Keratinocyte sfm k sfm

The immortalized human prostate epithelial cell line (RWPE-1) was obtained from the American Type Culture Collection. RWPE-1 cells were cultured in Keratinocyte-SFM (K-SFM, Invitrogen Co., Carlsbad, CA, USA) supplemented with 0.05 mg/mL bovine pituitary extract (BPE) and 5 ng/mL epidermal growth factor (EGF) in a humidified atmosphere containing 5% CO₂.

Human keratinocytes (HDK1), a cell line established by Egawa et al. [30 (link)], were used as an epidermal stem/progenitor cell model. HDK1 cells have been reported to be epidermal stem cells with a potential to form three-dimensional (3D) reconstructed epidermis in culture and positive for Integrin beta-1 (ITGB1) and Integrin alpha-6 (ITGA6) that are both known as epidermal stem cell markers [31 (link),32 (link)]. SF8428 line established by Ban S et al. was used as a dermal stem/progenitor cell model [33 (link),34 ]. SF8428 has been reported to be mesenchymal stem cell-like fibroblasts with multiple differentiation potentials [35 (link)]. We showed that SF8428 expressed mesenchymal stem cell markers, CD105 and CD44 (Fig. S1). HDK1 and SF8428 were cultured in Keratinocyte-SFM (KSFM; Invitrogen, CA, USA) and in DMEM supplemented with 10% fetal bovine serum (FBS; Sigma–Aldrich, MO, USA), respectively.