Sars cov 2 pseudovirus cov ps02

SARS-CoV-2 Pseudovirus (COV-PS02), expressing S-glycoprotein on the surface of the Lentivirus, and HEK293/ACE2 cells, genetically engineered to overexpress angiotensin-converting enzyme 2, were purchased from Creative Diagnostics (Shirley, NY, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and geneticin (G-418 sulfate) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The Cell Titer-Glo Luminescent cell viability assay kit was purchased from Promega (Madison, WI, USA). HEK293/ACE2 was maintained at 37 °C, 5% CO₂ in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum and 0.5 mg/mL of G418. The cells were sub-cultured within 48 h intervals.

SARS-CoV-2 Pseudovirus (COV-PS02) expressing S-glycoprotein on the surface of the Lentivirus and HEK293/ACE2 cell genetically engineered to overexpress angiotensin-converting enzyme 2 were purchased from Creative Diagnostics (Shirley, NY, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and geneticin (G-418 sulfate) were purchased from Thermo Fisher Scientific (Waltham, MA, USA), and the Cell Titer-Glo Luminescent cell viability assay kit was purchased from Promega (Madison, WI, USA). HEK293/ACE2 was maintained at 37 °C, 5% CO₂ in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum and 0.5 mg mL⁻¹ of G418. The cells were sub-cultured within 48 h intervals.