Serbp1

For Western blot analysis, HepG2 cells were lysed in the RIPA buffer supplemented with protease inhibitors (Thermo Scientific). Lysates were separated by SDS-PAGE and immunoblotted with antibodies as indicated. Western blot analysis was performed with the following antibodies: SERBP1 (Abcam, ab28481), Fatty acid synthase (Cell Signaling, 3180S), β-actin (Santa Cruz Biotechnology, sc-47778).

The Western blot assay was conducted as previously described.⁹, ¹⁰ Briefly, total protein of mouse kidney tissue and NRK‐52E cells were extracted with Pierce IP Lysis Buffer (Thermo Fisher Scientific) and were quantified with Pierce BCA Protein Assay Kit (Thermo Scientific). A protein of equal amount (60 μg) was separated by 10% SDS‐PAGE and transferred to PVDF membranes. The membranes were blocked with 5% skim milk and then incubated with primary antibodies against SERBP1 (1:2000, Abcam), TGF‐β1 (1:500, Cell Signaling), Fibronectin and Collagen I (1:200, Abcam). The membranes were incubated at 4°C overnight and were then incubated with horseradish peroxidase‐conjugated secondary antibodies at room temperature for 1 hour. The bands were visualized using the Enhanced Chemiluminescence Kit (GE Healthcare) using the GAPDH antibody as an internal control.