Anti mouse and anti rabbit immunoglobulin g conjugated horseradish peroxidase hrp

Cell culture reagents including M-199 medium, L-glutamine, penicillin, streptomycin, and fetal bovine serum were obtained from Gibco BRL (Grand Island, NY, USA). Anti-mouse and anti-rabbit immunoglobulin G-conjugated horseradish peroxidase (HRP) was purchased from Amersham Biosciences (Sunnyvale, CA, USA) and/or Jackson-ImmunoResearch (West Grove, PA, USA). Anti-eNOS, anti-p-NF-κB, anti-cleaved PARP, anti-VCAM-1, anti-Bax, and Bcl2 were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-p42/p44 ERK (Thr202/Tyr204) was from Cell Signaling (Beverly, MA, USA). The Hybond-P polyvinylidene difluoride (PVDF) membrane, enhanced chemiluminescence (ECL) Western blotting detection reagent, and analysis system were obtained from Amersham (Buckinghamshire, UK). All other chemicals used in this study were of reagent grade.

Hinikitiol with more than 90% purity was purchased from Sigma (St. Louis, MO, USA). Sodium dodecylsulfate (SDS), phenylmethylsulfonyl fluoride (PMSF), leupeptin, aprotinin, sodium fluoride, sodium orthovanadate, sodium pyrophosphate, diethyl pyrocarbonate (DEPC), bovine serum albumin (BSA), potassium ferricyanide, ferric chloride, nitroblue tetrazolium (NBT), and riboflavin were all purchased from Sigma-Aldrich (St. Louis, MO, USA). An antibody against MMP-9 was purchased from LabVision/NeoMarkers (Fremont, CA, USA). Antibodies against MMP-2 and active caspase-9 and -3 were purchased from BioVision (Mountain View, CA, USA). Anti-mouse and anti-rabbit immunoglobulin G-conjugated horseradish peroxidase (HRP) was purchased from Amersham Biosciences (Sunnyvale, CA, USA) and/or Jackson-Immuno Research (West Grove, PA, USA). The Hybond-P polyvinylidene difluoride (PVDF) membrane, and enhanced chemiluminescence (ECL) Western blotting detection reagents and analysis systems were obtained from Amersham (Buckinghamshire, UK).

Brazilin (Figure 1(a)) was purchased from ICN Pharmaceuticals (Irvine, CA, USA). Cell culture reagents including M-199 medium, L-glutamine, penicillin, streptomycin, and fetal bovine serum were obtained from Gibco BRL (Grand Island, NY, USA). Antimouse and antirabbit immunoglobulin G-conjugated horseradish peroxidase (HRP) were purchased from Amersham Biosciences (Sunnyvale, CA, USA) and/or Jackson-Immuno Research (West Grove, PA, USA). A rabbit polyclonal antibody specific for NF-kB was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antiphospho-p42/p44 ERK (Thr202/Tyr204) was from Cell Signaling (Beverly, MA, USA). The hybond-P polyvinylidene difluoride (PVDF) membrane and enhanced chemiluminescence (ECL) western blotting detection reagent and analysis system were obtained from Amersham (Buckinghamshire, UK). All other chemicals used in this study were of reagent grade.