Phospholipon 90G (P90G) was purchased from Lipoid (Steinhausen, Switzerland). CsA (purity > 99%), poly-(vinyl alcohol) (PVA) (Mw = 9-10 kDa, 80% hydrolysed), sodium dodecyl sulphate (SDS), 3-aminophtalhydrazinde monosodium salt (luminol, purity > 98%), oxazolone (purity > 99%) and ammonium molybdate (purity > 99%) were purchased from Sigma-Aldrich (St. Louis, USA). Polysorbate 80 (Tween ® 80) and L-( +)-ascorbic acid (purity > 99%) were purchased from Scharlab (Sentmenat, Spain). HPLC-quality methanol (MeOH), ethanol (EtOH) and acetonitrile (ACN) were obtained from Sigma-Aldrich (St. Louis, USA). Acetone (purity 99.9%) was purchased from ACROS Organics (Geel, Belgium). Ultrapure water (UPW) was obtained by a Milli-Q purification system with resistance > 18 MΩ cm and TOC < 10 ppb. Poly-(vinyl pyrrolidone) (PVP) (Mw = 40 kDa) was acquired from Tokyo Chemical Industry (Tokyo, Japan). L-( +)-ascorbic acid (purity > 99%) was purchased from Scharlab (Sentmenat, Spain). TNF-α, IL-4, IL-1β and IL-6 ELISA kits were acquired from ThermoFisher Scientific (Waltham, USA).
L ascorbic acid
Manufactured by Scharlab
Sourced in Spain
L (+) ascorbic acid is a chemical compound that serves as a common form of vitamin C. It is a white, crystalline solid that is highly soluble in water. As a laboratory reagent, L (+) ascorbic acid is used as an antioxidant, reducing agent, and pH adjuster in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Dl dithiothreitol solution, by Scharlab (2 mentions)
Oxalic acid, by Scharlab (2 mentions)
Md 1510, by Jasco (1 mentions)
Milli q model pacific tii 12, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (1 mentions)
Formic acid, by Honeywell (1 mentions)
Calcium chloride cacl2, by Scharlab (1 mentions)
Ammonium molybdate, by Merck Group (1 mentions)
Acetone, by Thermo Fisher Scientific (1 mentions)
Poly vinyl pyrrolidone pvp, by Tokyo Chemical Industry (1 mentions)
Acetonitrile acn, by Merck Group (1 mentions)
Oxazolone, by Merck Group (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Il 6 elisa kit, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
7 protocols using l ascorbic acid
1
Optimized Liposomal Formulation for Topical Delivery
2
Vitamin C Determination in Fruit Purees
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Total vitamin C content (VC) was determined by the reduction of dehydroascorbic acid to ascorbic acid (AA) using high-performance liquid chromatography (HPLC) (Jasco, Italy). The reduction was carried out by mixing 0.5 g of FOP or 0.075 g of each of the 12 freeze-dried puree samples with 2 mL of a 20 g/L DL-dithiothreitol solution (Scharlab, Spain) for 2 h at room temperature and under darkness [35 (link),36 (link)]. The extraction of the mixture was carried out according to Xu et al. [37 (link)]. The HPLC conditions were: Kromaphase100-C18, 5 mm (4.6 × 250 mm) column (Scharlab SL); mobile phase 0.1% oxalic acid, volume injected 10 μL, flow rate 1 mL/min, detection at 243 nm (detector UV-visible MD-1510, Jasco, Cremella, Italy) at 25 °C. A standard solution of L (+) ascorbic acid (Scharlab SL, Sentmenat, Spain) in the range of 5–200 ppm was prepared. The VC content was calculated as mg AA/100g db sample and the percentage (%) of this bioactive compound preserved in the FDP in reference to the FOP was calculated (Equation (7)). Three replicates were performed per sample.
3
Antioxidant Compound Characterization Protocol
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(±)-6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (TROLOX) was purchased from Sigma Aldrich (St. Louis, MO, USA); 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) from Alfa Aesar (Thermo Fisher Scientific, Waltham, MA, USA); potassium persulfate from VWR Chemicals BDH (Radnor, PA, USA); and L -(+)-ascorbic acid from Scharlab S.L. (Barcelona, Spain).
Ethanol, mEthanol, and acetonitrile, all LC-MS grade, were purchased from Sigma Aldrich. Formic acid (LC-MS grade) was purchased from Honeywell Research Chemicals (Muskegon, MI, USA).
Milli-Q water was obtained using Ultrapure Water Systems (GenPure UV-TOC/UF × CAD plus) connected to the Milli-Q Water Purification System (<0.055 μS/cm, Milli-Q Model Pacific TII 12, Thermo Scientific, Waltham, MA, USA, NOW).
Ethanol, mEthanol, and acetonitrile, all LC-MS grade, were purchased from Sigma Aldrich. Formic acid (LC-MS grade) was purchased from Honeywell Research Chemicals (Muskegon, MI, USA).
Milli-Q water was obtained using Ultrapure Water Systems (GenPure UV-TOC/UF × CAD plus) connected to the Milli-Q Water Purification System (<0.055 μS/cm, Milli-Q Model Pacific TII 12, Thermo Scientific, Waltham, MA, USA, NOW).
4
Calcium-Alginate Microparticles with Iron Supplement
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The reagents used in the preparation of the samples, together with the buffer, were calcium chloride (CaCl2) (Scharlab SL, Barcelona, Spain), sodium alginate (Panreac Química SLU, Castellar del Valles, Barcelona, Spain) with a purity of 90%, iron-protein- succinylate (Ferplex, Italfarmaco SA, Madrid, Spain), with a purity of 40 mg/15 mL, L(+)-Ascorbic acid (Scharlab SL, Barcelona, Spain) and hydrochloric acid (1N) (Panreac Química SLU, Castellar del Valles, Barcelona, Spain).
5
Lipid Membrane Composition and Peptide Interactions
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1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), L-α-lysophosphatidylcholine (Egg Lecithin, PC), L-α-phosphatidylethanolamine (Liber Bovine, PE), 1′,3′-bis [1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (Cardiolipin 18:1, CL), phosphatidyl serine (PS), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA), while 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG) and N-Acyl-D-sphingosine-1-phosphocholine (chicken egg yolk, SM) were provided by Larodan AB (Solna, Sweden) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Tris-HCl, Tris base, Sephadex G-100, 5-carboxyfluorescein (≥95%, HPLC), nitromethane (Reagent Plus, ≥99%), Triton™ X-100 and acrylamide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium Dodecyl Sulfate (SDS, 20% Solution) and Luria Broth (LB) were purchased from Thermo Fisher (Bremen, Germany). Ammonium heptamolybdate tetrahydrate ((NH4)Mo7O24·4H2O), di-sodium hydrogen phosphate anhydrous (Na2HPO4), L(+)-Ascorbic acid and all the other reagents were obtained from Scharlau (Barcelona, Spain). The derivative 12-mer peptide 1018-K6 was purchased from SynPeptide Co., Ltd. (Shanghai, China).
6
Vitamin C Quantification in Grapefruit
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Vitamin C (VC) was determined by high-performance liquid chromatography (HPLC) (Jasco, Italy). For the analysis, 0.075 g of powder was used. The procedure employed was the reduction of dehydroascorbic acid to ascorbic acid, using DLdithiothreitol (Scharlab S.L, Spain) as the reductant reagent, and extraction with oxalic acid (Scharlab S.L, Spain) (Igual et al., 2014; (link)Xu et al., 2008) (link). The HPLC conditions were: KROMAPHASE100-C18, 5mm (4.6x250mm) column (Scharlab S.L, Spain); mobile phase 0.1 % oxalic acid, volume injection 20 L, flow rate 1mL/min, detection at 243 nm (detector UV-visible MD-1510) and at 25 ºC. A standard solution of L(+) ascorbic acid (Scharlab S.L, Spain) solution was prepared. The vitamin C content was calculated as mg of ascorbic acid per 100 g of the grapefruit's own solutes (mg AA/100gGS, Agudelo et al., 2017) (link).
7
Determination of Total Vitamin C Content
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Total VCC was determined reducing dehydroascorbic acid to ascorbic acid [16] , by highperformance liquid chromatography (HPLC) (Jasco, Cremella, Italy). In brief, 0.5 g of powder were mixed with 2 ml of a 20 g/l DL-dithiothreitol solution (Scharlab S.L., Barcelona, Spain) for 2 h at room temperature and in a dark condition. Afterwards, VCC was extracted [17] . For that, 1 g of this mixture was extracted with 9 ml 0.1% oxalic acid (Scharlab S.L, Barcelona, Spain) under stirring for 3 min and filtered through a 0.45 m membrane filter. Finally, the VCC was determined by HPLC with the following conditions: Kromaphase 100-C18 column (4.6 x 250 mm, 5 mm) (Scharlab S.L, Barcelona, Spain); mobile phase 0.1% oxalic acid, 20 ml volume injection, 1ml/min flow rate. Detection was made at 25ºC and at 243 nm using a detector UV-visible MD-1510. A standard solution of L (+) ascorbic acid (Scharlab S.L., Barcelona, Spain) in the range of 10-530 ppm was prepared.
The VCC was calculated as g of ascorbic acid (AA) per kg of OS (g AA/ kg OS).
The VCC was calculated as g of ascorbic acid (AA) per kg of OS (g AA/ kg OS).
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