Rabbit IgG (sc-2027), rabbit anti-human CatSper 1 (H300) (sc-33153), rabbit anti-CatSper 1 (Santa Cruz, sc-33153), rabbit anti-human CCR 6 (H-81) (sc-5623), goat anti-human CCR-6 (N-19) (sc-9695), mouse anti-human CCR6 (R&D MAB195), and rabbit anti-human DEFB1 (FL-68) (sc-20797) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant human active DEFB1 (ab50048) was purchased from Abcam (Cambridge, MA). CCL20 (360-MP-025/CF) was purchased from R & D Systems. Quinn’s human tubal fluid (HTF) (ART-1020) media was purchased from SAGE Media. Mouse monoclonal anti-β-actin (1:5000, A1978), CatSper1 ion channel activator Progesterone (P8783), CatSper1 ion channel inhibitor Mibefradil (M5441) and NNC 55-0396 hydrate (N0287) were purchased from Sigma-Aldrich Co (St Louis, MO). Discontinuous density gradient Percoll (17-0891-01) was purchased from GE Healthcare Life Sciences (Pittsburg, PA).
Nnc 55 0396 hydrate n0287
Manufactured by Merck Group
NNC 55-0396 hydrate (N0287) is a chemical compound used in laboratory research applications. It functions as a selective antagonist for the N-type voltage-gated calcium channel. The product is available in a hydrated form.
Automatically generated - may contain errors
Lab products found in correlation
Progesterone p8783, by Merck Group (1 mentions)
Mibefradil m5441, by Merck Group (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Mibefradil dihydrochloride hydrate, by Merck Group (1 mentions)
Lomustine, by Merck Group (1 mentions)
Wst 1 cell proliferation assay kit, by Cayman Chemical (1 mentions)
2 protocols using nnc 55 0396 hydrate n0287
1
Sperm Function Evaluation Reagents
2
Evaluating Cell Viability and Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Metabolic activity of cells as a marker of cell viability and proliferation was assessed by WST-1 Cell Proliferation Assay Kit (10008883, Cayman Chemical, MICH, USA) according to the manufacture's recommendation. Three MB cell lines, D341, CHLA-01, and CHLA-01R, were used in this assay. Brie y, cells were seeded at a density of 30,000 cells/well in 96 well plates. 24 h later, treatment was added as shown in the results section. Assay reaction was assessed 24 or 72 h after treatment. For that, 10 μL of WST-1 reagent was added to each well for 4 h, absorption was measured using a plate reader (Multiskan Go, Thermo Fisher Scienti c, Scoresby, VIC, Australia). Drugs used, including mibefradil dihydrochloride hydrate (M5441), NNC55-0396 hydrate (N0287), verapamil hydrochloride (V4629), nifedipine (N7634), vincristine (V0400000), and lomustine (L5918), were purchased from Sigma-Aldrich.
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