The iPSC-ECs were cultured in 96-well plates. Cell analyses were performed using a CCK-8-based in vitro cell proliferation and cytotoxicity assay kit (Beyotime) according to the manufacturer's instructions. Cells were incubated with 10 μL of CCK-8 reagent per well for 12 h. Absorbance at 450 nm was measured using an iMark microplate reader (Bio-Rad).
Cck 8 based in vitro cell proliferation and cytotoxicity assay kit
Manufactured by Beyotime
The CCK-8-based in vitro cell proliferation and cytotoxicity assay kit is a colorimetric assay for the determination of cell proliferation and cytotoxicity. The kit utilizes the water-soluble tetrazolium salt WST-8 to produce a water-soluble formazan dye, which can be quantified by measuring the absorbance at 450 nm.
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Lab products found in correlation
3 protocols using cck 8 based in vitro cell proliferation and cytotoxicity assay kit
1
Cell Proliferation and Cytotoxicity Assay
2
Cell Viability Assay using CCK8
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H9-ECs were cultured in 96-well plate and cell viability analyses were performed using CCK8-based in vitro cell proliferation and cytotoxicity assay kit (Beyotime) according to the manufacturer’s instructions. Cells were incubated in the presence of 10 μl CCK8 reagent per well for 3 h. Absorbance at 450 nm was measured using a MD M5 SpectraMax reader (Molecular Devices).
3
Cell Viability Analysis of H9-CMs
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H9‐CMs were cultured in 96‐well plate. Cell viability analyses were performed using CCK8 based in vitro cell proliferation and cytotoxicity assay kit (Beyotime) according to the manufacturer′s instructions. Cells were incubated in the presence of 10 μL CCK8 reagent per well for 3 hours. Absorbance at 450 nm was measured using an iMark™ microplate reader (Bio‐Rad).
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