All library preparations and sequencing were performed using Oxford Nanopore Ligation Sequencing Kits SQK-LSK108 and SQK-LSK109 according to the manufacturer’s instructions (Oxford Nanopore Technologies). For the SQK-LSK108 sequencing Kit, 90 μg of DNA was purified then sheared to 20-kb fragments using the megaruptor1 system (Diagenode). For each library, a DNA-damage repair step was performed on 5 μg of DNA. Then an end-repair-dA-tail step was performed for adapter ligation. Libraries were loaded onto nine R9.4.1 flowcells and sequenced on a GridION instrument at a concentration of 0.1 pmol for 48 h. For the SQK-LSK109 sequencing Kit, 10 μg of DNA was purified then sheared to 20-kb fragments using the megaruptor1 system (Diagenode). For this library, a one-step DNA-damage repair +end-repair-dA-tail procedure was performed on 2 μg of DNA. Adapters were then ligated to DNAs in the library. The library was loaded onto one R9.4.1 flowcell and sequenced on a GridION instrument at a concentration of 0.08 pmol for 48 h.
Ligation sequencing kits sqk lsk108
Manufactured by Oxford Nanopore
The Oxford Nanopore Ligation Sequencing Kits SQK-LSK108 are a set of reagents and consumables designed for use with Oxford Nanopore sequencing platforms. The kits enable the preparation of DNA or RNA samples for sequencing using the ligation-based approach.
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Lab products found in correlation
2 protocols using ligation sequencing kits sqk lsk108
1
Nanopore Sequencing of Long DNA Fragments
2
Nanopore Sequencing Library Preparation
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All library preparations and sequencing were performed using Oxford Nanopore Ligation Sequencing Kits SQK-LSK108 and SQK-LSK109 according to the manufacturer's instructions (Oxford Nanopore Technologies). For the SQK-LSK108 sequencing Kit, 90 µg of DNA was purified then sheared to 20 kb fragments using the megaruptor1 system (Diagenode). For each library, a DNAdamage repair step was performed on 5 µg of DNA. Then an END-repair-dA-tail step was performed for adapter ligation. Libraries were loaded onto nine R9.4.1 flowcells and sequenced on a GridION instrument at a concentration of 0.1 pmol for 48 h. For the SQK-LSK109 sequencing Kit, 10 µg of DNA was purified then sheared to 20 kb fragments using the megaruptor1 system (Diagenode). For this library, a one-step DNA-damage repair + END-repair-dA-tail procedure was performed on 2 µg of DNA. Adapters were then ligated to DNAs in the library. The library was loaded onto one R9.4.1 flowcell and sequenced on a GridION instrument at a concentration of 0.08 pmol for 48h.
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