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5 protocols using alanine aminotransferase

1

Liver Enzyme Activity Assays

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Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) assay kits were supplied by Randox Laboratory (Crumlin, UK). All other reagents were of analytical grade and were prepared in distilled water unless otherwise stated.
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2

Biochemical Assays for Liver Function

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Chemicals and reagents such as AlCl3 and thiobarbituric acid (TBA) were procured from Sigma-Aldrich, Inc., (Saint Louis, MO, USA) while vanillin and caffeine were sourced from British Drug Houses (BDH) Chemical Ltd., Poole, England. Other biochemical assay kits such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), total proteins (TPs), urea, and creatinine assay kits were procured from Randox Laboratory Ltd., Crumlin (Antrim, Northern Ireland, UK).
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3

Comprehensive Biochemical Profiling Protocol

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Total cholesterol, alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin and direct bilirubin, total protein, albumin, urea, creatinine, calcium, sodium, chloride, potassium, and bicarbonate test kits were purchased from Randox Laboratory (United Kingdom).
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4

Sodium arsenite toxicity assessment

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Sodium arsenite (NaAsO2; BDH chemicals Ltd poole England) dosage (1/10th of the oral LD50) was administered according to [32 ]. Also, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphates (ALP), urea and creatinine kits were procured from Randox Laboratories, Crumlin, UK.
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5

Cell Culture and Biochemical Assays

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RPMI 1640 was purchased from GIBCO, gentamicin sulfate, sorbitol and geimsa stain was purchased from Sigma Aldrich, Germany, while aspartate aminotransferase, albumin, alanineaminotransferase, alkaline phosphate, protein and albumin kit were purchased from Randox, London.
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