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2 protocols using vmrc rcw

1

Cell lines for cancer research

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The human mesothelioma cell lines ACC-MESO-1 and ACC-MESO-4 were purchased from the RIKEN Cell Bank (Ibaraki, Japan), JMN was a kind gift from Dr. Brenda Gerwin and others (NCI-H226, MSTO-211H, NCI-H28 and NCI-H2452) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). The lung cancer cell lines (A549, PC9, Lu24, and WA-hT), and the HuH-7 (hepatocellular carcinoma), MKN1 (gastric cancer), OVK18 (ovarian cancer), VMRC-RCW (renal cell carcinoma) cell lines were purchased from the RIKEN Cell Bank. The MCF7 (breast cancer), KP3 (pancreatic cancer), and HCT 116 (colon cancer) cell lines were purchased from ATCC. All cells were cultured and maintained in RPMI-1640 medium (GIBCO, Grand Island, NY) supplemented with 10 % heat-inactivated fetal calf serum.
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2

Culturing Renal Cell Carcinoma and HEK293T

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Renal cell carcinoma (OS-RC-2, TUHR14TKB, and VMRC-RCW) and HEK293T cell lines were obtained from the Riken Cell Bank (Tsukuba, Japan). Renal cell carcinoma and HEK293T cells were cultured in RPMI and DMEM, respectively, containing 10% FBS and penicillin/streptomycin.
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