Anti mdm2 sc 813

Manufactured by Santa Cruz Biotechnology

Anti-MDM2 (sc-813) is a primary antibody that recognizes the MDM2 protein. MDM2 is a negative regulator of the p53 tumor suppressor protein. This antibody is designed for use in western blotting, immunoprecipitation, and immunofluorescence applications.

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Lab products found in correlation

Anti zeb1, by Cell Signaling Technology (1 mentions) Anti beta actin ab8227, by Abcam (1 mentions) Laminin l2020, by Merck Group (1 mentions) Anti nestin sc 23927, by Santa Cruz Biotechnology (1 mentions) Anti p53 sc6243, by Santa Cruz Biotechnology (1 mentions) Anti tubulin t5168, by Merck Group (1 mentions) Anti γh2ax ab26350, by Abcam (1 mentions) Anti flag f1804, by Merck Group (1 mentions) Anti k48 linked ubiquitin, by Cell Signaling Technology (1 mentions) Anti myc 11667149001, by Roche (1 mentions) Ab183499, by Abcam (1 mentions) Ab181140, by Abcam (1 mentions) Anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Anti gfp ab290, by Abcam (1 mentions) Anti β actin c4, by Santa Cruz Biotechnology (1 mentions) Odyssey clx imaging system, by LI COR (1 mentions) Anti usp7 a300 033a, by Fortis Life Sciences (1 mentions) Anti ubiquitin fk2, by Enzo Life Sciences (1 mentions) Rabbit anti mouse immunoglobulins hrp, by Agilent Technologies (1 mentions) Goat anti rabbit immunoglobulins hrp, by Agilent Technologies (1 mentions)

3 protocols using anti mdm2 sc 813

Antibody Validation and Cellular Markers

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AMG232 and RG7112 were purchased from APExBIO Technology. Anti-p21 (#2947), anti-PARP (#9542), and anti-ZEB1 (#3396) were purchased from Cell Signaling, anti-p53 (sc-6243), anti-MDM2 (sc-813), and anti-Nestin (sc-23927) were purchased from Santa Cruz Biotechnology and anti-beta-actin (ab8227) was purchased from Abcam. Alexa Fluor 488 anti-rabbit secondary antibody (A11008) was purchased from Thermo Fisher Scientific. Laminin (L2020) was purchased from Sigma and Hoechest33342 (H3570) was purchased from Life Technologies.

Her N.G., Oh J.W., Oh Y.J., Han S., Cho H.J., Lee Y., Ryu G.H, & Nam D.H. (2018). Potent effect of the MDM2 inhibitor AMG232 on suppression of glioblastoma stem cells. Cell Death & Disease, 9(8), 792.

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Antibody Reagents for Protein Analysis

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Anti-β-TrCP1 (sc-390629), anti-His (sc-8036), anti-HA (sc-7392), anti-Ubiquitin (sc-8017), anti-p53 (sc126), anti-ATM (sc-53173), anti-GST (sc-138), and anti-MDM2 (sc-813) were purchased from Santa Cruz Biotechnology. anti-pATM (13050) and anti-K63-linked ubiquitin (5621), anti-γH2AX (9718), and anti-K48-linked ubiquitin antibodies (8081) were purchased from Cell Signaling Technology. We purchased anti-β-TrCP2 (PA5-29878) from Thermo Fisher Scientific, anti-γH2AX (ab26350) from Abcam and anti-myc (11667149001) from Roche. Antibody against Cullin1 (100-401-A01) was procured from Rockland. We obtained anti-FLAG (F1804) and anti-Tubulin (T5168) antibodies from Sigma-Aldrich.

Islam S., Dutta P., Sahay O, & Santra M.K. (2021). β-TrCP1 facilitates cell cycle checkpoint activation, DNA repair, and cell survival through ablation of β-TrCP2 in response to genotoxic stress. The Journal of Biological Chemistry, 296, 100511.

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Comprehensive Western Blot Analysis

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Western blotting was performed as previously described (van den Boom et al., 2013 (link)). The following primary antibodies were used in this study: anti-KDM2B (09-864, Merck), anti-USP7 (A300-033A, Bethyl Laboratories), anti-TRIM27 (18791, IBL), anti-RING1A (ab180170, Abcam), anti-RING1B (ab181140, Abcam), anti-PCGF1 (ab183499, Abcam), anti-STAT5 (sc-835-G, Santa Cruz Biotechnology), anti-TP53 (sc-216, Santa Cruz Biotechnology), anti-MDM2 (sc-813, Santa Cruz Biotechnology), anti-GFP (sc-9996, Santa Cruz Biotechnology), anti-GFP (ab290, Abcam), anti-Ubiquitin (FK2, Enzo Life Sciences), anti-H2AK119ub (D27C4, Cell Signaling), and anti-b-Actin (C4, Santa Cruz Biotechnology). Secondary antibodies used included either goat anti-mouse IRDye 800 or goat anti-rabbit IgG (H+L) Alexa Fluor 680 (Invitrogen) for imaging using an Odyssey CLx Imaging System (Li-Cor Biosciences), or goat anti-rabbit immunoglobulins/HRP (Agilent-Dako), and rabbit anti-mouse immunoglobulins/HRP (Agilent-Dako) for imaging using a ChemiDoc XRS+ System (Biorad).

Maat H., Atsma T.J., Hogeling S.M., Rodríguez López A., Jaques J., Olthuis M., de Vries M.P., Gravesteijn C., Brouwers-Vos A.Z., van der Meer N., Datema S., Salzbrunn J., Huls G., Baas R., Martens J.H., van den Boom V, & Schuringa J.J. (2021). The USP7-TRIM27 axis mediates non-canonical PRC1.1 function and is a druggable target in leukemia. iScience, 24(5), 102435.

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