Glutathione gsh assay kit

Manufactured by Cayman Chemical

Sourced in United States

The Glutathione (GSH) assay kit is a laboratory tool used to quantify the levels of glutathione, a crucial antioxidant, in biological samples. The kit employs a colorimetric or fluorometric detection method to measure the amount of GSH present in the sample.

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4 protocols using glutathione gsh assay kit

Luteolin Antioxidant and Anti-inflammatory Effects

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Luteolin (>99% pure) was purchased from Sigma (St. Louis, MO, USA), dissolved in DMSO, aliquoted, and stored at −20°C. Potassium dichromate (K₂Cr₂O₇) was obtained from Sigma-Aldrich (St. Louis, MO). Dichlorodihydrofluoresceine acetate (DCFDA) and dihydroethidium (DHE) were obtained from Molecular Probes (Eugene, OR). Lipofectamine 2000 was purchased from Invitrogen Corporation (Carlsbad, CA). Antibodies specific for p-P38, P38, p-AKT, AKT, p-FAK, FAK, p-STAT3, STAT3, Bcl-2, Bcl-xL, iNOS were obtained from cell signaling Technology (Beverly, MA). The primary antibodies specific for p-ERK, ERK, p-JNK, JNK, NF-κB/p65, IKKα, IκBα, COX-2, TNF-α, MMP-9, VEGF, HIF-1-α, β-actin and the secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Glutathione (GSH) assay kit was obtained from Cayman Chemical (Ann Arbor, MI). Assay kit for thiobarbituric acid reactive substances (TBARS) was purchased from BioAssay Systems (Hayward, CA, USA). Luciferase assay kit was obtained from Promega (Madison, WI).

Pratheeshkumar P., Son Y.O., Divya S.P., Roy R.V., Hitron J.A., Wang L., Kim D., Dai J., Asha P., Zhang Z., Wang Y, & Shi X. (2014). Luteolin inhibits Cr(VI)-induced malignant cell transformation of human lung epithelial cells by targeting ROS mediated multiple cell signaling pathways. Toxicology and applied pharmacology, 281(2), 230-241.

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Oxidative stress assessment in La2O3 NPs

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The cell lysate was prepared after exposure to La₂O₃ NPs. The lipid peroxide (LPO) and glutathione (GSH) level were measured as per instructions of lipid hydroperoxide assay kit (item no. 705002) and glutathione (GSH) assay kit (Item no. 703002), respectively, Cayman Chemical, USA.

Alsubaie S.M., Ali D., Almutairi B.O., Almeer R, & Alarifi S. (2022). Evaluation of Cyto - and Genotoxic Influence of Lanthanum Dioxide Nanoparticles on Human Liver Cells. Dose-Response, 20(3), 15593258221128428.

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Biomarker Quantification in Rat Model

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Bovine serum albumin, streptozocin, triethanolamine, and α-chloralose were purchased from Sigma-Aldrich (St. Louis, MO, USA). AssayMax Rat adiponectin enzyme-linked immunosorbent assay (ELISA) kit was purchased from Assaypro (St. Charles, MO, USA). The creatinine reagent set and urea nitrogen reagent set were purchased from Teco Diagnostics (Anaheim, CA, USA). The rat microalbumin ELISA kit was obtained from Kamiya Biomedical Company (Gateway Drive, Seattle, USA).
Rat C-reactive protein (CRP) ELISA kit, rat IL-6 Legend Max^TM ELISA kit, and rat TNF-α Legend Max^TM ELISA kit were obtained from BioLegend (San Diego, CA, USA). Rat monocyte chemoattractant protein-1 (MCP-1) ELISA kit was obtained from RayBiotech (Norccoss, GA, USA). Rat CRP ELISA kit was obtained from Immunology Consultants Laboratory, Inc. (Portland, OR, USA). OxiSelect^TMIn Vitro ROS/RNA assay kit and OxiSelect^TM advanced glycation end competitive ELISA kit were obtained from Cell Biolabs (San Diego, CA, USA). Superoxide dismutase (SOD) assay kit, glutathione (GSH) assay kit, and GSH peroxidase (GPx) assay kit were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). Bio-Rad Protein Assay Kit was obtained from Bio-Rad (Hercules, CA, USA).

Chan K.C., Kok K.E., Huang K.F., Weng Y.L, & Chung Y.C. (2020). Effects of fermented red bean extract on nephropathy in streptozocin-induced diabetic rats. Food & Nutrition Research, 64, 10.29219/fnr.v64.4272.

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Quercetin Antioxidant and Inflammatory Assays

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Reagents and assay kits. Quercetin (C15H10O7, CAS Number 117-39-5) was purchased from Sigma-Aldrich (St. Louis, MO, USA) and was prepared daily and freshly by dissolving it in a normal saline solution (0.9 % NaCl) to the final concentration of 50mg/ml. Assay kits for determination of malondialdehyde (MDA, Cat No. NWK-MDA01) were purchased from NWLSS (Vancouver, BC, Canada). Glutathione (GSH) assay kit was purchased from Cayman Chemical, Cat. No.703002, MI, USA. ELISA kits for determination the levels of IL-6 (Cat No. ELR-IL6-001) was purchased from RayBio, GA, USA. ELISA kits for determination of TNF-a (Cat No. ab46070) was purchased from Abcam, Cambridge, UK. Urea and creatinine were determined by colorimetric assay using assay kits (ab83362 and ab204537) from Abcam, MA, USA.

Bayoumy N. (2020). Quercetin Protects Against Acetaminophen-Induced Acute Nephrotoxicity Associated with the Inhibition of Biomarkers of Acute Kidney Injury in Rats. International Journal of Morphology, 38(4), 876-881.

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