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Gata4 luc

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The GATA4-luc is a reporter gene construct that contains the firefly luciferase gene under the control of the GATA4 promoter. It is used to study the transcriptional activity of the GATA4 gene in cellular systems.

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Lab products found in correlation

Nfat luc, by Promega (2 mentions) Creb1 luc, by Promega (2 mentions) Tk luc, by Promega (2 mentions) Mef2 luc, by Addgene (2 mentions) Veritas 96 well microplate luminometer, by Promega (2 mentions) Xfect reagent, by Takara Bio (2 mentions) Dual luciferase assay kit, by Promega (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions)

2 protocols using gata4 luc

1

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
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2

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
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