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Dichloromethane

Manufactured by Beijing Chemical Works
Sourced in China

Dichloromethane is a colorless, volatile liquid commonly used as a solvent in laboratory settings. It has a low boiling point and is miscible with a wide range of organic solvents. Dichloromethane is a versatile laboratory reagent that can be used for various extraction and purification processes.

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12 protocols using dichloromethane

1

Synthesis of Functionalized Polymeric Nanocarriers

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Pluronic F127 and methane sulfonyl chloride were obtained from Sigma-Aldrich. Nε-Benzyloxycarbonyl-l-lysine (l-lysine(z)) was purchased from Energy Chemical. Polyethylene glycol (PEG4000), triethylamine, triphosgene, trifluoroacetic acid (TFA), 5-FU (98%), and trifluoroacetic acid-d (TFA-d) were obtained from Aladdin. Moreover, 33% HBr/acetic acid solution was acquired from J&K Chemical. Dichloromethane, tetrahydrofuran (THF), N,N-dimethylformamide (DMF), chloroform, petroleum ether, ammonium hydroxide solution, potassium dihydrogen phosphate, and sodium hydroxide were purchased from Beijing Chemical Works. Dimethyl sulfoxide-d6 (DMSO-d6) was obtained from CIL.
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2

Synthesis and Characterization of Photocatalysts

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Iron(II) chloride tetrhydrate (98%, Energy Chemical Ltd., Shanghai, China), 1,10-Phenanthroline (99%, J&K Scientific Ltd., Beijing, China), [Ru(bpy)3]Cl2·6H2O, 2-Phenylbenzimidazole (98%, J&K Scientific Ltd., Beijing, China), Iodomethane (97%, Ouhechem, Beijing, China), Sodium borohydride (99%, HuaDa, Guangdong, China). Sodium chloride, Sodium thiosulfate, Ethanol, Dichloromethane, Dithyl ether, N,N-Dimethylformamide, TriEthanolamine come from Beijing Chemical Works (Beijing, China), and were used as purchased, without further purification.
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3

Identification and Quantification of Volatile Compounds

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The internal standard (4-methyl-2-pentanol) and C6-C24 n-alkanes used for the identification and quantification of volatile compounds were purchased from Sigma-Aldrich (St. Louis, MO, USA). The methyl ester of long-chain fatty acids was purchased from Sigma-Aldrich and Fluka (Buchs, Switzerland). Other chemicals (NaOH, NaCl, menthol, dichloromethane, H2SO4, and hexane) were purchased from Beijing Chemical Works (Beijing, China).
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4

Modification of Balsa Wood Flour

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Balsa wood
flour was supplied by Zhuhai DE CHI Technology Co., Ltd. (Zhuhai,
China). All chemicals were used without further purification. Sodium
sulfite (Na2SO3, 98%), acryloyl chloride (AC)
(C3H3ClO, 98%), and tetraethylenepentamine (TEPA)
(C8H23N5, TP) were purchased from
Heowns Biochemical Technologies Co., Ltd. (Tianjin, China). Sodium
hydroxide (NaOH, 96%, AR), ethanol (CH3CH2OH,
99.7%, AR), hydrochloric acid (HCl, 37%, AR), dichloromethane (DCM)
(CH2Cl2, 99.5%, AR), and hydrogen peroxide (H2O2, AR) were obtained from Beijing Chemical Works
(Beijing, China). Lead dinitrate (Pb(NO3)2,
99%, AR) was purchased from Shanghai Macklin Biochemical Co., Ltd.
(Shanghai, China). Zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%, AR) was provided by Xilong Chemical
Co., Ltd. (Shantou, China), and copper nitrate trihydrate (Cu(NO3)2·3H2O, 99%, AR) was purchased
from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai,
China).
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5

Peptide Synthesis and Cell Viability Assay

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Fmoc-Phe-OH, Fmoc-Pro-OH, Fmoc-His(Trt)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Ala-OH, Rink amide-AM resin, 1-hydroxybenzotriazole, benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate, and N-methylmorpholine were obtained from GL Biochem (Shanghai, China). Dimethylformamide, methanol, dichloromethane, and n-hexane were obtained from Beijing Chemical Factory (Beijing, China). Pluronic® F-127 and DMSO were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dess–Martin periodinane was provided by Aladdin (Shanghai, China). DMEM medium was purchased from Thermo Fisher Scientific (Shanghai, China). Fetal bovine serum was purchased from Kang Yuan Biology (Tianjin, China). Trypsin and Hoechst 33258 nuclear dye were obtained from Solarbio (Beijing, China). Thiazolyl blue tetrazolium bromide (MTT) was purchased from Genview (Beijing, China). WST-1 kit and Lysotraker Green procured from Beyotime (Shanghai, China). Annexin V-FITC/PI apoptosis detection kit was purchased from BestBio (Shanghai, China).
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6

Lignin Modification and Characterization

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Industrial lignin was purchased from Changzhou Peaks Chemical Co., Ltd. (Changzhou, China). Its main composition was 93.35% lignin, 2.31% carbohydrates and 2.11% ash. N-methylimidazole, 1-chlorobutane and 1-bromoethane were purchased from Shanghai Jingchun Chemical Reagent Company; phosphoric acid, potassium acetate, toluene, dichloromethane, acetic anhydride, pyridine, chloroform and diethyl ether were purchased from Beijing Chemical Reagent Company, All reagents were used as supplied without further purification.
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7

Aroma Compound Quantification Protocol

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Pure standards of aroma compounds, namely, polyvinylpolypyrrolidone (PVPP) and 4-methyl-2-pentanol Methanol, were purchased from Sigma-Aldrich (St. Louis, MO, USA). β-d-glucolactone and n-octyl-β-d-glucopyranoside were purchased from Sangon Biotech (Shanghai, China). Rapidase® AR2000 commercial preparation with glycosidase side activities was purchased from DSM Oenology (Delft, The Netherlands). Methanol, dichloromethane, citric acid, and sodium phosphate dibasic dodecahydrate (analytical grade) were purchased from Beijing Chemical Works (Beijing, China). LC-MS grade acetonitrile, formic acid, and Methanol were purchased from Honeywell (Morristown, NJ, USA).
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8

Synthesis and Characterization of N-Heterocyclic Compounds

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The information of chemicals used in this work is as follows: bromine ethane (>99.0%), bromobutane (>99.0%), Aladdin Reagent Co. Ltd; N-methylimidazole (>99.0%), Shanghai SenHao Fine Chemical; ethyl acetate (≥99.5%), acetone (≥99.5%), dichloromethane (≥99.5%), acetic acid (≥99.5%), n-octane (≥99.0%), cyclohexane (AR), n-octene (≥98.0%), cyclohexene (≥99.0%), toluene (≥99.5%), Beijing Chemical Works; nitrogen (≥99.9%), Beijing Yanan Gas Plant; quinoline (AR), pyridine (≥99.5%), indole (≥99.0%), Tianjin Fuchen Chemical.
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9

Extraction and Purification of Amygdalin

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MS- and high performance liquid chromatography (HPLC)-grade acetonitrile, methanol, and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Analytical grade ethanol, methanol, dichloromethane, petroleum ether, sulfuric acid, aluminum chloride, and other reagents were purchased from Beijing Chemical Plant (Beijing, China). Ultrapure water was obtained from Watsons (Guangzhou, China). Column chromatography (CC) silica gel, thin layer silica gel G, and thin layer silica gel GF254 were purchased from Qingdao Marine Chemical Plant (Qingdao, China). AB-8 macroporous adsorption resin and Sephadex LH-20 gel were obtained from Solarbio (Beijing, China). SiliaSphere C18 was obtained from Silicycle (Quebec City, Canada). Dimethyl sulfoxide-d6 and methanol-d4 were purchased from Cambridge Isotopes Laboratories (Andover, MA, USA). Amygdalin standard compound (purity >98%) was obtained from Chengdu Pusi Biotechnology Co., Ltd. (Chengdu, China).
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10

Comparative Thermal Conductivity of Pitch-Based Carbon Fibers

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Two kinds of mesophase pitch-based carbon fibers with significant difference of axial thermal conductivity were used in this research. TC–HC–800 carbon fiber with yarn type of 2 K was provided by Shanxi Tiance New Materials Technology Co., Ltd, and the other pitch-based carbon fiber PCF-1 has onion-skin structure with yarn type of 6 K. The DGEBA epoxy resin E51 was produced by Nantong Xingchen Synthetic Material Co., Ltd, and the curing agent BC126 were supplied by Dasen (Tianjin) Material Science & Technology, Inc. Acetone and dichloromethane were purchased from Beijing Chemical Works.
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