Quemesa charge coupled device camera

Manufactured by Olympus

Sourced in Germany

The Quemesa charge-coupled device (CCD) camera is a high-performance imaging device designed for various laboratory applications. It features a CCD sensor that converts light into electrical signals, enabling the capture of detailed and accurate images. The core function of the Quemesa CCD camera is to provide reliable and precise image acquisition for scientific and research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai g2 spirit, by Thermo Fisher Scientific (1 mentions) M7027, by Merck Group (1 mentions) Tecnai spirit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Quemesa camera Quemesa

3 protocols using quemesa charge coupled device camera

NanoSight NTA and TEM Analysis of Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols

NTA was performed with NanoSight LM10‐HS equipped with an automated syringe pump, a temperature sensor and 488 nm laser (NanoSight Ltd.), and software 3.0. Samples were diluted in commercial PBS to a particle concentration within the linear range of the instrument: 2e⁺⁸ – 1 e⁺⁹ particles/mL and a final volume of 1 mL which was taken up with a 1 mL syringe and introduced in the chamber. For every sample, the pump flow was set at 20 and 3 videos of 30 s were recorded with screen gain set at 1.0 and camera level at 13. For processing the videos, the screen gain was set at 1.0 and the detection threshold at 3.0.
Transmission electron microscopy: Samples were deposited on a formvar coated grid, stabilised with evaporated carbon film and glow discharged before sample application. Sample staining with neutral uranyl acetate (2% in aqua distilled (AD)) was done after which grids were coated with 2% methyl cellulose/uranyl acetate (0.4%) solution. These grids were analysed using a Tecnai G2 Spirit transmission electron microscope (FEI) operated at 100 kV and images were acquired with a Quemesa charge‐coupled device camera (Olympus Soft Imaging Solutions GMBH).

Roux Q., Boiy R., De Vuyst F., Tkach M., Pinheiro C., de Geyter S., Miinalainen I., Théry C., De Wever O, & Hendrix A. (2023). Depletion of soluble cytokines unlocks the immunomodulatory bioactivity of extracellular vesicles. Journal of Extracellular Vesicles, 12(8), 12339.

+ Open protocol

+ Expand

Transmission Electron Microscopy for EV Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

EV preparations obtained from blood plasma and urine were qualitatively analyzed with transmission electron microscopy (TEM) (Fig. 1E). Samples were deposited on a formvar coated grids stabilized with evaporated carbon film and glow discharged before sample application (AGS162-3 H, Agar Scientific). Neutral uranyl acetate (2% in AD) (21447-25, Polysciences) was used for staining after which grids were coated with 2% methyl cellulose (M7027, Sigma-Aldrich) / uranyl acetate (0,4%) solution. These grids were examined using a Tecnai G2 Spirit transmission electron microscope (Thermo Fisher Scientific FEI) operated at 100 kV and images were captured with a Quemesa charge-coupled device camera (Olympus Soft Imaging Solutions).
Information on density measurement, interface mixing, and characterization methods (Fig. 1E) of rEV (fNTA and anti-p24 ELISA) and EV (mass-spectrometry based proteomics and TEM) is provided in the Additional file 1.

Van Dorpe S., Lippens L., Boiy R., Pinheiro C., Vergauwen G., Rappu P., Miinalainen I., Tummers P., Denys H., De Wever O, & Hendrix A. (2023). Integrating automated liquid handling in the separation workflow of extracellular vesicles enhances specificity and reproducibility. Journal of Nanobiotechnology, 21, 157.

+ Open protocol

+ Expand

Immunogold Labeling of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated EV were deposited on glow-discharged formvar carbon-coated grids and stained with neutral uranylacetate and embedded in methylcellulose/uranyl acetate. For immune-electron microscopy, the grids containing the vesicles were incubated with 1% BSA in PBS blocking solution. Antibodies and gold conjugates were diluted in 1% BSA in PBS. The grids were exposed to the primary anti-CD63 antibody for 20 min, followed by secondary antibody rabbit anti-mouse IgG (Zymed, San Francisco, CA, USA) for 20 min and protein A-gold complex for 20 min. The grids were examined in a Tecnai Spirit transmission electron microscope (FEI, Eindhoven, The Netherlands). Images were captured by Quemesa charge-coupled device camera (Olympus Soft Imaging Solutions, Munster, Germany).

Geeurickx E., Tulkens J., Dhondt B., Van Deun J., Lippens L., Vergauwen G., Heyrman E., De Sutter D., Gevaert K., Impens F., Miinalainen I., Van Bockstal P.J., De Beer T., Wauben M.H., Nolte-‘t-Hoen E.N., Bloch K., Swinnen J.V., van der Pol E., Nieuwland R., Braems G., Callewaert N., Mestdagh P., Vandesompele J., Denys H., Eyckerman S., De Wever O, & Hendrix A. (2019). The generation and use of recombinant extracellular vesicles as biological reference material. Nature Communications, 10, 3288.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!