All the chemicals and solvents were of analytical grade and purchased from commercial suppliers. They were used as received without further purification unless otherwise stated. As for the selectivity study, KCl, CaCl2, FeCl3, CuSO4, MgSO4, NaCl, Na2SO4, NaNO2, Na2CO3, NH4Cl, ammonia, hydroxylamine, diethylamine, ethylenediamine, aniline, thiourea, l -Ile, l -Phe, l -Pro, l -His, l -Arg, l -Lys, isoniazid, acethydrazide were used to prepare the stock solutions. Ultrapure water was purified from Millipore water purification system and used throughout all the experiments. Silica gel P60 (Qingdao, mesh number 200–300) was used for column chromatography. 1H NMR and 13C NMR spectra were recorded on a Bruker 400 M instrument and chemical shifts were given in ppm. Mass spectra (ESI) were measured on a Finnigan LCQ DECA spectrometer. UV-vis and fluorescence spectra were recorded on a SHIMADZU UV-2450 spectrophotometer and a VARIAN Cary Eclipse FL1003 M013 spectrometer respectively. The pH value was measured using a pHS-25 digital pH meter. H1975 cells were obtained from American Type Culture Collection (ATCC) and their fluorescence images were captured with a ZEISS LSM 880 Laser scanning confocal microscope.
Eclipse fl1003 m013 spectrometer
Manufactured by Agilent Technologies
The Eclipse FL1003 M013 is a spectrometer designed for laboratory applications. It is capable of measuring the absorption, emission, and fluorescence properties of various samples. The spectrometer features an optical design that allows for accurate and reliable measurements across a wide range of wavelengths.
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2 protocols using eclipse fl1003 m013 spectrometer
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Selectivity Study of Chemical Reagents
2
Fluorescent Probe Synthesis and Cellular Imaging
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All of the chemical reagents and solvents were obtained commercially (Adamas Reagents or Cologne Reagents) and used as received without further purification unless otherwise stated. Ultrapure water was purified using a Millipore water purification system. Silica gel P60 (Qingdao, mesh number 200–300) was used for column chromatography. 1H NMR and 13C NMR spectra were recorded on a Bruker 400 M instrument and chemical shifts are given in ppm using the peak of residual proton signals of DMSO-d6 or CDCl3-d6 as the internal standard. The mass spectra (ESI) were recorded on a Finnigan LCQDECA spectrometer with ESI mode. UV-Vis and fluorescence spectra were recorded on a SHIMADZU UV-2450 spectrophotometer and a VARIAN Cary Eclipse FL1003 M013 spectrometer, respectively. The fluorescence images of the MDA-MB-231 cells were recorded on an inverted fluorescence microscope (Olympus, Japan). The fluorescence images of the living mice were recorded on a Night OWL LB983 living imager.
All of the animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Sichuan University and approved by the Animal Ethics Committee of Sichuan University.
All of the animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Sichuan University and approved by the Animal Ethics Committee of Sichuan University.
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