Some images were collected on an Axioplan 2 microscope (Zeiss) with 63x/1.4 NA objective (Zeiss) and dual-band filter set (Chroma, set 51019). Most images were collected on a DeltaVision Core imaging system at the Rockefeller Bioimaging Facility (GE Healthcare Life Sciences) with a with an Olympus IX-71 microscope and Insight 7 color SSI illumination system using an UPLSAPO 60x/1.3 NA Silicone oil objective (Olympus) and a pro.edge sCMOS camera. Images were acquired and deconvolved using measured PSFs using SoftWorx software (GE Healthcare Life Sciences). For still embryo imaging, embryos were anesthetized using 0.5 M sodium azide. Larvae were paralyzed as described above.
Uplsapo 60x 1.3 na silicone oil objective
Manufactured by Olympus
The UPLSAPO 60x/1.3 NA Silicone oil objective is a high-performance objective lens designed for advanced microscopy applications. It features a high numerical aperture of 1.3 and is optimized for use with silicone oil immersion. The lens provides excellent resolution and image quality, making it suitable for a variety of microscopy techniques.
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2 protocols using uplsapo 60x 1.3 na silicone oil objective
1
Deconvolution Microscopy of Embryos
2
Deconvolution Microscopy of Embryos
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Some images were collected on an Axioplan 2 microscope (Zeiss) with 63x/1.4 NA objective (Zeiss) and dual-band filter set (Chroma, set 51019). Most images were collected on a DeltaVision Core imaging system at the Rockefeller Bioimaging Facility (GE Healthcare Life Sciences) with a with an Olympus IX-71 microscope and Insight 7 color SSI illumination system using an UPLSAPO 60x/1.3 NA Silicone oil objective (Olympus) and a pro.edge sCMOS camera. Images were acquired and deconvolved using measured PSFs using SoftWorx software (GE Healthcare Life Sciences). For still embryo imaging, embryos were anesthetized using 0.5 M sodium azide. Larvae were paralyzed as described above.
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