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Fetal bovine serum (fbs)

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Fetal bovine serum is a cell culture supplement derived from the blood of bovine fetuses. It provides a complex mixture of proteins, growth factors, and other components that support the growth and maintenance of a variety of cell types in vitro.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Ifn α2a, by PBL Assay Science (1 mentions) Z vad fmk, by Apexbio (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Nh4cl, by Merck Group (1 mentions) Poly 1 c lmw, by InvivoGen (1 mentions) Recombinant human ifn β, by Thermo Fisher Scientific (1 mentions) Poly da dt, by InvivoGen (1 mentions) Dulbecco s modified eagle medium, by Corning (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Raw 264, by Lonza (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Lonza (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions) Dmem medium, by Lonza (1 mentions)

4 protocols using fetal bovine serum (fbs)

1

Cell Culture Conditions for HEK 293T, HeLa, and BMDC

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HEK 293T and HeLa cells were cultured in DMEM (HyClone) with 10% FBS (GenStar) and 1% l-glutamine (Gibco). BMDCs were cultured in RPMI-1640 medium (Gibco) with 10% FBS (GenStar) and 1% l-glutamine (Gibco). Cells were incubated at 37 °C in 5% CO2.
Liu D., Zhao Z., She Y., Zhang L., Chen X., Ma L, & Cui J. (2022). TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation. Proceedings of the National Academy of Sciences of the United States of America, 119(7), e2113454119.
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2

Cell Culture and Transfection Methods

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HEK-293T cells and HeLa cells were cultured in Dulbecco's modified Eagle's medium (Gibco) supplemented with 10% fetal bovine serum (GenStar) and antibiotics. Vero E6 cells and A549 cells were cultured in Dulbecco's modified Eagle's medium (Gibco) supplemented with 10% fetal bovine serum (Gibco). Transfection of cells was performed using Lipofectamine 3000 (Life Technologies) in Opti-MEM (Life Technologies) according to the manufacturer’s instructions. Cells were treated with IFN-α2a (catalog no. 11101–2; PBL Assay Science) at 1000 units/ml, MG132 (MCE) at 20 μM, Z-VAD-FMK (APE x BIO) at 20 μM, and NH4Cl (sigma-Aldrich) at 10 mM.
Song L., Wang D., Abbas G., Li M., Cui M., Wang J., Lin Z, & Zhang X.E. (2023). The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. The Journal of Biological Chemistry, 299(3), 102990.
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3

Establishment of Cell Culture Conditions

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HEK293T, A549, HT1080, HeLa, LN229, U87 and U251 cells were cultured in Dulbecco’s modified Eagle medium (Corning, Manassas, VA, USA) with 10% fetal bovine serum (Genstar, Beijing, China) in a 5% CO2 incubator at 37 °C. Recombinant human IFNβ was purchased from Peprotech (Rocky Hill, NJ, USA). Poly (I:C) (LMW) and poly (dA:dT) were purchased from Invivogen (San Diego, CA, USA). Doxycycline (D9891), MG132 (C-2211-5MG), Bafilomycin A1 (H2714) and 3-methyladenine (M9281-100MG) were purchase from Sigma (Shanghai, China).
Wu Y., Liu Q., Zhou J., Xie W., Chen C., Wang Z., Yang H, & Cui J. (2017). Zika virus evades interferon-mediated antiviral response through the co-operation of multiple nonstructural proteins in vitro. Cell Discovery, 3, 17006-.
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4

Cell Culture Protocols for THP-1, RAW264.7, and HEK293T

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The human myelomonocytic cell line THP‐1 (ATCC TIB‐202) was cultured in RPMI 1640 medium (LONZA) supplemented with 10% FBS (GIBCO) at 37°C in a 5% CO2 humidified incubator. The mouse macrophage cell line RAW264.7 (Laboratory storage) was maintained in DMEM (LONZA) supplemented with 10% FBS (GIBCO) at 37°C in a 5% CO2 humidified incubator. THP‐1 cells were differentiated using 25 ng/ml phorbol myristate acetate (PMA; Sigma‐Aldrich) for 48 hr. Then, the medium was replaced, and the cells were cultured in fresh RPMI 1640 medium supplemented with 10% FBS and incubated for an additional 12 hr for cell differentiation. HEK293T (Laboratory storage) cells were cultured in DMEM medium (LONZA) supplemented with 10% FBS (GenStar).
Wei S., Wang D., Li H., Bi L., Deng J., Zhu G., Zhang J., Li C., Li M., Fang Y., Zhang G., Chen J., Tao S, & Zhang X. (2019). Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF‐κB signalling pathway by binding to eEF1A1. Cellular Microbiology, 21(12), e13090.
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