Plate luminometer

Manufactured by Berthold Technologies

Sourced in Germany

The Plate Luminometer is a laboratory instrument designed to measure light output in multi-well plates. It is capable of detecting and quantifying luminescent signals, which can be used to analyze various biological and chemical processes.

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Lab products found in correlation

Accuri c6, by BD (1 mentions) Coelenterazine, by Carl Roth (1 mentions)

Close spelling variants of this product

Mithras LB940 plate luminometer LB960 Centro XS3 plate luminometer Centro LB 960 plate reader luminometer CentroLIApc LB 692 plate luminometer Multi-sample plate-reading luminometer LB 940 plate luminometer 96-well plate luminometer

3 protocols using plate luminometer

Cytotoxicity Screening for Labyrinthopeptins

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Example 9

A cell-based screening system was used to determine the cytotoxic concentration (CC50) of labyrinthopeptin A1 and A2.

1×10⁴HEp-2 cells, which are stably expressing the reporter gene of a firefly luciferase (FF-luc), were seeded in a 96-well plate in 200 μl appropriate media. After 72 h of incubation at 37° C. in the presence of increasing concentrations of labyrinthopeptin A1 or A2 up to 100 μM, the cells have been lysed in 35 μl lysis buffer and the extinction of the FF-luc (RLU) was measured using a plate luminometer (Berthold). The number of surviving cells is indirectly proportional to residual luciferase expression.

The half maximal cytotoxic concentration (CC50) has been calculated for labyrinthopeptin A1 CC50=79.70 μM. There was no cytotoxic affect for labyrinthopeptin A2 detectable up to a concentration of 100 μM.

US11331371B2. Labyrinthopeptins as anti-viral agents (2022-05-17). Helmholtz-Zentrum Für Infektionsforschung GMBH [DE], Medizinische Hochschule Hannover [DE], Twincore, Zentrum Für Experimentelle und Klinische Infektionsforschung GMBH [DE]. Inventors: Mark Brönstrup [DE], Hans-Peter Prochnow [DE], N.V. Suryanarayana Birudukota [DE], Thomas Schulz [DE], Martin Messerle [DE], Thomas Pietschmann [DE], Sibylle Haid [DE], Sebastian Blockus [DE], Christine Laqmani-Goffinet [DE], Sergej Franz [DE], Dominic Howard Banda [BE].

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CD81 Antibody Blocking Impacts on CHIKV and HCV Infection

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For CD81 antibody blocking experiments, adherent Lunet N#3 hCD81 cells were incubated with 5 μg/mL anti-CD81antibody (clone Js-81, BD) or isotype control for 30 min at 37°C. Afterward, cells were infected with a GFP-encoding CHIKV (ECSA genotype) or HCV-JcR2a in the presence of CD81 antibody or isotype control for 4 h at 37°C. Cells were washed twice with phosphate-buffered saline (PBS), fresh medium was added, and infection was stopped after 24 h for CHIKV and 48 h for HCV. For CHIKV, GFP-positive cells were measured by flow cytometry (Accuri C6, BD) as readout for infection. HCV infectivity was determined by luciferase activity using coelenterazine as the substrate and a plate luminometer (Berthold).

Lasswitz L., Zapatero-Belinchón F.J., Moeller R., Hülskötter K., Laurent T., Carlson L.A., Goffinet C., Simmons G., Baumgärtner W, & Gerold G. (2022). The Tetraspanin CD81 Is a Host Factor for Chikungunya Virus Replication. mBio, 13(3), e00731-22.

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CD302 Overexpression Luciferase Assay

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We seeded 1 × 10⁴ Huh-7.5 cells overexpressing CD302, its mutants, or an empty vector control per well in 96-well plates. On the following day, cells were infected, and medium was changed 4 h later and incubated for 72 h. To analyze luciferase activity, cells were washed once with PBS and lysed with 35 μL water, and plates were frozen down at −80°C until they were measured with a plate luminometer (Berthold, Bad Wildbad, Germany) and coelenterazine (Carl Roth GmbH & Co. KG; in methanol) diluted 1:1,000 in PBS.

Reinecke B., Frericks N., Lauber C., Dinkelborg K., Matthaei A., Vondran F.W., Behrendt P., Haid S., Brown R.J, & Pietschmann T. (2022). The Human Liver-Expressed Lectin CD302 Restricts Hepatitis C Virus Infection. Journal of Virology, 96(7), e01995-21.

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