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Recombinant rat jag1 human fc igg chimeric protein

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Recombinant rat JAG1/human Fc IgG chimeric protein is a fusion protein composed of the extracellular domain of the rat Jagged 1 protein and the Fc region of human IgG. It is produced in a mammalian cell expression system.

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Recombinant proteins (13 citations) Jag1-Fc (7 citations) Human IgG Fc chimeric proteins (2 citations) Recombinant human Jag1 (2 citations)

2 protocols using recombinant rat jag1 human fc igg chimeric protein

1

Activating Notch Signaling in Stromal Cells

Cited 1 time
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Stromal cells were harvested from femurs and tibias of Thbs2-null and control mice and cultured as previously described.26 (link) At 80% confluence, cells were trypsinized and plated on 12-well tissue culture plates at a density of 1 × 105 cells per well. Notch signaling was activated by precoating tissue culture plates with 10 μg/mL of antibody against the Fc portion of human IgG (Jackson ImmonoResearch, West Grove, PA) for 1 hour and then incubating with the indicated concentration of recombinant rat JAG1/human Fc IgG chimeric protein (R&D Systems) for 2 hours as previously described.27 (link) For Notch reporter activity experiments, Fc-JAG1 or TNF-related apoptosis-inducing ligand (control) conditioned media28 (link) were used to coat plates instead of the recombinant Fc-JAG1 protein.
Tsai E.A., Gilbert M.A., Grochowski C.M., Underkoffler L.A., Meng H., Zhang X., Wang M.M., Shitaye H., Hankenson K.D., Piccoli D., Lin H., Kamath B.M., Devoto M., Spinner N.B, & Loomes K.M. (2016). THBS2 Is a Candidate Modifier of Liver Disease Severity in Alagille Syndrome. Cellular and Molecular Gastroenterology and Hepatology, 2(5), 663-675.e2.
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2

Notch Signaling Activation in Stromal Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Stromal cells were harvested from femurs and tibias of Thbs2-null and control mice and cultured as previously described.26 (link) At 80% confluence, cells were trypsinized and plated on 12-well tissue culture plates at a density of 1 × 105 cells per well. Notch signaling was activated by precoating tissue culture plates with 10 μg/mL of antibody against the Fc portion of human IgG (Jackson ImmonoResearch, West Grove, PA) for 1 hour and then incubating with the indicated concentration of recombinant rat JAG1/human Fc IgG chimeric protein (R&D Systems) for 2 hours as previously described.27 (link) For Notch reporter activity experiments, Fc-JAG1 or TNF-related apoptosis-inducing ligand (control) conditioned media28 (link) were used to coat plates instead of the recombinant Fc-JAG1 protein.
Tsai E.A., Gilbert M.A., Grochowski C.M., Underkoffler L.A., Meng H., Zhang X., Wang M.M., Shitaye H., Hankenson K.D., Piccoli D., Lin H., Kamath B.M., Devoto M., Spinner N.B, & Loomes K.M. (2016). THBS2 Is a Candidate Modifier of Liver Disease Severity in Alagille Syndrome. Cellular and Molecular Gastroenterology and Hepatology, 2(5), 663-675.e2.
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