For quantification of GFP and bioluminescence, overnight bacterial cultures of different strains with plasmid pGLhla were diluted in an appropriate medium containing 10 µg/mL chloramphenicol. Samples (100 µL) were transferred into a microtiter plate from culture tubes, and fluorescence was measured by using a DTX 800/880 multimode plate reader (Beckman). Bacteria with pGL plasmid were included as a control to allow correction for background fluorescence. For growth curve analysis, 100 µL of 106S. aureus strains were loaded to 96-well microtiter plates in triplicate and incubated at 37°C. The optical density at 620 nm (OD620), the fluorescence (GFP, Ex/Em: 485/535), and the bioluminescence were measured every 30 min in the DTX 800/880 multimode plate reader (Beckman).
Dtx 800 880 multimode plate reader
Manufactured by Beckman Coulter
The DTX 800/880 multimode plate reader is a versatile laboratory instrument designed for performing a wide range of assays in a 96-well or 384-well microplate format. The device is capable of detecting and quantifying various types of signals, including absorbance, fluorescence, and luminescence, making it suitable for numerous applications in life science research and drug discovery.
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Lab products found in correlation
2 protocols using dtx 800 880 multimode plate reader
1
Quantifying Bacterial GFP and Bioluminescence
2
Quantifying Bacterial GFP and Bioluminescence
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For quantification of GFP fluorescence and bioluminescence, overnight bacterial cultures were diluted in appropriate media containing 10 μg/ml chloramphenicol. Samples (100 μL) were transferred into microtiter plate from culture tubes, and fluorescence was measured by using DTX 800/880 multimode plate reader (Beckman). Bacteria with pGL plasmid were included as control to allow correction for background fluorescence.
For bacteria growth curve monitoring, samples (100 μL) with 106S. aureus were separated into aliquots in triplicate into clear-bottom 96-well microtiter plates and incubated at 37°C. The optical density at 620 nm (OD620), the fluorescence (GFP), and the bioluminescence were measured every 30min in DTX 800/880 multimode plate reader (Beckman).
For bacteria growth curve monitoring, samples (100 μL) with 106S. aureus were separated into aliquots in triplicate into clear-bottom 96-well microtiter plates and incubated at 37°C. The optical density at 620 nm (OD620), the fluorescence (GFP), and the bioluminescence were measured every 30min in DTX 800/880 multimode plate reader (Beckman).
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