thermostable dna polymerase, by Takara Bio (1 mentions) Bl21 de3, by Transgene (1 mentions) T4 dna ligase, by Takara Bio (1 mentions) Pmd18 t vector, by Takara Bio (1 mentions) Nanodrop 2000, by Thermo Fisher Scientific (1 mentions) Chemiluminescent emsa kit, by Beyotime (1 mentions)

Close spelling variants of this product

Ni-NTA agarose Ni-NTA resin Ni-NTA His Bind Resin Ni–NTA-Sefinose resin kit Ni-NTA chromatography Ni-NTA column Ni-NTA protein purification kit Ni-NTA-agarose columns Ni-NTA beads Ni-NTA 6FF

4 protocols using ni nta

Cloning and Expression of SUMO Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

E. coli competent cells DH5α and BL21 (DE3) were purchased from TransGen Biotech (Beijing, China). Expression vector pET28a-SUMO was purchased from Miaoling Biotech (Wuhan, China). Cloning vector pMD18-T vector was purchased from TaKaRa (Dalian, China). The restriction enzymes BamH Ⅰ and Hind Ⅲ, T4 DNA ligase and the high-fidelity thermostable DNA polymerase were from TaKaRa.
Primers were synthesized by Shanghai Sangon (China). Immobilized metal ion affinity chromatography Ni-NTA (Pre-Packed Gravity Column) was from Shanghai Sangon (China).

Ma W., Li X., Wang Q., Ren Z., Crabbe M.J.C, & Wang L. (2019). Tandem oligomeric expression of metallothionein enhance heavy metal tolerance and bioaccumulation in Escherichia coli. Ecotoxicology and environmental safety, 181.

+ Open protocol

+ Expand

PGRP-LB Protein Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

BL21 colonies were incubated in fresh LB medium at 37 °C with shaking at 200 rpm until OD 600 = 0.6. Then 0.5 mM isopropyl-β-dithiogalactoside was added to induce PGRP-LB expression with poly-His tags at the C-terminus. After incubation at 200 rpm for 4 h, the cells were collected and resuspended in Buffer B (8 M urea, 50 mM Tris-HCl, and 300 mM NaCl; pH 8.0). After sonication on ice for 10 min (5 s on, 10 s off), the supernatant was collected by centrifugation at 12,000×g and 4 °C for 10 min, mixed with nickel nitrilotriacetate (Ni-NTA; Sangon Biotech, Shanghai, China) and gently shaken at 4 °C overnight. The bound protein was eluted in PBS-T buffer with 20, 50, and 100 mM imidazole.

Yu Y., Luo C., Zhang D., & Chen J. (2021). Characterization of PGRP-LB and immune deficiency in the white-backed planthopper Sogatella furcifera (Hemiptera: Delphacidae). Applied Entomology and Zoology, 57(1), 1-14.

+ Open protocol

+ Expand

Recombinant Expression and Purification of PfIL-17A/F Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ORF sequence of Pf_IL-17A/F1, Pf_IL-17A/F2 and Pf_IL-17A/F3 genes after removal of the signal peptide sequence was amplified with gene-specific primers (Table 1) for prokaryotic expression. After digested with Sal I and EcoR I, the amplicon was inserted into the multiple cloning sites (MCS) of the pET-32a vector. The recombinant plasmid pET-32a-IL-17A/F1, pET-32a-IL-17A/F2 and pET-32a-IL-17A/F3 were then transformed into E. coli BL21 (DE3) competent cells, respectively, and the recombinant IL-17A/F (rIL-17A/F) expression was induced at 37°C for 4 h with IPTG at a final concentration of 1.0 mM. Subsequently, the rPf_IL-17A/F protein was purified by Ni-NTA (nitrilotriacetic acid) affinity chromatography (Sangon, Shanghai, China) according to the manufacturer’s instructions. The identity of the recombinant protein was confirmed by SDS-PAGE as a band with the correct molecular weight. The purified protein was quantitated using the Bradford protein quantitation assay by Nanodrop 2000 (Thermo Electron Corporation, USA).

Zhou X., Zhang G.R., Ji W., Shi Z.C., Ma X.F., Luo Z.L, & Wei K.J. (2021). Expression and Function Analysis of Interleukin-17A/F1, 2, and 3 Genes in Yellow Catfish (Pelteobagrus fulvidraco): Distinct Bioactivity of Recombinant IL-17A/F1, 2, and 3. Frontiers in Immunology, 12, 626895.

+ Open protocol

+ Expand

Purification and EMSA of CmAreA Transcription Factor

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cDNA sequence of Cmarea was cloned into vector PET28a (provided by Prof. Linqi Wang from the Institute of Microbiology, Chinese Academy of Sciences) with a 6×His-tag and introduced into E. coli BL21-DE3 (Biomed Biotech Co., Ltd., Beijing, China). Expressed CmAreA was purified using nickel–nitrilotriacetic acid (Ni-NTA, Sangon Biotech Co., Ltd., Shanghai, China).
A 50 bp DNA probe containing the GATA sequences from the Cmhyd1 promoter was labeled with digoxigenin-11-ddUTP (DIG-11-ddUTP, Beyotime Biotech Co., Ltd., Beijing, China) at the 3′ end (Table S1). EMSA was performed with a Chemiluminescent EMSA Kit (Beyotime Biotech Co., Ltd., Beijing, China), following the manual.

Li X., Wang F., Liu M, & Dong C. (2021). Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus, Cordyceps militaris. Journal of Fungi, 7(8), 674.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!