Xyz3060 dispense platform

The colloidal gold-based lateral flow test strips were generated according to the procedures described by Liang et al. (2015) (link), with some modifications. Briefly, 25 × 300-mm pieces of glass fiber was immersed in phosphate-buffered saline (0.2 M, pH 8.0, containing 10 mM EDTA, 1 % Tween-20, and 10 % BSA) for 2 h at 20 °C. Next, the pieces were dried for 2 h at 37 °C and stored for use as the sample pad. Glass fiber was also immersed in phosphate-buffered saline (0.1 M, pH 7.4, containing 2.5 % mycose, 1 % BSA, 1 % Tween-20) for 2 h at 20 °C and then dried for 2 h at 37 °C and stored for use as the conjugate pad. The conjugate pad was covered with an appropriate volume of colloidal gold-labeled anti-SIgA SC mAbs (2F9) using a XYZ3060 dispense platform (BioDot, Inc., Irvine, CA, USA), and then dried at 37 °C for 2 h and stored dry. Purified PEDV particles were dispensed onto the nitrocellulose (NC) membrane to serve as the test line, and goat anti-mouse IgG polyclonal antibody (pAb) was dispensed onto the NC membrane, (being separated by a distance of 7 mm) to serve as the control line. The PEDV particles and goat anti-mouse IgG pAb were diluted with coating buffer to a final concentration of 0.02 and 1.0 mg/mL, respectively. Then, the NC membrane was dried at 37 °C for 2 h and stored in the dry at room temperature until use.