Goat anti rabbit cy5

Manufactured by Abcam

Sourced in United States

Goat anti-rabbit Cy5 is a secondary antibody that binds to rabbit primary antibodies and is conjugated with the Cyanine 5 (Cy5) fluorescent dye. Cy5 has excitation and emission wavelengths of approximately 649 nm and 670 nm, respectively, enabling detection in the red/far-red region of the visible spectrum.

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Lab products found in correlation

Orb10314, by Biorbyt (1 mentions) Goat anti mouse cy3, by Abcam (1 mentions) Normal goat serum (ngs), by Thermo Fisher Scientific (1 mentions) Dp71 fluorescence microscope, by Olympus (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Donkey serum, by Abcam (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Rabbit anti-

3 protocols using goat anti rabbit cy5

Quantifying Blood Vessel Density in Hypertrophic Scars

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Blood vessel density was assessed using co‐immunostaining for α‐smooth muscle actin (α‐SMA) and cluster of differentiation factor 31 (CD31) as previously described.²³ Co‐staining was used due to the fact that immature blood vessels such as those in remodeling hypertrophic scars may not have full pericyte coverage that could be identified with α‐SMA alone.²⁵ Biopsies taken at baseline representing normal skin (n = 4 Dc, n = 4 Yk) and at day 98 representing HTS (n = 4 Dc, n = 4 Yk) were sectioned and stained using primary antibodies for α‐SMA (Abcam, ab7817, 1:250, mouse) and CD31 (Biorbyt, orb10314, 1:500, rabbit) and secondary antibodies (goat anti‐mouse‐CY3 and goat anti‐rabbit‐CY5, Abcam). Two photos of the papillary dermis were taken at 10× magnification for each biopsy. Blood vessel density was calculated by counting the number of vessels and dividing by the area. Secondary antibody controls were stained in parallel and did not show nonspecific staining.

Funkhouser C.H., Kirkpatrick L.D., Smith R.D., Moffatt L.T., Shupp J.W, & Carney B.C. (2021). In‐depth examination of hyperproliferative healing in two breeds of Sus scrofa domesticus commonly used for research. Animal Models and Experimental Medicine, 4(4), 406-417.

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Immunofluorescence Analysis of NF-κB and Caspase-3 in MCF-7 Cells

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This method was used to determine the expression of NF-κB p65, caspase-3 in MCF-7 cells. The cells were seeded onto glass cover-slips placed in a 24-well plate (5×10⁵ cells/well). The groups and treatments were as described above. The cells were treated with various concentrations of the pigment compound VI. Following treatment, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature. The cells were blocked with 10% normal goat serum (Gibco) prior to incubation with rabbit anti-NF-κB p65, caspase-3 (Santa Cruz Biotechnology, Inc.) or negative control rabbit IgG (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd.) overnight at 4°C. After rinsing with PBS, the cells were incubated with goat anti-rabbit Cy5 (Abcam, Cambridge, MA, USA) and 1 µg/ml of Hoechst 33342 (Sigma) for 1 h at room temperature. Immunolabelling was visualized and imaged using an Olympus DP71 fluorescence microscope (Olympus, Center Valley, PA, USA).

Zheng L., Cai Y., Zhou L., Huang P., Ren X., Zuo A., Meng X., Xu M, & Liao X. (2016). Benzoquinone from Fusarium pigment inhibits the proliferation of estrogen receptor-positive MCF-7 cells through the NF-κB pathway via estrogen receptor signaling. International Journal of Molecular Medicine, 39(1), 39-46.

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Immunohistochemical Staining of Bone Markers

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Tissue sections were dried for 30 minutes at room temperature and then rehydrated by rinsing in PBS for 15 minutes. Aldehydes were quenched with 0.1% Glycine for 5 mins. Sections were then permeabilized for 30 minutes in 0.1% Triton X-100 (Sigma) at room temperature. Afterwards, sections were washed twice for 10 minutes in PBS at room temperature. Non-specific staining was blocked with a 1% BSA and 5% donkey or goat serum solution (Invitrogen) in PBS for one hour. The blocking solution was removed, and the section was incubated in an Osterix rabbit polyclonal IgG primary antibody (Santa Cruz Biotechnologies A-13, sc-22536) at a 1:300 dilution or a was a rabbit polyclonal to Osteocalcin (ab93876) at a 1:100 dilution in 1% BSA and 1% serum in PBS overnight at 4°C. Tissue sections were then washed three times at 10 minutes per wash in PBS. Tissue sections were then suspended in a solution containing the secondary antibody, donkey anti-rabbit Alexa Fluor 488 (Invitrogen) or goat anti-rabbit Cy5 (Abcam) at a 1:100 dilution in 1% BSA and 1% donkey serum in PBS. Tissue sections were incubated with the secondary antibody at room temperature in the dark for two hours. Sections were then washed three times for ten minutes each in PBS and mounted on slides in 50% PBS-buffered glycerol for imaging.

Strecker S.E., Unterman S., Charles L.F., Pivovarchick D., Maye P.F., Edelman E.R, & Artzi N. (2019). Osterix-mCherry Expression Allows For Early Bone Detection In A Calvarial Defect Model. Advanced biosystems, 3(12), e1900184.

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