Poly l lysine coated culture vessels

Manufactured by Corning

Sourced in United States

Poly-L-lysine-coated culture vessels are laboratory equipment designed to facilitate cell adhesion and growth in in vitro cell culture applications. The poly-L-lysine coating on the surface of the culture vessels provides a favorable substrate for the attachment and proliferation of various cell types.

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Lab products found in correlation

Alveolar epithelial cell medium, by ScienCell (2 mentions) Hpaepic, by ScienCell (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Normal human lung fibroblasts nhlfs, by Lonza (1 mentions) Mle 12, by American Type Culture Collection (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Hek293a cells, by Thermo Fisher Scientific (1 mentions) Poly l lysine coated culture vessels, by ScienCell (1 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Penicillin streptomycin, by ScienCell (1 mentions) Hbmec, by ScienCell (1 mentions) Endothelial cell medium (ecm), by ScienCell (1 mentions) Astrocyte medium, by ScienCell (1 mentions)

3 protocols using poly l lysine coated culture vessels

Culturing Lung Epithelial and Fibroblast Cells

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Human lung adenocarcinoma cells (A549) and mouse alveolar epithelial cells (MLE12) from American Type Culture Collection (Manassas, VA, USA) were cultured in Dulbecco's Modified Eagle Medium (DMEM) and DMEM/Ham's F-12, respectively, supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin). These cells exhibit the cuboidal cell morphology of AEC II. Human pulmonary alveolar epithelial cells (HPAEpiC) from ScienCell Research Laboratories (Carlsbad, CA, USA) were cultured in alveolar epithelial cell medium (ScienCell Research Laboratories) with poly-L-lysine-coated culture vessels (Corning Incorporated, Tewksbury, MA, USA). Normal human lung fibroblasts (NHLFs) from Lonza (Walkersville, MD, USA) were cultured in DMEM supplemented with 10% FBS and antibiotics.

Kato A., Okura T., Hamada C., Miyoshi S., Katayama H., Higaki J, & Ito R. (2014). Cell Stress Induces Upregulation of Osteopontin via the ERK Pathway in Type II Alveolar Epithelial Cells. PLoS ONE, 9(6), e100106.

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Culturing Alveolar Epithelial and HEK293T Cells

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HPAEpiC from ScienCell Research Laboratories (Carlsbad, CA) were cultured in alveolar epithelial cell medium (ScienCell Research Laboratories) with poly-L-lysine-coated culture vessels (Corning, Inc., Tewksbury, MA). HEK293T was originally obtained from the American Type Culture Collection (ATCC) and maintained in DMEM supplemented with 10% FBS, 10 IU/ml penicillin and 10 mg/ml streptomycin. Cells were grown in a humidified atmosphere at 37°C at gas tensions of 20% O₂/5% CO₂ for normoxic incubation. MiR-21 inhibitor, inhibitor-negative control (NC) and Bcl-2 plasmids were purchased from RiboBio (Guangzhou, PR China), nd transfected into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA).

Ge J., Yao Y., Jia H., Li P, & Sun W. (2020). Inhibition of miR-21 ameliorates LPS-induced acute lung injury through increasing B cell lymphoma-2 expression. Innate Immunity, 26(8), 693-702.

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Culturing Human Brain Cell Lines

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HEK293A cells (ThermoFisher Scientific) were maintained in DMEM (ThermoFisher Scientific), 10% fetal bovine serum (FBS) (ThermoFisher Scientific), 0.1 mM MEM non-essential amino acids (ThermoFisher Scientific) and subcultured every 3–5 days. Cells were used below 30 passages. Primary human brain microvascular endothelial cells (HBMEC) isolated from human brain tissue (ScienCell Research Laboratories, Carlsbad, CA) were cultured in endothelial cell growth medium (ECM, ScienCell) containing 5% FBS, 1% endothelial cell growth supplement and 100 units/ml penicillin/streptomycin in poly-L-lysine coated culture vessels (Corning Life Science, Tewksbury, MA). HBMEC cells were subcultured every 3 to 4 days and used between passages 5 and 10. Primary human cortical astrocytes isolated from fetal cerebral cortex (ScienCell) were cultured in astrocyte medium (ScienCell) containing 2% FBS, 1% astrocyte growth supplement and 100 units/ml penicillin/streptomycin in poly-L-lysine coated culture vessels and subcultured every 3 and 4 days and used between passages 5 and 10. All cells were cultured in a 37°C, 5% CO₂ incubator.

Zhang D., Kinloch A.J., Srinath A., Shenkar R., Girard R., Lightle R., Moore T., Koskimäki J., Mohsin A., Carrión-Penagos J., Romanos S., Shen L., Clark M.R., Shi C, & Awad I.A. (2020). Antibodies in Cerebral Cavernous Malformations React with Cytoskeleton Autoantigens in the Lesional Milieu. Journal of autoimmunity, 113, 102469.

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