Oligonucleotides, including miR-1323 mimics, miR-1323 inhibitor, TP53INP1 siRNA, GAS5 siRNA and NC Oligonucleotides, were obtained from GenePharma (Shanghai, China). TP53INP1 3′-UTR and GAS5 sequences including putative miR-1323 binding sites were fused to a modified pcDNA3.1 vector containing sequences encoding a luciferase reporter gene. Reporter plasmids with mutant sites were constructed using Mutagenesis Kit (Stratagene, La Jolla, CA, USA). Oligonucleotides and plasmids were transfected using Hiperfect transfection reagent (Qiagen, Valencia, CA, USA) and Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA), respectively. Luciferase reporter analysis was conducted using Dual Luciferase Assay System (Promega, Madison, WI, USA) according to the manufacturer’s instructions.
Tp53inp1 sirna
Manufactured by GenePharma
Sourced in China
TP53INP1 siRNA is a small interfering RNA (siRNA) designed to target the TP53INP1 gene. TP53INP1 is a stress-induced gene that plays a role in regulating cellular processes. The TP53INP1 siRNA can be used to study the functional effects of silencing the TP53INP1 gene expression in various experimental models.
Automatically generated - may contain errors
Lab products found in correlation
Dual luciferase assay system, by Promega (1 mentions)
Hiperfect transfection reagent, by Qiagen (1 mentions)
Nc oligonucleotides, by GenePharma (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Gas5 sirna, by GenePharma (1 mentions)
Inhibitor control, by GenePharma (1 mentions)
Mir 106b mimics, by GenePharma (1 mentions)
Mimics control, by GenePharma (1 mentions)
Scrambled control sirna, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
3 protocols using tp53inp1 sirna
1
Investigating miR-1323 Regulatory Interactions
2
Transfection of miRNA and siRNA
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The mimics control, miR-106b mimics, inhibitor control, anti-miR-106b inhibitor, scrambled siRNA control, Tp53inp1 siRNA, and Cdkn1a siRNA were purchased from GenePharma (GenePharma Co., Ltd., Shanghai). Transfection of miRNA mimics/inhibitor or siRNAs was performed using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instruction.
3
TCONS_00026334 and TP53INP1 siRNA Transfection
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Oligonucleotides, including the TCONS_00026334 short interfering RNA (siRNA), miR‐548n mimics, TP53INP1 siRNA, and corresponding negative controls, were all synthesized from the GenePharma (Shanghai, China). The TCONS_00026334 cDNA was cloned into the pcDNA3.1 expression vector. GenePharma constructed the TCONS_00026334 cDNA, TP53INP1 cDNA, and the respective negative controls. 0.5 × 105 cells/ml of SW1116 and HCT116 cells were seeded into six‐well plates and subsequently incubated for 24 h before being transfected with the above‐mentioned oligonucleotides and plasmids with Lipofectamine 2000 (Invitrogen).
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