Hydrogen peroxide h2o2

Manufactured by Sangon

Sourced in China

Hydrogen peroxide (H2O2) is a clear, colorless liquid chemical compound. It has the molecular formula H2O2 and is a powerful oxidizing agent. Hydrogen peroxide is commonly used as a disinfectant, bleaching agent, and in various chemical processes.

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Lab products found in correlation

Nucleoside, by Merck Group (1 mentions) Resveratrol rsv, by Merck Group (1 mentions) Gkt137831 gkt, by Selleck Chemicals (1 mentions) N acetylcysteine nac, by Beyotime (1 mentions) Cell strainer, by BD (1 mentions) Pifithrin α, by Beyotime (1 mentions) N acetylcysteine nac, by Merck Group (1 mentions) Phi29 dna polymerase, by Thermo Fisher Scientific (1 mentions) Hemin, by Sangon (1 mentions) Sulfuric acid h2so4, by Sangon (1 mentions) Quickblock blocking buffer, by Beyotime (1 mentions) Rapamycin rap, by Selleck Chemicals (1 mentions) Standard msc expansion medium, by Cyagen (1 mentions) Primary antibodies against beclin 1, by Cell Signaling Technology (1 mentions) Cobalt protoporphyrin 9 copp, by Merck Group (1 mentions) Tunel staining, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) β actin, by Proteintech (1 mentions) Ros detection kit, by Beyotime (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Beyotime (1 mentions)

Spelling variants of this product

Hydrogen peroxide (11 citations)

4 protocols using hydrogen peroxide h2o2

Cell Line Characterization and Treatment Protocols

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U2OS, HT1080 and A549 cell lines were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai). The authenticity of cell lines was characterized at the Cell Bank using DNA markers DXS52, Apo-B, MD17S5 and D2S44. All experiments were performed using cells within 10 passages after receipt. U2OS and NHF cells with stable knockdown of p53 or CXCR2 were as previously described^{35 (link)}. Resveratrol (RSV) (Sigma, USA) and GKT137831(GKT) (Selleckchem, USA) were dissolved in dimethyl sulfoxide (DMSO). The final concentration of DMSO in the culture medium was less than 0.05% (v/v). Control cultures received the same amount of DMSO. Nucleosides were purchased from Sigma-Aldrich and dissolved in DMSO to make stock solutions (50 mM). N-acetylcysteine (NAC) was purchased from Beyotime Institute of Biotechnology (China). Hydrogen peroxide (H₂O₂) was from Sangon Biotech (Shanghai, China). Dulbecco’s Minimum Essential Medium (DMEM), fetal bovine serum, were purchased from Gibco (Carlsbad, CA, USA). The cells were maintained in MEM, or DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin in a humidified 5% CO2/95% air atmosphere at 37 °C.

Li B., Hou D., Guo H., Zhou H., Zhang S., Xu X., Liu Q., Zhang X., Zou Y., Gong Y, & Shao C. (2017). Resveratrol sequentially induces replication and oxidative stresses to drive p53-CXCR2 mediated cellular senescence in cancer cells. Scientific Reports, 7, 208.

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Culturing Human and Mouse Cells

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Human osteosarcoma cells U2OS were obtained from the American Type Culture Collection (Manassas, VA). HEK-293 cells were obtained from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing). The immortalized cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 mg/ml penicillin and 100 mg/ml streptomycin. Primary mouse skin (ear) fibroblasts (MSF) were prepared as described [28 (link)]. Briefly, the mouse ears were minced in a well of 12-well plate, treated with collagenase D/dispase at 37°C for 45 min, incubated in 1ml DMEM/10% FBS medium overnight. Cells were obtained by passing the cell suspension through a Cell Strainer (70 mm,Falcon), centrifuged and then incubated in growth medium (DMEM supplemented with 10% FBS, 100 mg/ml penicillin, 100 mg/ml streptomycin and 2.5 mg/ml amphotericin B). All cells were cultured in a humidified atmosphere of 5% CO2 at 37°C. N-acetylcysteine (NAC) was purchased from Sigma Chemical Co (St. Louis, MO). Hydrogen peroxide (H₂O₂) was from Sangon Biotech. Pifithrin-α was purchased from Beyotime.

Xu B., Wang W., Guo H., Sun Z., Wei Z., Zhang X., Liu Z., Tischfield J.A., Gong Y, & Shao C. (2014). Oxidative stress preferentially induces a subtype of micronuclei and mediates the genomic instability caused by p53 dysfunction. Mutation research, 770, 1-8.

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Sensitive Electrochemical Biosensor Assay

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All essential
DNA sequences were synthesized and purified by Shanghai Sangon Biological
Engineering Technology & Services Co., Ltd. (Shanghai, China).
The details of the nucleotide sequences are given in Table S1. 3,3′,5,5′-Tetramethylbenzidine (TMB)
was purchased from Takara Biotechnology (Dalian, China) Co., Ltd.
Hydrogen peroxide (H₂O₂), sulfuric acid (H₂SO₄), and hemin were obtained from Sangon Biotechnology
Co., Ltd. (Shanghai, China). The enzymes, including exonuclease-III
(Exo-III), exonuclease-I (Exo-I), T4 DNA ligase, phi29 DNA polymerase,
and nicking endonuclease (Nb.BbvCI), and the F96 Maxisorpnunc-immuno
plate (96-well plate) were purchased from Thermo Fisher Scientific
Inc. (Shanghai, China). Dimethyl sulfoxide (DMSO) was used to create
a 100 mM hemin stock solution before being further diluted in water.

Shao M., Wang S., Wang B., Guo L., Cui Y, & Wei Y. (2024). Sensitive, Low-Background-Signal miRNA Analysis via the Self-Priming-Initiated Color Reaction Loaded on a Rolling Circle Amplification Product. ACS Omega, 9(5), 5371-5377.

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Hydrogen Peroxide Modulation of Mesenchymal Stem Cells

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Hydrogen peroxide (H₂O₂) was purchased from Sangon Biotech (Shanghai, China). Cobalt protoporphyrin IX (CoPP) was purchased from Sigma (USA). Rapamycin (RAP) was purchased from Selleck (USA). The standard MSC expansion medium was purchased from Cyagen Biosciences Inc. (Guangzhou, China). Cell counting kit-8 (CCK-8) was acquired from Dojindo (Japan). ROS detection kit, TUNEL staining, Annexin V-FITC/PI Apoptosis Detection Kit, and Quick Block™ Blocking Buffer were purchased from Beyotime Institute of Biotechnology (Beyotime, China). The rat heme oxygenase-1- (HO-1-) siRNAs were designed and manufactured by Ribobio Co., Ltd. (Guangzhou, China). The mRFP-GFP-LC3 adenovirus was purchased from HanBio Technology Co., Ltd. (Shanghai, China). Primary antibodies against HO-1, β-actin, and the secondary antibodies were purchased from Proteintech (Wuhan, China). Primary antibodies against LC3-I/II were purchased from Abcam (UK). Primary antibodies against Beclin-1 were purchased from Cell Signaling Technology (USA).

Chen S., Liu S., Zhao L., Lin H., Ma K, & Shao Z. (2020). Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity, 2020, 9349762.

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