Anti apoe

Manufactured by Merck Group

Sourced in France

Anti-apoE is a laboratory reagent that binds to and detects the apolipoprotein E (apoE) protein. ApoE is a key component involved in the transport and metabolism of cholesterol and other lipids in the body. The primary function of Anti-apoE is to facilitate the identification and quantification of apoE in biological samples through various analytical techniques.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant apoe isoforms, by BioCat (2 mentions) Apoe3, by Romer Labs (2 mentions) Apoe 75 85, by Cell Biolabs (2 mentions) Apoe 139 152, by Cell Biolabs (2 mentions) Apoe 210 232, by Cell Biolabs (2 mentions) Mda ldl, by Cell Biolabs (2 mentions) Copper oxidized ldl oxldl, by Thermo Fisher Scientific (2 mentions) Anti c5b 9 clone ae11, by Agilent Technologies (2 mentions) Anti aβ clone32a1, by AbFrontier (2 mentions) Anti map2, by Merck Group (2 mentions) Anti lc3b, by Merck Group (1 mentions) Bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Anti na k atpase, by Abcam (1 mentions) Anti gpx4, by Abcam (1 mentions) Anti ferritin, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Protease inhibitor, by Roche (1 mentions) Ab18180, by Abcam (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Sc 20691, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Goat anti-ApoE (4 citations) Goat anti-apoE Ab (3 citations) Anti-APOE antibody (2 citations)

11 protocols using anti apoe

Characterization of Complement-ApoE Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Complement components C2, C3, C3b, C4, C4b, C1q, C1s, Factor H, Factor
I, and C4BP as well as all primary antibodies (anti C1q, A200/3b; anti C2,
A212/18b; anti C3, A213/5a; anti C4, A201/3a; anti factor H, A237/4) were
purchased from Complement Technology. Recombinant ApoE isoforms from BioCat;
plasma-purified ApoE3 from Biopure. ApoE peptides were generated by Peptide 2.0:
ApoE 30-40 LGRFWDYLRWV; ApoE 75-85 YKSELEEQLTPV; ApoE 139-152 SHLRKLRKRLLRDA;
ApoE 210-232 WGERLRARMEEMGSRTRDRLDEV. LDL and malondialdehyde-modified LDL
(MDA-LDL) were from Cell Biolabs, copper oxidized LDL (oxLDL) from Thermo Fisher
(L34357), ApoA from Athens Research&Technology, vitronectin (Vnt) from
Corning. Aβ and Aβ fibrils from GenSript. Recombinant EfB was
expressed as described65 . Additional
antisera used were: anti-C5b-9 (clone AE11-MO777-Dako), anti C1s
(11951-05011-AssaybioTch), anti ApoE ((178479-Calbiochem), anti ApoE (Merck,
178479), anti LDLR (HB04JL2204-B-SinoBiological), anti Aβ (clone
32A1-Abfrontier), anti goat IgG (13C0836-Sigma Aldrich) and anti rabbit IgG
(13C0529-Sima Aldrich), IgM (120228-Jackson ImmunoResearch Laboratories).

Yin C., Ackermann S., Ma Z., Mohanta S.K., Zhang C., Li Y., Nietzsche S., Westermann M., Peng L., Hu D., Bontha S.V., Srikakulapu P., Beer M., Megens R.T., Steffens S., Hildner M., Halder L.D., Eckstein H.H., Pelisek J., Herms J., Roeber S., Arzberger T., Borodovsky A., Habenicht L., Binder C.J., Weber C., Zipfel P.F., Skerka C, & Habenicht A.J. (2019). ApoE attenuates unresolvable inflammation by complex formation with activated C1q. Nature medicine, 25(3), 496-506.

+ Open protocol

+ Expand

Characterization of Complement-ApoE Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Protein Expression and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted from cell pellets using RIPA buffer (50 mM Tris, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, containing protease inhibitors (Roche)). 15 μg aliquots of protein were separated by SDS-PAGE on 4–20% Bis-Tris protein gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF) membrane. Primary antibodies used were anti-Ferritin (cat#ab75973, Abcam), anti-ApoE (cat#AB947, Merck), anti-LC3B (cat#L7543, Sigma Aldrich), anti-GPX4 (cat#ab125066, Abcam), anti-Na/K-ATPase (cat#ab7671, Abcam), anti-AKT/pAKT (cat#4691S/4060S, Cell Signaling), anti-TfR1 (cat#TFR12-M, Alpha Diagnostic) and anti-β-actin (cat#A5441, Sigma). Membranes were probed with horseradish peroxidase-conjugated secondary antibodies and signal was detected using a LAS-4000 luminescence Imaging analyzer (GE Healthcare Life science). Densitometry analyses were carried out using Image J software and quantitation was normalized to β-actin levels.

Belaidi A.A., Masaldan S., Southon A., Kalinowski P., Acevedo K., Appukuttan A.T., Portbury S., Lei P., Agarwal P., Leurgans S.E., Schneider J., Conrad M., Bush A.I, & Ayton S. (2022). Apolipoprotein E potently inhibits ferroptosis by blocking ferritinophagy. Molecular psychiatry.

+ Open protocol

+ Expand

Western Blot Analysis of APOE, ABCA1, APP, DLK1 and KLF4

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen cortices and hippocampi were homogenized in TBS homogenization buffer (250 mM sucrose, 20 mM Tris base, 1 mM EDTA, and 1 mM EGTA, 1 ml per 100 mg of tissue) and protease inhibitors cocktail (Roche). For WB, proteins extracted with TBS or RIPA buffer were resolved on SDS-PAGE and transferred onto nitrocellulose membranes. For APOE protein detection, TBS soluble brain extract was used and RIPA extracted proteins were used for ABCA1, APP, DLK1 and KLF4 detection. Thirty microgram of proteins were resolved on 10 or 12% SDS-PAGE gels and transferred onto nitrocellulose membranes. Used were the following primary antibodies: Anti-ABCA1 (Ab18180, Abcam), anti-ApoE (178479, Millipore), anti-APP (6E10 antibody, 9320–02, Covance), anti-DLK1 (sc-25437, Santa Cruz), anti-GAPDH (sc-25778, Santa Cruz), anti-KLF4 (sc-20691, Santa Cruz).

Nam K.N., Mounier A., Wolfe C.M., Fitz N.F., Carter A.Y., Castranio E.L., Kamboh H.I., Reeves V.L., Wang J., Han X., Schug J., Lefterov I, & Koldamova R. (2017). Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer’s model mice. Scientific Reports, 7, 4307.

+ Open protocol

+ Expand

ELISA for ApoE and Aβ42 in brain extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

ApoE and Aβ42 were measured by enzyme-linked immunosorbent assay (ELISA) in the PBS, TBS-X, and FA extracts. The apoE ELISA was performed as described in Tai et al. (2014) (link) using anti-apoE (1:2000, Millipore) and biotinylated anti-apoE (1:5000, Meridian) for capture and detection antibodies, respectively. Aβ42 was measured by a commercially available ELISA kit following the manufacturer instructions (Life Technologies).

Marottoli F.M., Katsumata Y., Koster K.P., Thomas R., Fardo D.W, & Tai L.M. (2017). Peripheral Inflammation, Apolipoprotein E4, and Amyloid-β Interact to Induce Cognitive and Cerebrovascular Dysfunction. ASN NEURO, 9(4), 1759091417719201.

+ Open protocol

+ Expand

Quantifying ApoE, Aβ40, and Aβ42 in E4FAD+ Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

ApoE and Aβ40 and Aβ42 were measured by ELISA in the SDS and FA extracts (different plates for each extract) of E4FAD+ mice. The apoE ELISA was performed using anti-apoE (1:2,000, Millipore) and biotinylated anti-apoE (1:5000, Meridian) for capture and detection antibodies, respectively, as described in [47 (link)]. Aβ40, Aβ42 (Life Technologies) and EGF (R&D systems) were measured by a commercially available ELISA kit following the manufacturer instructions. ApoE, Aβ40, Aβ42 and EGF levels were normalized to total protein levels in each extract.

Zaldua S., Damen F.C., Pisharody R., Thomas R., Fan K.D., Ekkurthi G.K., Scheinman S.B., Alahmadi S., Marottoli F.M., Alford S., Cai K, & Tai L.M. (2020). Epidermal growth factor treatment of female mice that express APOE4 at an age of advanced pathology mitigates behavioral and cerebrovascular dysfunction. Heliyon, 6(5), e03919.

+ Open protocol

+ Expand

Hippocampal Protein Levels in Neurodegeneration

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Apolipoprotein E (ApoE), Aβ42, and Angiotensin II levels were measured in hippocampal homogenates by ELISA. The apoE ELISA was performed using anti-apoE (1:2000, Millipore) and biotinylated anti-apoE (1:5000, Meridian) for capture and detection antibodies as described in Thomas et al. (2016 (link), 2017) (link), Marottoli et al. (2017) (link). Aβ42 (Life Technologies) and Angiotensin II (Cloud-Clone Corp.) were measured following the manufacturer instructions. ApoE, Aβ42, and Angiotensin II levels were normalized to total protein levels in each of the distinct fractions. In addition, we calculated total Aβ42 and total apoE levels using the following equation:

Scheinman S.B., Zaldua S., Dada A., Krochmaliuk K., Dye K., Marottoli F.M., Thatcher G.R, & Tai L.M. (2021). Systemic Candesartan Treatment Modulates Behavior, Synaptic Protein Levels, and Neuroinflammation in Female Mice That Express Human APOE4. Frontiers in Neuroscience, 15, 628403.

+ Open protocol

+ Expand

Quantification of ApoE and Inflammation Markers

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

ApoE was measured by ELISA in brain endothelial cell–enriched lysates (1:8 dilution), cortical lysates (1:160 dilution), and plasma (1:20 000 dilution) as described in previous studies^{11 (link),28 (link)} using anti-apoE (1:2000; Millipore) and biotinylated anti-apoE (1:5000; Meridian) for capture and detection antibodies, respectively. Cortical lysates were analyzed for fibrinogen (1:3 dilution), IgG (1:3 dilution), and albumin (1:100 dilution) with commercial ELISA kits (Immunology Consultants) with 2 minor modifications to the manufacturer’s protocol; sample incubation time was increased to 2 hours at 37 °C, and secondary antibody was increased to 1 hour at 37 °C, and standards were buffer matched. Cortical samples were normalized to protein concentration, and isolated brain endothelial cell samples were normalized to actin quantified by the Western blot. Plasma levels (1:10 dilution) of cardiovascular dysfunction were measured using MILLIPLEX MAP CVD magnetic bead panel according to the manufacturer’s protocol.

Marottoli F.M., Zhang H., Flores-Barrera E., Artur de la Villarmois E., Damen F.C., Miguelez Fernández A.M., Blesson H.V., Chaudhary R., Nguyen A.L., Nwokeji A.E., Talati R., John A.S., Madadakere K., Lutz S.E., Cai K., Tseng K.Y, & Tai L.M. (2023). Endothelial Cell APOE3 Regulates Neurovascular, Neuronal, and Behavioral Function. Arteriosclerosis, Thrombosis, and Vascular Biology, 43(10), 1952-1966.

+ Open protocol

+ Expand

Immunostaining of Primary Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary neurons on coverslips were washed 10min in dPBS, then permeabilized in dPBS + 0.1% Triton-X for 5min at RT. The neurons were then blocked in 10% normal donkey serum + 0.5% Triton in PBS for 1hr, and further blocked with mouse-on-mouse (MOM) blocking buffer (Vector Labs, MKB-2213-1) at 1 drop Mouse IgG block buffer per 4ml PBS. Primary antibodies were diluted in MOM Antibody dilution buffer (80ul protein concentrate to 1ml PBS), and cells were incubated in primary antibody solution overnight at 4°C (anti-ApoE 1:5000, Millipore 178479; anti-MHC-I (OX18) 1:50, Santa Cruz Biotechnologies sc-53074; anti-MAP2 1:500, Millipore ab5622; anti-p-Tau (PHF1) 1:300, from Peter Davis). The next day, cells were washed 3x (15min, 10min, 5min) in PBS with 0.1% Triton-X. Secondary antibodies were diluted in MOM Antibody Dilution Buffer (1:1500), and incubated with the cells for 1hr at RT. Cells were again washed 3x (15min, 10min, 5min) in PBS with 0.1% Triton-X. Coverslips were then mounted to microscope slides (Vectashield, Prolong Gold).

Zalocusky K.A., Najm R., Taubes A.L., Hao Y., Yoon S.Y., Koutsodendris N., Nelson M.R., Rao A., Bennett D.A., Bant J., Amornkul D.E., Xu Q., An A., Cisne-Thomson O, & Huang Y. (2021). Neuronal ApoE Upregulates MHC-I Expression to Drive Selective Neurodegeneration in Alzheimer’s Disease. Nature neuroscience, 24(6), 786-798.

+ Open protocol

+ Expand

Western Blotting Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described^{44 (link)}. List of antibodies used: Actin (Sigma, A5316); Anti-phospho-JNK1/2 (Cell signalling, Ipswich, MA, USA), Anti-phospho-ERK1/2 (Cell signalling, Ipswich, MA, USA), Anti- phosphop-38 (Cell signalling, Ipswich, MA, USA) and total p38 MAPK (Cell signaling, Ipswich, MA, USA), JNK1/2 (Cell signaling, Ipswich, MA, USA) and ERK1/2. Anti-SAA (Santa Cruz Biotechnology, CA), Anti-Apo E (Millipore), Albumin (Santa Cruz Biotechnology, CA), and Anti- LBP (Santa Cruz, CA).

Adhikari A., Martel C., Marette A, & Olivier M. (2017). Hepatocyte SHP-1 is a Critical Modulator of Inflammation During Endotoxemia. Scientific Reports, 7, 2218.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!