Il 10

Manufactured by MultiSciences Biotech

Sourced in China, United States

IL-10 is a recombinant protein that is commonly used in laboratory research. It functions as an anti-inflammatory cytokine and plays a role in the regulation of the immune system.

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Lab products found in correlation

Interleukin 6 (il 6), by MultiSciences Biotech (9 mentions) Il 1β, by MultiSciences Biotech (8 mentions) Tnf α, by MultiSciences Biotech (7 mentions) Interleukin 4 (il 4), by MultiSciences Biotech (3 mentions) Elisa kit of tnf α, by MultiSciences Biotech (3 mentions) Tgf β, by MultiSciences Biotech (3 mentions) Ifn γ, by R&D Systems (2 mentions) Il 17a, by MultiSciences Biotech (2 mentions) Brain derived neurotrophic factor (bdnf), by Promega (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Ifn γ and il 2 elisa kits, by Dakewe (1 mentions) Synergy 2, by Agilent Technologies (1 mentions) Thrombin, by Elabscience (1 mentions) Adiponectin, by Merck Group (1 mentions) Cholesterol, by Roche (1 mentions) Leptin, by R&D Systems (1 mentions) Ifn β, by Cusabio (1 mentions) Microplate reader, by Thermo Fisher Scientific (1 mentions) Hydroxyproline assay kit, by Nanjing Jiancheng (1 mentions) Matrine, by Yuanye Bio-Technology (1 mentions)

Spelling variants of this product

Human IL-10 ELISA kit (4 citations) Rat IL-10 ELISA Kit (2 citations) Human IL-10 (2 citations) IL-10 ELISA kit (2 citations)

18 protocols using il 10

Cytokine Measurement in Tumor Mice

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For the cytokine assay, mouse serum was harvested from orthotopic tumor-bearing mice and centrifuged at 3000 g for 30 min at room temperature, followed by measurement of IFN-γ (R&D systems, US) and IL-10 (MultiSciences Biotech Co., Ltd., China), respectively.

Rao Q., You A., Guo Z., Zuo B., Gao X., Zhang T., Du Z., Wu C, & Yin H. (2016). Intrahepatic Tissue Implantation Represents a Favorable Approach for Establishing Orthotopic Transplantation Hepatocellular Carcinoma Mouse Models. PLoS ONE, 11(1), e0148263.

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Measurement of Neuroinflammatory Markers

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The mentioned culture supernatants yielded here were collected to cytokine concentrations of IL-1β, IL-4, IL-6, IL-10, TNF (Multisciences, China), and the secretion of neurotrophic factor BDNF (Promega, USA) in the brain by a sandwich ELISA kits in line with the instructions of the manufacturer. The determinations were conducted in duplicate. The results are denoted as pg/mL.

Tang Y., Han L., Bai X., Liang X., Zhao J., Huang F, & Wang J. (2020). Intranasal Delivery of Bone Marrow Stromal Cells Preconditioned with Fasudil to Treat a Mouse Model of Parkinson’s Disease. Neuropsychiatric Disease and Treatment, 16, 249-262.

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Synthesis and Characterization of SPRC Nanoparticles

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SPRC was synthesized as we reported previously (Wang et al. 2009 (link)). Tetraethyl orthosilicate (TEOS) was purchased from Aladdin Reagent Co., Ltd (Shanghai, China). Cetyltrimethylammonium chloride (CTAC) solution (25 wt % in H₂O), 3-aminopropyltriethoxysilane (APTES), triethanolamine (TEA) and decahydronaphthalene (DHA) were all purchased from Sigma Aldrich (St Louis, USA). Complete Freund’s Adjuvant (CFA), propargylglycine (PAG), BMDM medium (Iscove’s modified Dulbecco’s medium supplemented with 10% fetal calf serum, 100 μ mL⁻¹ penicillin, 100 μg mL⁻¹ streptomycin, 10 mM thioglycerol), human macrophage-colony stimulating factor (M-CSF), sodium hydroxide, hydrochloric acid, acetonitrile, methanol, toluene, anhydrous ethanol, sodium sulfide, monobromobimane (MBB), diethylenetriaminepentaacetic acid (DTPA), sulfosalicylic acid and hydrocortisone were purchased from Macklin Industrial Corporation (Shanghai, China). Elisa kit of TNF-α, IL-1β, IL-6 and IL-10 were purchased from MultiSciences (Hangzhou, China).

Yu Y., Wang Z., Yang Q., Ding Q., Wang R., Li Z., Fang Y., Liao J., Qi W., Chen K., Li M, & Zhu Y.Z. (2021). A novel dendritic mesoporous silica based sustained hydrogen sulfide donor for the alleviation of adjuvant-induced inflammation in rats. Drug Delivery, 28(1), 1031-1042.

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Cytokine Analysis of Lentiviral Vaccine Therapy

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Blood (centrifuged at 3000 g for 30 min at room temperature) and tumor homogenates (150 mg tumor tissue) were harvested from Lenti-AFP-, Lenti-HA-treated or control HCC mice and followed by analysis of IFN-γ (R&D systems, US), IL-2 (eBioscience, US), IL-10 (MultiSciences Biotech Co., Ltd., China) and TGF-β kits (MultiSciences Biotech Co., Ltd., China), respectively. To examine the immune response in orthtopic HCC mice treated with repeated administrations of lentiviral vaccines in a real-time manner, we collected blood 3 days after each injection via retro-orbital bleeding. For murine in vitro study, IFN-γ and IL-2 ELISA kits were purchased from R&D systems and eBioscience (US). For human in vitro cytokine assay, IFN-γ and IL-2 ELISA kits were purchased from Dakewe Biotech Co., Ltd (China). Briefly, 1x10⁵ activated BMDCs (transduced with lentivirus), inguinal lymph node CD11c⁺DC or peripheral monocyte-derived DCs (PMDCs) were incubated with 1x10⁶ mixed splenocytes from naïve mice or human lymphocytes for 72 hrs. Levels of IFN-γ and IL-2 in supernatant were detected as per the manufacturer's instructions.

Liu Z., Lu Z., Jing R., Zuo B., Gao X., Han G., Qi H., Wu L., Liu Y, & Yin H. (2019). Alarmin augments the antitumor immunity of lentiviral vaccine in ectopic, orthotopic and autochthonous hepatocellular carcinoma mice. Theranostics, 9(14), 4006-4018.

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Cytokine Profiling in Cell Samples

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Protein samples were prepared using the extraction buffer supplemented with a protease inhibitor PMSF as mentioned above. Homogenate was centrifuged at 12,000 rpm for 15 min at 4 °C, and the supernatant was collected for TNF-α, IL-1β, IL-6, IL-4, IL-10 (MULTI SCIENCES), and thrombin (Elabscience) ELISA assay. The contents of the cytokines were expressed as pg/ml/10⁵ cells for the supernatant, and pg/mg for the lysate of the cells and the cord tissues. Plates were read with a multifunctional enzyme marker (Biotek Synergy2) at a 450-nm wavelength.

Chen X., Zhang H., Hao H., Zhang X., Song H., He B., Wang Y., Zhou Y., Zhu Z., Hu Y, & Wang Y. (2022). Thrombin induces morphological and inflammatory astrocytic responses via activation of PAR1 receptor. Cell Death Discovery, 8, 189.

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Measuring Metabolic Markers in Lean and DIO Mice

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We utilised the corresponding ELISA or assay kits according to the manufacturers’ instructions to measure plasma leptin (R&D), Il10 (Multisciences), adiponectin (Millipore), cholesterol (Roche) and triglycerides (Roche). We used 5 μl of serum sample from lean mice and 5 μl of 5× diluted serum sample from mice with DIO for the leptin ELISA. We used 5 μl of serum from lean mice, mice with DIO for the insulin ELISA, 3 μl for the cholesterol assay and 3 μl for the triglyceride assay.

Chen J., Xu X., Li Y., Li F., Zhang J., Xu Q., Chen W., Wei Y, & Wang X. (2020). Kdm6a suppresses the alternative activation of macrophages and impairs energy expenditure in obesity. Cell Death and Differentiation, 28(5), 1688-1704.

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Quantifying Tumor Immunomodulatory Cytokines

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Levels of the cytokines IFN-α (ExCell Biology, China) and IFN-β (CUSABIO, China) in the culture supernatant of Hepa1-6 cells and in the serum of tumor-bearing mice were detected by Enzyme-linked immunosorbent assay (ELISA). Concentrations of the cytokines TGF-β, and IL-10 (Multi Sciences Biotech, China) in the serum or tumor tissue homogenate of tumor-bearing mice were also detected by ELISA. ELISAs for cytokine detection were performed according to the manufacturer’s instructions.

Shao L., Yu X., Han Q., Zhang X., Lu N, & Zhang C. (2022). Enhancing anti-tumor efficacy and immune memory by combining 3p-GPC-3 siRNA treatment with PD-1 blockade in hepatocellular carcinoma. Oncoimmunology, 11(1), 2010894.

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Serum Biomarker and Wound Healing Analysis

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The serum levels of total protein (TP), albumin (ALB), and prealbumin (PA) were measured using ELISA kits (Nanjing Jiancheng, China). The levels of inflammatory factors IL-6 (Multi Sciences, China), IL-8 (Sbjbio Scientific, Inc., China), and IL-10 (Multi Sciences, China) were also quantified using ELISA kits. All the assays were performed using a microplate reader (Thermo Fisher Scientific). The hydroxyproline concentration in the tissues around the wounds was determined using a hydroxyproline assay kit (Nanjing Jiancheng, China). The ELISA was performed according to the manufacturer's instructions.

Wang X., Yu Z., Zhou S., Shen S, & Chen W. (2022). The Effect of a Compound Protein on Wound Healing and Nutritional Status. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 4231516.

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Cytokine Profiling of Serum and Supernatant

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Samples of serum and supernatant were acquired, processed and immediately frozen at −80°C to await analysis. The level of cytokines in these samples was then determined using a range of commercial ELISA kits: TNF‐α, IL‐17A, TGF‐β and IL‐10 (Multi Sciences, Hangzhou, China); and IL‐35 (Bioswamp). All assays were carried out in accordance with the manufacturer's guidelines. ELISA data were detected using a Tecan Sunrise infinite M200 PRO platereader (Tecan, Switzerland).

Cheng H., Xu B., Zhang L., Wang Y., Chen M, & Chen S. (2021). Bortezomib alleviates antibody‐mediated rejection in kidney transplantation by facilitating Atg5 expression. Journal of Cellular and Molecular Medicine, 25(23), 10939-10949.

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Cytokine Profiling in Blood Samples

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The collected blood samples were isolated three times by centrifugation at 1500 × g for 10 minutes each. The supernatants were then analyzed using ELISA kits for tumor necrosis factor (TNF)-α (catalog no: 70-EK182-96), IL-1β, (catalog no: 70-EK101B-96), IL-6 (catalog no: 70-EK106/2-96), IL-7 (catalog no: 70-EK107-96), IL-10 (catalog no: 70-EK110/2-96), and IL-15 (catalog no: 70-EK115-96) (all MultiSciences, China), according to the manufacturer’s instructions. All assays were carried out in triplicate.

He J., Xu Y., Yi M., Gu C., Zhu Y, & Hu G. (2020). Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain. The Journal of International Medical Research, 48(7), 0300060519871407.

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