Animal use conformed to protocols approved by the Wesleyan University Institutional Animal Care and Use Committee. Experiments were performed using group-housed C57BL/6NHsd mice (Table 1 , Envigo) maintained in self-ventilating cages, under a 12 h light–dark cycle with light onset at 7 AM, room temperature of 25 deg. C, humidity of 17%–20%, and light intensity of 193–215 lx at the cage level. The mice were group-housed until they were 4–5 weeks of age. They were handled for 5 min per day for one week prior to the beginning of the experiment. The mice consumed food and water ad libitum. Donor embryos were generated by breeding VGAT-ChR2-eYFP transgenic males (Jackson Labs) with C57BL/6NHsd females (Envigo).
Vgat chr2 eyfp transgenic males
Manufactured by Jackson ImmunoResearch
VGAT-ChR2-eYFP transgenic males express the light-sensitive protein channelrhodopsin-2 (ChR2) fused to the enhanced yellow fluorescent protein (eYFP) under the control of the vesicular GABA transporter (VGAT) promoter. This allows for the visualization and optogenetic manipulation of GABAergic neurons.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vgat chr2 eyfp transgenic males
1
Standardized Murine Housing Protocol
2
Standardized Murine Housing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animal use conformed to protocols approved by the Wesleyan University Institutional Animal Care and Use Committee. Experiments were performed using group-housed C57BL/6NHsd mice (Table 1 , Envigo) maintained in self-ventilating cages, under a 12 h light–dark cycle with light onset at 7 AM, room temperature of 25 deg. C, humidity of 17%–20%, and light intensity of 193–215 lx at the cage level. The mice were group-housed until they were 4–5 weeks of age. They were handled for 5 min per day for one week prior to the beginning of the experiment. The mice consumed food and water ad libitum. Donor embryos were generated by breeding VGAT-ChR2-eYFP transgenic males (Jackson Labs) with C57BL/6NHsd females (Envigo).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!