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Smgm 2 bulletkit media

Manufactured by Lonza
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Sourced in Japan

The SmGM-2 BulletKit media is a cell culture medium formulated by Lonza specifically for the growth and maintenance of smooth muscle cells. It provides the necessary nutrients and growth factors to support the proliferation and survival of these cells in an in vitro setting. The composition of the media is optimized to support the specific requirements of smooth muscle cells.

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Lab products found in correlation

Hpasmc, by Thermo Fisher Scientific (2 mentions) Sc 1694, by Santa Cruz Biotechnology (1 mentions) C ebp, by Santa Cruz Biotechnology (1 mentions) Cc 2583, by Lonza (1 mentions) Ab4729, by Abcam (1 mentions) Model 3130, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SmGM-2 (60 citations) SmGM-2 BulletKit (19 citations) BulletKits (4 citations) SmGM-2 media (3 citations)

4 protocols using smgm 2 bulletkit media

1

Hypoxia Exposure of Pulmonary Artery Smooth Muscle Cells

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The PASMC line was purchased from Lonza (Swiss) and cultured in SmGM-2 BulletKit media (Lonza) containing 5% fetal bovine serum (FBS), growth factors, and antibiotics. Cells were incubated under 5% CO 2 at 37℃. PASMCs were exposed to hypoxia (1% O 2 , 5% CO 2 , and 1% FBS) for 0, 0.5, 1, 2, 4, 8, 12 and 24 h. The initial number of cells in each group was kept the same.
Ding J., Chu C., Mao Z., Yang J., Wang J., Hu L., Chen P., Cao Y., Li Y., Wan H., Wei D., Chen J., Chen F, & Yu Y. (2022). Metabolomics-based mechanism exploration of pulmonary arterial hypertension pathogenesis: novel lessons from explanted human lungs. Hypertension research : official journal of the Japanese Society of Hypertension, 45(6).
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2

Culturing Human Pulmonary Artery Smooth Muscle Cells

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Human pulmonary artery smooth muscle cells (hPASMCs), purchased from Cascade Biologics Inc (Portland, Oregon, USA), were cultured with SmGM‐2 BulletKit media (Lonza Japan, Tokyo, Japan) supplemented with 5% foetal bovine serum (FBS; Hyclone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin in a humidified incubator with 5% CO2 at 37˚C. For hypoxia environment, hPASMCs were exposed to 1% O2 for indicated times.
Song R., Lei S., Yang S, & Wu S. (2021). LncRNA PAXIP1‐AS1 fosters the pathogenesis of pulmonary arterial hypertension via ETS1/WIPF1/RhoA axis. Journal of Cellular and Molecular Medicine, 25(15), 7321-7334.
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3

Mapping Transcription Factor Binding in Vascular Cells

Cited 1 time
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Data from ENCODE26 (link) were explored as described in eMethods. ChIP-seq experiments were performed on confluent HCASMC (Cell Applications 350-05a & Lonza CC-2583; cultured in SmGM-2 BulletKit media; Lonza) as described.27 (link) TCF21 (Abcam ab49475), Jun (Santa Cruz Biotechnology sc-1694), JunD (Santa Cruz Biotechnology sc-74), and CEBP (Santa Cruz Biotechnology sc-150) transcription factor binding was interrogated and H3K27ac data were acquired using the same ChIP protocol with an anti-H3K27ac antibody (Abcam; ab4729). Reads were aligned to the human genome (GRCh37p13) using STAR.28 (link)
Saleheen D., Zhao W., Young R., Nelson C.P., Ho W., Ferguson J.F., Rasheed A., Ou K., Nurnberg S.T., Bauer R.C., Goel A., Do R., Stewart A.F., Hartiala J., Zhang W., Thorleifsson G., Strawbridge R.J., Sinisalo J., Kanoni S., Sedaghat S., Marouli E., Kristiansson K., Zhao J.H., Scott R., Gauguier D., Shah S.H., Smith A.V., van Zuydam N., Cox A.J., Willenborg C., Kessler T., Zeng L., Province M.A., Ganna A., Lind L., Pedersen N.L., White C.C., Joensuu A., Kleber M.E., Hall A.S., März W., Salomaa V., O’Donnell C., Ingelsson E., Feitosa M.F., Erdmann J., Bowden D.W., Palmer C.N., Gudnason V., De Faire U., Zalloua P., Wareham N., Thompson J.R., Kuulasmaa K., Dedoussis G., Perola M., Dehghan A., Chambers J.C., Kooner J., Allayee H., Deloukas P., McPherson R., Stefansson K., Schunkert H., Kathiresan S., Farrall M., Frossard P.M., Rader D.J., Samani N.J, & Reilly M.P. (2017). Loss of Cardio-Protective Effects at the ADAMTS7 Locus Due to Gene-Smoking Interactions. Circulation, 135(24), 2336-2353.
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4

Culturing and Hypoxic Stimulation of hPASMCs

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Human pulmonary artery smooth muscle cells (hPASMCs; Cascade Biologics Inc., Portland, OR) were cultured in SmGM-2 BulletKit media (Lonza, Basel, Switzerland) containing 5% (volume/volume) heat-inactivated fetal bovine serum (FBS; Gibco, Carlsbad, CA), 0.5 ng/ml human recombinant epidermal growth factor, 2 ng/ml human recombinant fibroblast growth factor, 5 μg/ml insulin, and 50 μg/ml gentamicin. HEK293 cells were maintained in DMEM supplemented with 10% heat-inactivated FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml) at 37°C in a humidified atmosphere containing 5% CO2. Cells at passages 6–8 were used for experiments. For induction of hypoxia, cells were transferred in a special hypoxia incubator (Thermo Scientific, model 3130, Rockford, IL) with 3% O2, 5% CO2, and balanced nitrogen. The O2 concentration inside the chamber was detected continuously by using an oxygen monitor (Hudson Ventronics Division, CA) to ensure that the O2 concentration was 3%.
Lu Z., Li S., Zhao S, & Fa X. (2016). Upregulated miR-17 Regulates Hypoxia-Mediated Human Pulmonary Artery Smooth Muscle Cell Proliferation and Apoptosis by Targeting Mitofusin 2. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 3301-3308.
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